Serum Paraoxonase 1 Activity Is Associated with Fatty Acid Composition of High Density Lipoprotein

Abstract

Introduction. Cardioprotective effect of high density lipoprotein (HDL) is, in part, dependent on its related enzyme, paraoxonase 1 (PON1). Fatty acid composition of HDL could affect its size and structure. On the other hand, PON1 activity is directly related to the structure of HDL. This study was designed to investigate the association between serum PON1 activity and fatty acid composition of HDL in healthy men. Methods. One hundred and forty healthy men participated in this research. HDL was separated by sequential ultracentrifugation, and its fatty acid composition was analyzed by gas chromatography. PON1 activity was measured spectrophotometrically using paraxon as substrate. Results. Serum PON1 activity was directly correlated with the amount of stearic acid and dihomo-gamma-linolenic acid (DGLA). PON1/HDL-C was directly correlated with the amount of miristic acid, stearic acid, and DGLA and was inversely correlated with total amount of ω6 fatty acids of HDL. Conclusion. The fatty acid composition of HDL could affect the activity of its associated enzyme, PON1. As dietary fats are the major determinants of serum lipids and lipoprotein composition, consuming some special dietary fatty acids may improve the activity of PON1 and thereby have beneficial effects on health.

Avocado and Cardiovascular Health

“…The cardioprotector effect of HDL-cholesterol is in part due of PON1 activity, which is responsible for the hydrolysis of lipid hydroperoxides (products of the lipid oxidation) [33] [34]. About LDL-cholesterol particles, a randomized controlled trial was conducted among 45 overweight/obese participants submitted to a 2-week run-in diet (Average American Diet, AAD) and later allocated to three distinct diets: 1) LF-low fat diet (24% of daily total calories from fats); 2) MF-moderate fat diet (34% of daily total calories from fats); or 3) AVavocado diet (~140 g of Hass avocado a day 13 g of MUFA).…”

HDL Particle Size and Its Association with Paraoxonase - 1 Activity in CAD Patients

“…According to Boshtam et.al. , serum paraoxonase activity directly correlates with the amount of saturated fatty acids like stearic acid and dihomo-gamma-linolenic acid (DGLA) present in HDL [11].These fatty acids are present in the phospholipids and apolipoproteins found in HDL. A study by Razavi et.al.…”

“…Alteration of the fatty acid content of HDL leads to structural changes in the HDL molecule, including changes in its size and zeta potential. This leads to decreased paraoxonase-1 activity, by altering the phospholipid composition of HDL [11].…”

“…These are saturated fatty acids. Any alteration in the fatty acid composition of HDL leads to a decrease in serum PON-1 activity [11].…”

“…This leads to decreased paraoxonase-1 activity, by altering the phospholipid composition of HDL [11]. Loss of Apo-A1 content of HDL is the mainstay in the development of atherosclerosis with reference to HDL.…”