The receptor for activated protein kinase C promotes cell growth, invasion and migration in cervical cancer

Liao, Shan; Xiao, Songshu; Chen, Hongxiang; Zhang, Manying; Chen, Zhifang; Long, Yuehua; He, Junyu; Ge, Yanshan; Yi, Wei; Wu, Minghua; Li, Guiyuan; Zhou, Yanhong

doi:10.3892/ijo.2017.4137

Cited by 20 publications

(21 citation statements)

References 53 publications

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“…31,32 For the CCK8 assay, 1 × 10 4 cells were grown in 96-well plates in which CCK-8 reagent was added according to the standard protocol (Beyotime, Beijing, China). 31,32 For the CCK8 assay, 1 × 10 4 cells were grown in 96-well plates in which CCK-8 reagent was added according to the standard protocol (Beyotime, Beijing, China).…”

Section: Cell Proliferation Migration and Invasion Assaysmentioning

confidence: 99%

Long noncoding RNA ZFAS1 promotes tumorigenesis and metastasis in nasopharyngeal carcinoma by sponging miR‐892b to up‐regulate LPAR1 expression

Peng

Liu

Zheng

et al. 2019

J Cellular Molecular Medi

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Objective: In this study, we explored the NPC-specific expression of ZFAS1 and the mechanism of ZFAS1-mediated growth, aggressiveness and tumorigenesis in NPC. Methods:The expression profile of lncRNAs was detected in NPC tissues and matching para-carcinoma tissues using microarray analysis. LncRNA-miRNA and miRNA-mRNA interaction networks were constructed using the miRcode v11 and TargetScanHuman v7.2 web server and then validated using dual-luciferase assay.Western blot and RT-qPCR were performed to detect protein and RNA expression.The effects of ZFAS1, miR-892b and LPAR1 dysregulation on the proliferative, migratory and invasive abilities of NPC cells were observed using colony formation, cell counting kit-8 (CCK-8) and transwell assays in vitro. In vivo, a xenograft nude mouse model was established to detect the impact of ZFAS1 dysregulation on the tumorigenicity of NPC cells. Results:The expression of multiple lncRNAs, of which ZFAS1 was up-regulated, was dysregulated in NPC tissues. ZFAS1 directly targeted miR-892b, and miR-892b negatively regulated the expression of downstream LPAR1. The proliferation, migration and invasion of NPC cells could be largely enhanced by the downregulation of miR-892b as well as the up-regulation of ZFAS1 and LPAR1, while the overexpression of miR-892b and the downregulation of ZFAS1 and LPAR1 decreased these abilities.In nude mice, the growth of tumour xenografts formed by HONE1 cells was significantly suppressed when ZFAS1 was silenced. Conclusion:The study demonstrated that lncRNA ZFAS1 may act as a promoter of tumorigenesis and metastasis in nasopharyngeal carcinoma, by up-regulating the expression of LPAR1 in a miR-892b-dependent manner. K E Y W O R D Slong noncoding RNAs, LPAR1, miR-892b, nasopharyngeal carcinoma, ZFAS1

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Section: Cell Proliferation Migration and Invasion Assaysmentioning

confidence: 99%

Long noncoding RNA ZFAS1 promotes tumorigenesis and metastasis in nasopharyngeal carcinoma by sponging miR‐892b to up‐regulate LPAR1 expression

Peng

Liu

Zheng

et al. 2019

J Cellular Molecular Medi

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“… 10 , 11 Worldwide, a large number of new cervical cancer cases are diagnosed every year, and more than half of patients diagnosed died from the cancer. 3 Tumor progression involves tumor cell proliferation and metastasis and novel targeted therapeutic strategies are urgently required for patients with cervical cancer.…”

Section: Discussionmentioning

confidence: 99%

“…Cervical cancer is the second most common malignancy in women with an estimated 530,000 new cases and 275,000 deaths annually worldwide, 1 , 2 and the majority of female cancer deaths occur in developing countries. 3 Various treatments for cervical cancer are available, according to tumor grade and stage, including surgery and radiation therapy. However, treatment for advanced stage cervical cancer remains a public health concern.…”

