Long noncoding RNA ZFAS1 promotes tumorigenesis and metastasis in nasopharyngeal carcinoma by sponging miR‐892b to up‐regulate LPAR1 expression

Abstract

Objective: In this study, we explored the NPC-specific expression of ZFAS1 and the mechanism of ZFAS1-mediated growth, aggressiveness and tumorigenesis in NPC. Methods:The expression profile of lncRNAs was detected in NPC tissues and matching para-carcinoma tissues using microarray analysis. LncRNA-miRNA and miRNA-mRNA interaction networks were constructed using the miRcode v11 and TargetScanHuman v7.2 web server and then validated using dual-luciferase assay.Western blot and RT-qPCR were performed to detect protein and RNA expression.The effects of ZFAS1, miR-892b and LPAR1 dysregulation on the proliferative, migratory and invasive abilities of NPC cells were observed using colony formation, cell counting kit-8 (CCK-8) and transwell assays in vitro. In vivo, a xenograft nude mouse model was established to detect the impact of ZFAS1 dysregulation on the tumorigenicity of NPC cells. Results:The expression of multiple lncRNAs, of which ZFAS1 was up-regulated, was dysregulated in NPC tissues. ZFAS1 directly targeted miR-892b, and miR-892b negatively regulated the expression of downstream LPAR1. The proliferation, migration and invasion of NPC cells could be largely enhanced by the downregulation of miR-892b as well as the up-regulation of ZFAS1 and LPAR1, while the overexpression of miR-892b and the downregulation of ZFAS1 and LPAR1 decreased these abilities.In nude mice, the growth of tumour xenografts formed by HONE1 cells was significantly suppressed when ZFAS1 was silenced. Conclusion:The study demonstrated that lncRNA ZFAS1 may act as a promoter of tumorigenesis and metastasis in nasopharyngeal carcinoma, by up-regulating the expression of LPAR1 in a miR-892b-dependent manner. K E Y W O R D Slong noncoding RNAs, LPAR1, miR-892b, nasopharyngeal carcinoma, ZFAS1

Circ_0001175 Promotes Hepatocellular Carcinoma Cell Proliferation and Metastasis by Regulating miR-130a-5p

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“…The potential miRNAs which may have binding sites with LINC00958 were predicted using the bioinformatics tool StarBase version 3.0 ( ) ( 19 ). Wild-type and mutant reporter plasmids of LINC00958 (WT-LINC00958 and MUT-LINC00958), which contained miR-3619-5p mimic or miR-NC binding sites, were synthesized by Shanghai GenePharma Co., Ltd. (cat.…”

LncRNA ZFAS1 contributed to irradiation resistance in nasopharyngeal carcinoma by inhibiting hsa-miR-7-5p/ENO2

“…There were some researches reporting that the expression of ZFAS1 was aberrant in hepatocellular carcinoma, prostate cancer, colorectal cancer and esophageal squamous cell carcinoma [16][17][18][19]. In our previous study, we also reported that lncRNA ZFAS1 participated in the development of NPC as a tumor promoter [20]. However, we hadn't demonstrated whether ZFAS1 played a role in radio resistance in NPC.…”

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