Increase in Strength of Red Cell Bg^a Antigen Following Infectious Mononucleosis

The variation in the reactivity of red blood cells (RBC) with HLA haemagglutinins was studied, and the influence of some variable factors evaluted. A comparison of the HLA reactivity of RBC with automated and manual methods, showed the superiority of the Auto- Analyzer technique. The similarity in the nature and behaviour of HLA RBC antigens and the related Chido and Rg antigens is discussed. Inhibition/adsorption experiments were performed, and the results did not support the theory that HLA antigens on RBC originate from serum-HLA-active substance. The strength of the HLA reactivity of RBC from a donor was shown to vary from time to time without evidence of clinical disease.

Section: Resultssupporting

confidence: 94%

Section: Methodssupporting

confidence: 80%

Section: Resultssupporting

confidence: 51%

Section: Methodsmentioning

confidence: 88%

Section: Methodsmentioning

confidence: 99%

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Association between HLA and Red Cell Antigens

Nordhagen¹

1978

Association between HLA and Red Cell Antigens

Nordhagen¹

1978

The variation in the reactivity of red blood cells (RBC) with HLA haemagglutinins was studied, and the influence of some variable factors evaluated. A comparison of the HLA reactivity of RBC with automated and manual methods, showed the superiority of the Auto-Analyzer technique. The similarity in the nature and behaviour of HLA RBC antigens and the related Chido and Rg antigens is discussed. Inhibition/adsorption experiments were performed, and the results did not support the theory that HLA antigens on RBC originate from serum-HLA-active substance. The strength of the HLA reactivity of RBC from a donor was shown to vary from time to time without evidence of clinical disease.

HLA Class I (Bg) Antigens on Red Cells of SLE Patients: A Serological Study with Polyclonal and Monoclonal Antibodies

Giles¹,

Darke²,

Rowe³

et al. 1989

The enhanced HLA class I (Bg) on red blood cells (RBC) of many patients with systemic lupus erythematosus has allowed a significant correlation to be made between their HLA-B types and haemagglutination reactivity with lymphocytotoxic anti-HLA-B sera stimulated by pregnancy alone. Therefore the class I expression on these RBC relates to classical, rather than non-classical, class I gene products. Studies of class I expression on RBC by means of monoclonal antibodies (MAb) to epitopes on the heavy polypeptide chain and β2-microglobulin (β2m) have suggested that the complete extracellular structure is present. The specific effect of chloroquine in ‘stripping HLA’ from RBC had been assumed to support the concept that HLA class I was adsorbed from plasma. However, from our data, we conclude that HLA class I is an intrinsic membrane component. We suggest that the action of chloroquine is to remove β2m alone, which prevents normal class I expression and also results in conformational changes to the class I heavy chain, but that it is not capable of removing the membrane-bound heavy chain.