HLA Class I (Bg) Antigens on Red Cells of SLE Patients:
A Serological Study with Polyclonal and Monoclonal Antibodies

Giles, Carolyn M.; Darke, C.; Rowe, G. P.; Botto, Marina

doi:10.1159/000460975

Cited by 4 publications

(8 citation statements)

References 27 publications

(41 reference statements)

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“…The uptake of W6/32, BBM1, and TP25 by SLE RBCs was confirmed but also showed clearly that chloroquine reduced the reactivity of TP25 much less than W6/32 (Table 2). 16 Again the reactivity for MoAbs W6/32 and BBM1 was partially restored by incubation with ß2-M. 16 We conclude that flow cytometry corroborated the hemagglutination data by a different, more sensitive technique and demonstrated the difference in specificity of MoAbs W6/32 and TP25. Thus, we see that SLE patients have higher numbers of HLA molecules on their RBCs, but this does not correlate with raised levels in their plasma.…”

Section: Flow Cytometry Hemagglutinationmentioning

confidence: 66%

“…Treatment of RBCs with chloroquine removed the reactivity for MoAbs W6/32 and BBMl completely, but residual reactivity with TP2 5 was demonstrated by absorption tests. 16 Clearly, MoAb W6/32 was unable to react with the class I epitope in the absence of the complete HLA complex.…”

Section: Removal and Restoration Of Class I Activity On Sle Rbcsmentioning

confidence: 98%

“…By selecting cytotoxic antisera from women sensitized only by their husbands of known HLA-type during pregnancy, the cross-reactivity in cytotoxicity and hemagglutination tests was reduced,13-16 and it was possible to correlate the known specificity of each antiserum with the HLA-type of RBCs with enhanced expression. 16 Nevertheless, the use of MoAbs-notably W6/32, which reacts with an epitope on the a3 domain-not only demonstrated directly that HLA determinants were present on RBCs, but they also allowed the study of HLA, in a broad sense, that was quite independent of the particular HLA type of each sample. MoAbs have been used in radioimmunoassay,9 flow cytometry,9,16,17 and hemagglutination tests.…”

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confidence: 99%

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Human leukocyte antigens (HLA) class I (Bg) on red cells studied with monoclonal antibodies

Giles

1990

Immunohematology

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Monoclonal antibodies, capable of detecting monomorphic epitopes on HLA class I polypeptides and beta-microglobulin (ß2-M), have been used by a variety of techniques to ascertain the type of structure detected on red blood cells (RBCs). Hemgglutinatlon with class I monoclonal antibodies confirmed the reported relationship between Bg blood groups and HLA. It also established that the expression of HLA on RBCs which do not have nuclei is not normally strong, hut may be enhanced in patients, notably those with systemic lupus erythematosus (SLE). Estimates of the number of class I molecules on mature RBCs by a radioligand-binding assay have confirmed that all HLA-B7 (Bga) individuals have higher numbers but that SLE patients usually have the most (124/RBC). Class I polypeptides were not elevated in the plasm of SLE patients and all RBCs lost molecules on aging in the circulation. These two facts suggest that HLA on RBCs is not acquired from plasma. when RBCs from SLE patients were immunoblotted with monoclonal antibodies, a complete 45 kDa intrinsic transmembrane heavy chain of HLA class I and a light chain of 11 kDa (ß2-M) were detected. Chloroquine treatment and acid elution of RBCs did not remove HLA class I hut only ß2-M, As most antibodies recognize epitopes that depend on close association of dass I with ß2-M, the lost reactivity of treated RBCs may be understood.

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Section: Flow Cytometry Hemagglutinationmentioning

confidence: 66%

Section: Removal and Restoration Of Class I Activity On Sle Rbcsmentioning

confidence: 98%

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confidence: 99%

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Human leukocyte antigens (HLA) class I (Bg) on red cells studied with monoclonal antibodies

Giles

1990

Immunohematology

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“…In addition, new polymorphic antigens, like the recently defined MER-2 antigen [172], may yet be described, although the fact that MER-2 was the first new polymorphic antigen described since 1970 suggests that such discoveries will be relatively few. Finally, mention should be made of three other molecules found at the red cell surface: the glycolipids which express Lewis antigens and are acquired from plasma [reviewed in 21, the fourth component of complement (C4), which is also acquired from plasma and binds to the red cell giving rise to the Ch and Rg antigens [173], and HLA-Class I molecules [174] which are present in very low levels [40-550 (mean 132) molecules/cell; Botto, pers. commun.]…”

