The relationship between sirtuin 1 (SIRT1) expression and tumor size, Proliferating Cell Nuclear Antigen (PCNA) expression and histological grading in rat breast carcinoma induced by dimethylbenz( α )anthracene (DMBA)

Padauleng, Novrita; Purnomosari, Dewajani; Herwiyanti, Sri; Harjadi,; Irianiwati, .; Widyarini, Sitarina

doi:10.19106/jmedscie004504201301

Cited by 1 publication

(3 citation statements)

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“…17 This is consistent with tumorigenesis process, that proved with nodules formation in DMBAinduced Sprague Dawley. 12,18 Similar results were obtained by Padauleng et al, 18 which discovered that SIRT1 expression on DMBAinduced rat was higher than the un-induced ones. Furthermore, Padauleng et al, 18 found that SIRT1 expression was observed in poor histological grade carcinoma SD rats and there was a positive correlation between size of the nodules and PCNA proliferation marker with SIRT1 expression.…”

Section: Discussionsupporting

confidence: 59%

“…In addition, DMBA-induced rats had positive breast carcinoma, characterized by the formation of nodules. 12,18 It proves that NF-κB expression is high in SD rats with breast tumors. These results are consistent with study conducted by Rahman et al, 23 who reported that presentation of NF-κB expression in DMBA-induced rats is higher than control group rat (corn oil-only).…”

Section: Discussionmentioning

confidence: 93%

“…This phenomenon proves the connection between SIRT1 with the development of breast cancer. 18 SIRT1 mechanism in breast cancer development associated to the ability to deacetylate some molecules including tumor suppressor gene p53, 5 p73, 6 and HIC1. 19 In addition to the capability in deacetylating tumor suppressor molecules, SIRT1 also able to regulate aromatase cytochrome p450 enzyme, including CYP19A1 group.…”

Section: Discussionmentioning

confidence: 99%

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Effect of 1.2-epoxy-3[3-3[3.4-dimetoxyiphenil]-4h-1-benzopiran-4-on] Propane (epi) on Sirtuin-1 and Nuclear Factor-kB Expression of DMBA induced Mammary Tumors in sprague dawley rats

2016

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The main factors contribute to breast cancer development is the combination of exogenous and endogenous factors. Endogenous factors include both SIRT1 and NF-kB. Exogenous factor used in this research is 7.12 dimethylbenz(a)anthracene (DMBA). 1,2-epoxy-3[3-3[3,4-dimetoxy-phenil]-4h-1-benzophiran-4-on] propane (EPI) is a derivative of isoflavone generate from clove leaf oil. To examine the effect of EPI on SIRT1 and NF-kB expression in DMBA-induced Sprague Dawley (SD) rats, and the correlation between SIRT1 and NF-kB expressions. Tissue generated form 35 Sprague Dawley female rats aged 2 weeks old were used in this study. Those rats were divided into 7 groups (5 rats/group), namely normal control group; corn oil group; DMBA group; EPI treated groups with 1 mg/kgBW (EPI I), 2 mg/kgBW (EPI II), and 4 mg/kgBW (EPI III), respectively; and doxorubicin group. EPI and doxorubicin were administered from 1 st until 15 th week, while DMBA were administered from 3 rd until 9 th week. The paraffin block was prepared from all breast organ of the rats that terminated at the end of week 15 th. Examination of SIRT1 and NF-kB expression was performed using light microscope at 400x magnifications, after immunohistochemistry (IHC) staining. Expression level of SIRT1 and NF-kB were analyzed using IHC-profiler plug-in in ImageJ software. SIRT1 and NF-kB expression in EPI treated groups were not significantly different with the one in Doxorubicin group, but lower than DMBA group (p=0.000). There was a positive correlation between SIRT1 and NF-kB expression (p=0.001; r=0.773) in EPI-treated group. EPI was able to prevent an increasing of SIRT1 and NF-kB expression in SD model breast cancer that induced with DMBA. There is a positive correlation between SIRT1 and NF-kB expression in EPI-treated SD rats that were induced by DMBA. ABSTRAK Faktor utama penyebab kanker payudara adalah kombinasi faktor endogen dan eksogen. Faktor endogen antara lain adalah ekspresi SIRT1 dan NF-kB dan Faktor eksogen yang digunakan untuk penelitian ini adalah 7.12 dimethylbenz(a)anthracene (DMBA). 1,2-epoxy-3[3-3[3,4-dimetoxy-phenil]-4h-1-benzophiran-4-on] propane (EPI) merupakan turunan isoflavone yang disintesis dari minyak daun cengkeh. Untuk mengkaji pengaruh EPI dengan kelompok Doxorubicin, tetapi lebih rendah daripada kelompok DMBA (p=0.000). Terdapat korelasi positif antara tingkat ekspresi SIRT1 and NF-kB (p=0.001; r=0.773) pada kelompok perlakuan EPI. EPI dapat mencegah peningkatan ekspresi SIRT1 and NF-kB pada model tikus kanker payudara yang diinduksi dengan DMBA. Terdapat korelasi positif antara tingkat ekspresi SIRT1 and NF-kB (p=0.001; r=0.773) pada tikus model yang diberi EPI.

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