Section: Introductionmentioning

confidence: 99%

<em>SMAGP</em> a novel biomarker of cervical cancer development and progression

Jia

Liu

et al. 2018

OTT

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IntroductionCervical cancer, one of the most common malignant gynecological tumors, is a significant burden on the health of females worldwide. The purpose of this study was to investigate genes associated with lymph node metastasis in cervical cancer.MethodsWe report on the lymph node metastasis-associated gene, small cell adhesion glycoprotein (SMAGP), as a key regulator of cervical cancer development and progression. SMAGP expression levels were investigated in 70 cervical squamous cell carcinoma samples and 10 normal cervical squamous epithelium samples.ResultsImmunohistochemistry analysis revealed that SMAGP protein levels were significantly elevated in cervical cancer tissue compared with normal cervical squamous epithelium. Silencing of SMAGP induced cell cycle arrest, inhibited the cell proliferation and colony formation ability of cervical cancer cells in vitro and suppressed their tumorigenic potential in nude mice. In addition, SMAGP knockdown reduced expression of epithelial mesenchymal transition-related proteins, including vimentin, β-cadherin, MMP2, and Twist.ConclusionTogether, our findings demonstrate that SMAGP plays a critical role in cell proliferation and tumorigenesis and could be a new therapeutic target in cervical cancer.

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“…Importantly, a favorable prognosis and increased long-term survival time are correlated with early CC diagnosis and appropriate therapy [15]. Several studies have demonstrated that abnormal molecular biology can predict the prognosis of tumor patients [16][17]. However, in these studies, no single bio-maker that was used to detect CC was both highly sensitive and specific.…”

Section: Discussionmentioning

confidence: 99%

“…Higher GNB2L1 expression was associated with an advanced FIGO stage, lymph node metastasis, and the degree of cell differentiation. GNB2L1 is reported to be upregulated in multiple cancers including oral squamous carcinoma [19], hepatocellular carcinoma [20], and pancreatic ductal adenocarcinoma [11]. A previous study by Liao and colleagues indicated that overexpression of GNB2L1 promotes CC cell proliferation and invasion by affecting the p53 signaling pathway [21].…”

Section: Discussionmentioning

confidence: 99%

Overexpression of GNB2L1 is associated with poor prognosis in cervical cancer patients

Qi¹,

Xu²,

Tuerxun³

et al. 2019

Preprint

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Background: Among women, cervical cancer (CC) is the fourth most prevalent cancer worldwide. Multiple bio-makers have been identified for tumor diagnosis and prognosis. However, no single bio-maker for CC detection exists that is both highly sensitive and specific. In this report, we explored the role of guanine nucleotide-binding protein subunit beta-2-like 1 (GNB2L1) in CC progression in Uyghur women. Methods: In the present study, 132 patients with cervical squamous cell carcinoma (CSCC), 56 cases with cervical intraepithelial neoplasia (CIN) were enrolled. GNB2L1 mRNA and protein expression levels were analyzed in CSCC, CIN, and normal cervical (NC) tissues and in CC cell lines using qRT-PCR, western blot, and immunohistochemistry. Stable GNB2L1-knockdown CC cells (shGNB2L1) were also created and lipid accumulation was measured with Oil Red O staining. Additionally, changes in GNB2L1 gene copy number were investigated with qRT-PCR and sequencing. A gene regulatory network was constructed using the STRING database. Finally, patient survival was assessed with Kaplan‑Meier and log-rank tests. Results: Our results indicated that GNB2L1 mRNA and protein are overexpressed in CSCC tumor tissues when compared to CIN and NC tissues. GNB2L1 expression was associated with the degree of cell differentiation (P = 0.001), lymph node metastasis (P = 0.049), and the clinical stage (P = 0.017). Oil Red O staining showed that lipid levels were lower in shGNB2L1 cells, and that GNB2L1 DNA copy number in CC tissues (29,7200 ± 9,815.061) was significantly higher when compared to NC tissues (19,5100 ± 5,676.392 [T=-2.770, P = 0.009]). Furthermore, patients with CSCC and high GNB2L1 expression demonstrated shorter overall survival times when compared to patients with low GNB2L1 expression (P = 0.002). Multivariate analysis indicated that GNB2L1 overexpression is an independent prognostic factor in patients with CSCC. Conclusions: Overexpression of GNB2L1 may contribute to tumor progression; therefore, it is a potential prognostic biomarker for CSCC patients.

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The receptor for activated protein kinase C promotes cell growth, invasion and migration in cervical cancer

Cited by 20 publications

References 53 publications

Long noncoding RNA ZFAS1 promotes tumorigenesis and metastasis in nasopharyngeal carcinoma by sponging miR‐892b to up‐regulate LPAR1 expression

Long noncoding RNA ZFAS1 promotes tumorigenesis and metastasis in nasopharyngeal carcinoma by sponging miR‐892b to up‐regulate LPAR1 expression

<em>SMAGP</em> a novel biomarker of cervical cancer development and progression

Overexpression of GNB2L1 is associated with poor prognosis in cervical cancer patients

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