The Reaction of Formaldehyde with Proteins

Fraenkel‐Conrat, H.; Cooper, Mitzi; Olcott, Henry Steel

doi:10.1021/ja01222a023

Cited by 165 publications

(104 citation statements)

References 44 publications

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“…Fraenkel-Conrat and Olcott [11]. A sample of 10 g of YE was dispersed in 1 dm 3 of methyl alcohol containing HCl (0.1 mol dm -3 ) as a catalyst and mechanically stirred at room temperature.…”

Section: Methylated Yeast Was Prepared According To the Methods Reportmentioning

confidence: 99%

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Biosorption of chromium(VI) and arsenic(V) onto methylated yeast biomass

Seki

Suzuki

Maruyama

2005

Journal of Colloid and Interface Science

135

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Yeast biomass was methylated in a 0.1 M HCl methyl alcohol solution at room temperature and the methylated yeast (MeYE) was applied to the adsorptive separation of Cr(VI) and As(V) anions from aqueous solutions.At near neutral pH, while Cr(VI) and As(V) anions were scarcely adsorbed onto unmethylated yeast biomass, their adsorbed amounts increased with increasing methylation degree. The amount of Cr(VI) adsorbed onto MeYE was almost constant at pH4 -6 and decreased with increasing pH above pH 6. The adsorbed amount of As(V) onto MeYE was rather lower than that of Cr(VI) and it had a peak at about pH 7. A metal-binding model was used to describe the adsorption characteristics of Cr(VI) and As(V) onto MeYE.The results showed that MeYE has two different types of adsorption sites.The saturated adsorbed amount of Cr(VI) and As(V) onto MeYE having methylation degree 0.94 was 0.55 mmol g -1 .

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Section: Methylated Yeast Was Prepared According To the Methods Reportmentioning

confidence: 99%

“…The acid-catalyzed methylation reaction is a specific one involving only the carboxylic groups [11]. Therefore, the proton adsorption isotherm of MeYE can be expressed by the following equation: …”

Section: Proton Binding Sites Of Meyementioning

confidence: 99%

Biosorption of chromium(VI) and arsenic(V) onto methylated yeast biomass

Seki

Suzuki

Maruyama

2005

Journal of Colloid and Interface Science

135

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“…Fraenkel-Conrat and Olcott (18). A 10 g of YE was dispersed in a 1 dm 3 of methyl alcohol containing HCl (0.1 mol dm -3 ) as a catalyst and mechanically stirred at room temperature.…”

Section: Methylated Yeast Was Prepared According To the Methods Reportmentioning

confidence: 99%

“…Fraenkel-Conrat and Olcott (18) reported that the carboxylic groups of proteins were readily methylated at room temperature in methyl alcohol containing small amounts of mineral acid (0.02 to 0.1 mol dm -3 ). The acidcatalyzed reaction of proteins with methanol was a specific one involving only the carboxylic groups; amino, phenolic, thiol, and indole groups and peptide and amide bonds were unaffected.…”

Section: Introductionmentioning

confidence: 99%

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Adsorption of egg albumin onto methylated yeast biomass

Seki

Suzuki

Maruyama

2004

Journal of Colloid and Interface Science

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A new biosorbent, methylated yeast (MeYE), was prepared for the adsorptive separation of proteins from aqueous solutions. Yeast was methylated in a 0.1 M HCl methyl alcohol solution at room temperature. About 80% of the carboxylic groups of yeast could be methylated within 9 h. The adsorption of egg albumin to MeYE was studied to evaluate the protein adsorption ability of MeYE. At near neutral pH, egg albumin was scarcely adsorbed to unmethylated yeast and the adsorption amount of egg albumin increased with increasing the methylation degree. The adsorption amount of egg albumin to MeYE increased with increasing pH from 4 to 7 and steeply decreased above pH 7. The Langmuir isotherm was applied to determine the apparent adsorption constant and the saturated adsorption amount of egg albumin to MeYE. Both the apparent adsorption constant and the saturated adsorption amount increased with the degree of methylation. The saturated adsorption amount of egg albumin to MeYE having methylation degree 77% was 8.41×10 -6 mol g -1 or 0.378 g g -1 at near neutral pH.

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Viscoelastic behavior of formaldehyde and basic chromium sulfate-crosslinked collagen

Usha

Subramanian

Ramasami

1999

J. Appl. Polym. Sci.

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Rat tail tendon (RTT) collagen has been crosslinked with 1% basic chromium sulfate (BCS) at pH 3.2, and 2, 4, 8, and 10% formaldehyde (HCHO) at pH 5 and pH 8. The viscoelastic behavior (such as stress relaxation behavior for BCS and HCHO-tanned RTT) has been studied in water and 6M urea at different temperatures. The total rate of relaxation has been divided into fast and slow components, and computed using the two-term model and nonlinear least-squares fit. The rate of relaxation for crosslinked RTT is less than the native one. Activation energy at absolute zero has been computed using k ϭ AT m e ϪE0/RT and nonlinear least-squares fit. The activation energy increases for crosslinked RTT than the native one. This is consistent with the observed rate constant values. This may be due to the additional stability imparted to RTT collagen by coordinate covalent and covalent crosslinks through BCS and HCHO, respectively.

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The Reaction of Formaldehyde with Proteins

Cited by 165 publications

References 44 publications

Biosorption of chromium(VI) and arsenic(V) onto methylated yeast biomass

Biosorption of chromium(VI) and arsenic(V) onto methylated yeast biomass

Adsorption of egg albumin onto methylated yeast biomass

Viscoelastic behavior of formaldehyde and basic chromium sulfate-crosslinked collagen

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