Adsorption of egg albumin onto methylated yeast biomass

Abstract

A new biosorbent, methylated yeast (MeYE), was prepared for the adsorptive separation of proteins from aqueous solutions. Yeast was methylated in a 0.1 M HCl methyl alcohol solution at room temperature. About 80% of the carboxylic groups of yeast could be methylated within 9 h. The adsorption of egg albumin to MeYE was studied to evaluate the protein adsorption ability of MeYE. At near neutral pH, egg albumin was scarcely adsorbed to unmethylated yeast and the adsorption amount of egg albumin increased with increasing the methylation degree. The adsorption amount of egg albumin to MeYE increased with increasing pH from 4 to 7 and steeply decreased above pH 7. The Langmuir isotherm was applied to determine the apparent adsorption constant and the saturated adsorption amount of egg albumin to MeYE. Both the apparent adsorption constant and the saturated adsorption amount increased with the degree of methylation. The saturated adsorption amount of egg albumin to MeYE having methylation degree 77% was 8.41×10 -6 mol g -1 or 0.378 g g -1 at near neutral pH.

Biosorption of chromium(VI) and arsenic(V) onto methylated yeast biomass

“…Therefore, it is not expected that these metals can adsorb onto microorganisms at near-neutral pH. In our previous study [10], we prepared a new biosorbent, methylated yeast (MeYE), for the adsorptive separation of negatively charged proteins. At near-neutral pH, egg albumin which has negative charge was scarcely adsorbed onto unmethylated yeast, but its adsorbed amount ramarkably increased with the increase of methylation degree.…”

Adsorption Characteristics and Optimal Dosage of Flocculants in the Solid Separation of Suspensions

“…Because θ a + θ s = 1, we obtain θ a as As a result, the amount of flocculant adsorbed on the particles, X (g/g of particles), is where γ (g/g of particles) represents the saturated amount of adsorbed flocculant on the particles. As seen in eq 4, the adsorption equilibrium can be expressed by a Langmuir isotherm. − …”

“…In the current study, we propose a flocculation model that takes into account the bridging reaction between adsorbed polymer flocculant surface on a particle and the bare surface on another particle. To verify the validity of the model, adsorption and flocculation experiments were conducted for quartz suspensions with two types of polymer flocculants that were developed in our previous studies. − …”

“…To verify the validity of the model, adsorption and flocculation experiments were conducted for quartz suspensions with two types of polymer flocculants that were developed in our previous studies. [10][11][12][13][14][15][16][17] Flocculation Model 1. Adsorption of Flocculant on Particles.…”

Adsorption of Lysozyme Using Citric Acid Modified Waste Beer Yeast

“…Many studies have attempted to introduce some functional groups as metal scavenger through some derivatizations, such as poly (amic acid) modified biomass for lead and cadmium adsorption (1); ethylenediamine modified peanut shells for mercury and cadmium adsorption (2); succinate-bonded cellulose for cadmium removal (3). However, little is known about how to use modified waste beer yeast to adsorb proteins (4), especially lysozyme (LSZ).…”