Circ_0001175 Promotes Hepatocellular Carcinoma Cell Proliferation and Metastasis by Regulating miR-130a-5p

Li, Liheng; He, Kui; Chen, Siliang; Wei, Wenjiang; Tian, Zuofu; Tang, Yinghong; Xiao, Chengjiang

doi:10.2147/ott.s262408

Cited by 15 publications

(8 citation statements)

References 40 publications

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“…Previous studies have reported that miR-130a-5p functioned as a tumor suppressor in HCC, 34 and our study consistently confirmed miR-130a-5p inhibitor and mimic promoted and suppressed the proliferation of HCC cells, respectively. More importantly, the promoted proliferation induced by miR-130a-5p inhibitor and inhibited proliferation induced by miR-130a-5p mimic in HCC cells were blocked by CCNT2 knockdown and overexpression, respectively ( Figure 4F and G ).…”

Section: Resultssupporting

confidence: 87%

Nanoparticles Mediated circROBO1 Silencing to Inhibit Hepatocellular Carcinoma Progression by Modulating miR-130a-5p/CCNT2 Axis

Meng¹,

Li²,

Xie³

et al. 2023

IJN

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Background Circular RNAs (circRNAs) are becoming vital biomarkers and therapeutic targets for malignant tumors due to their high stability and specificity in tissues. However, biological functions of circRNAs in hepatocellular carcinoma (HCC) are still not well studied. Methods Gene Expression Omnibus (GEO) database and qRT-PCR were used to evaluate expression of circROBO1 (hsa_circ_0066568) in HCC tissues and cell lines. CCK-8, colony formation, EdU staining, flow cytometry for cell cycle analysis, and xenograft model assays were performed to detect the circROBO1 function in vitro and in vivo. RNA pull-down, RNA immunoprecipitation (RIP), and Luciferase reporter assays were used to investigate the relationship among circROBO1, miR-130a-5p, and CCNT2. More importantly, we developed nanoparticles made from poly lactic-co-glycolic acid (PLGA) and polyethylene glycol (PEG) chains as the delivery system of si-circROBO1 and then applied them to HCC in vitro and in mice. Results circROBO1 was obviously upregulated in HCC tissues and cell lines, and elevated circROBO1 was closely correlated with worse prognosis for HCC patients. Functionally, knocking down circROBO1 significantly suppressed HCC cells growth in vitro and in mice. Mechanistically, circROBO1 acted as a competing endogenous RNA to downregulate miR-130a-5p, leading to CCNT2 expression upregulation. Furthermore, miR-130a-5p mimic or CCNT2 knockdown reversed the role of circROBO1 overexpression on HCC cells, which demonstrated that circROBO1 promoted HCC development via miR-130a-5p/CCNT2 axis. In addition, we developed nanoparticles loaded with si-circROBO1, named as PLGA-PEG (si-circROBO1) NPs, which significantly prevented the proliferation of HCC cells, and did not exhibit apparent toxicity to major organs in vivo. Conclusion Our findings firstly demonstrate that circROBO1 overexpression promotes HCC progression by regulating miR-130a-5p/CCNT2 axis, which may serve as an effective nanotherapeutic target for HCC treatment.

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Section: Resultssupporting

confidence: 87%

Nanoparticles Mediated circROBO1 Silencing to Inhibit Hepatocellular Carcinoma Progression by Modulating miR-130a-5p/CCNT2 Axis

Meng¹,

Li²,

Xie³

et al. 2023

IJN

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show abstract

“…For instance, circ-0001175 reduces miRNA-130a-5p expression to upregulate SNX5 that is of importance for promoting proliferation rate of HCC cells. 33 Down-regulation of RSK2 due to mutations result in MAPK signaling activation, promoting cholesterol metabolism and subsequent increase in proliferation of HCC cells. 34 Therefore, a variety of genetic and epigenetic alterations can mediate the progression of HCC cells.…”

Section: Introductionmentioning

confidence: 99%

Wnt/β-Catenin Signaling as a Driver of Hepatocellular Carcinoma Progression: An Emphasis on Molecular Pathways

Paskeh

Mirzaei

Ashrafizadeh

et al. 2021

JHC

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“…Circ-ZKSCAN1 restrained the progression of HCC through acting as the molecular sponge of miR-873-5p [ 36 ]. Circ_0001175 contributed to HCC development through sponging miR-130a-5p [ 37 ]. Circ_0091579 was identified to be an oncogene in liver cancer by previous studies [ 7 , 8 , 38 ].…”

Section: Discussionmentioning

confidence: 99%

Circ_0091579 exerts an oncogenic role in hepatocellular carcinoma via mediating miR-136-5p/TRIM27

et al. 2022

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Circ_0001175 Promotes Hepatocellular Carcinoma Cell Proliferation and Metastasis by Regulating miR-130a-5p

Cited by 15 publications

References 40 publications

Nanoparticles Mediated circROBO1 Silencing to Inhibit Hepatocellular Carcinoma Progression by Modulating miR-130a-5p/CCNT2 Axis

Nanoparticles Mediated circROBO1 Silencing to Inhibit Hepatocellular Carcinoma Progression by Modulating miR-130a-5p/CCNT2 Axis

Wnt/β-Catenin Signaling as a Driver of Hepatocellular Carcinoma Progression: An Emphasis on Molecular Pathways

Circ_0091579 exerts an oncogenic role in hepatocellular carcinoma via mediating miR-136-5p/TRIM27

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