Section: Discussionmentioning

confidence: 99%

Blood Group‐Active Surface Molecules of the Human Red Blood Cell

Anstee

1990

Vox Sanguinis

119

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Abstract. The surface of the human red blood cell is dominated by a small number of abundant blood group active proteins. The major proteins are the anion transport protein (band 3) which has AB(H) activity, and Glycophorin A which has MN activity. Band 3 and Glycophorin A are of equal abundance in the normal red cell membrane (approximately lo6 copies of each) and the two proteins may associate together as a complex. The glucose transporter (band 4.5) has AB(H) activity and there are about 5 x lo5 copiedred cell. Several polypeptides associate together to form the Rh complex. The major components of this complex (abundance 1-2 x lo5 copieshed cell) are polypeptides of M, 30,000, polypeptides of MI 45,000-100,000 and Glycophorin B. The antigens of the Rh blood group system appear to be associated with the polypeptides of M, 30,000 and those of M, 45,000-100,000 (the latter also express AB(H) activity). Glycophorin B expresses the blood group 'N' antigen and the Ss antigens. Glycophorins C and D carry the Gerbich antigens and, together, these polypeptides comprise approximately lo5 copiedred cell. The complete protein sequence of all the above-mentioned proteins is known, except for the MI 30,000 and M, 45,000-100,000 polypeptides of the Rh complex for which only partial sequences are available, and Glycophorin D, the sequence of which can be inferred from that of Glycophorin C . Several of the minor blood group active proteins at the red cell surface (abundance 4 . 2 x 104/red cell) have been the subject of recent studies. The polypeptide expressing Cromer-related blood group antigens has been identified as decay-accelerating factor and that carrying the Ina/Inb antigens as CD44. The protein sequence of both of these proteins has been deduced form nucleotide sequencing. The polypeptides expressing Kell antigens, Lutheran antigens, Fy antigens, and LW antigens have also been identified and partially characterised.

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“…While the expression of HLA class I (Bg) on erythrocytes from normal donors has been recognised for some time, its enhancement was also associated with patient groups [l, 6, 81. Recent evidence strongly suggests that erythroid class I is not acquired from plasma but represents an intrinsic membrane molecule [2,11,131. The class I haemagglutination results reflect a numerical increase in these molecules on erythrocytes.…”

Section: Discussionmentioning

confidence: 99%

Erythroid HLA Class I Expression in 300 Patients with Haematological, Renal and Rheumatological Disorders

Ranasinghe¹,

Giles²,

Pusey³

et al. 1990

Vox Sanguinis

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The expression of HLA class I was assessed on erythrocytes by haemagglutination with monoclonal antibodies to monomorphic epitopes on the heavy and light (ß2-microglobulin) chains. Previously, enhancement of HLA class I expression was observed on erythrocytes of many patients with systemic lupus erythematosus (SLE) and chronic lymphatic leukaemia (CLL), and we have now tested erythrocytes from patients (and 130 normal controls) with other auto-immune diseases and renal and haematological disorders. The striking enhancement in patients with SLE and CLL was confirmed. A significant increase in expression was also observed in aplastic anaemia patients following bone marrow transplantation and in renal patients with primary glomerulonephritis who had received a transplant. No class I was expressed by erythrocytes from many patients with inherited haemoglobinopathies and high reticulocyte counts, which suggests that the enhancement in SLE patients cannot be accounted for by immature or young erythrocyte populations. The distribution of HLA-A and -B types in the patients with enhanced class I expression did not relate to those antigens previously detected more frequently on erythrocytes, B7(Bg(a)), B17(Bg(b)), A28(Bgc), B8 or A10, and the enhancement was not associated with any particular HLA types.

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HLA Class I (Bg) Antigens on Red Cells of SLE Patients: A Serological Study with Polyclonal and Monoclonal Antibodies

Cited by 4 publications

References 27 publications

Human leukocyte antigens (HLA) class I (Bg) on red cells studied with monoclonal antibodies

Human leukocyte antigens (HLA) class I (Bg) on red cells studied with monoclonal antibodies

Blood Group‐Active Surface Molecules of the Human Red Blood Cell

Erythroid HLA Class I Expression in 300 Patients with Haematological, Renal and Rheumatological Disorders

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