The Reactions of the Bennett—Goodspeed Group of Antibodies Tested with the AutoAnalyzer

Following an attack of infectious mononucleosis, the red cells of HLA B7 patients may show a greatly increased reactivity with anti-Bg^a antibodies. This occurs from about the 3rd week of the disease, the reactivity then slowly decreasing over a period of months or years. Both specific and nonspecific reactions to other HLA antisera may also occur. Five patients were followed in detail and the reason for the increase in antigen strength was investigated without a conclusive result.

mentioning

confidence: 93%

Increase in Strength of Red Cell Bg^a Antigen Following Infectious Mononucleosis

Morton¹,

Pickels²,

Darley³

1977

“…Red cell Bg3-grouping was carried out on the Auto Analyser as previously re ported [12] HL-A groups were determined by a cytotoxic method. Lymphocyte suspensions were prepared from defibrinated blood according to the method of Rabinowitz [9].…”

Section: Methodsmentioning

confidence: 99%

“…Tests on the HL-A7 serum D66 15903 II obtained from the NIH Washington has [15] shown weaker but similarly reacting cytotoxic and haemagglutinating antibodies. The Bga (Bennett-Goodspeed) baemagglutinins [1,2] were shown by Seaman et al [12] to react with the red cells of 30% of the popula tion when tested with the Auto Analyser, whereas previously only weak inconsistent results had been obtained with less than 5% of the random population when standard manual methods were used. Three antigens were defined corresponding to the Bennett-Goodspeed [1,2], Ho [7] and Ot [4] groups.…”

mentioning

confidence: 99%

The Correlation of the Bga Blood Group with the HL-A7 Leucocyte Group: Demonstration of Antigenic Sites on Red Cells and Leucocytes

Morton¹,

Pickels²,

Sutton³

1969

Antigenic sites of Bg^a specificity have been demonstrated on leucocytes as well as on red cells. The Bg^a group shows almost complete concordance with the histocompatibility group HL-A7. Red cells show much less activity than leucocytes and did not absorb cytotoxic antibodies but leucocytes removed all haemagglutinating activity. RNAP reactions can be demonstrated by both haemagglutination and cytotoxic tests. In some instances multispecific cytotoxic antibodies may show a monospecific haemagglutinating component. Family studies showed that the HL-A groups may influence the Bg^a reaction of the red cells.

“…If some HLA antigens are present in sufficient amounts on the red cells of certain donors, there is a possibility that even pure red cell transfusion might sensitise. Red cell survival studies have been carried out where HLA antibodies, or Bg antibodies, have been involved [3,12,14]. The results have been inconsistent, and a systematic study including strong, weak and negative reactors should be carried out.…”

Section: Introductionmentioning

confidence: 99%

Association between HLA and Red Cell Antigens

Nordhagen¹

1977

Cytotoxic HLA antisera of various specificities were examined on the AutoAnaly- zer against red blood cells (RBC) from a great number of HLA-typed donors. HLA-associated haemagglutinins of different specificities were demonstrated. In cytotoxic anti-HLA-A9 and anti-HLA-A10, as well as in anti-HLA-B12, anti-HLA-Bwl5 and anti-HLA-B17 sera, haemagglutinins of apparently corresponding specificities were found. This indicates that several HLA antigens may be present on RBC, but they are demonstrable only on RBC from a limited number of donors who possess the corresponding antigens on their white cells. Some antigens, like HLA-A28 and HLA-B7, are relatively strong RBC antigens, whereas others, like HLA-B12 or HLA-Bwl5 seem to be much weaker. In cytotoxic anti-HLA-Al and anti-HLA-B13 sera, no corresponding haemagglutinins were demonstrated. However, haemagglutinins of anti- HLA-B7 specificity were shown to be present in two cytotxic anti HLA-Al and three anti- HLA-B13 sera. No cytotoxic anti-HLA-B7 was found in these sera. Cross-reacting antibodies also seem to be frequent among HLA associated haemagglutinins.