The qualitative and quantitative determination of quinolones of first and second generation by capillary electrophoresis

Fierens, Colette; Hillaert, S; Bossche, W. Van den

doi:10.1016/s0731-7085(99)00282-4

Cited by 102 publications

(60 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…3 CE proved to be an important tool in the analysis of FQ derivatives but the large majority of the developed methods describe separation of FQs from different generations having different physicochemical characteristics. [4][5][6][7][8] Ciprofloxacin (CIP), norfloxacin (NOR) and ofloxacin (OFL) (three 2 nd generation FQs) are by far the important and widely used FQs. CIP and NOR have almost similar structural characteristics, the substituent at the nitrogen atom from the pyridin-carboxilic ring (cyclopropyl and ethyl side chain, respectively) being the only difference between the two, while OFL is a tricyclic derivative and has a methyl substituent on the piperazine ring ( Figure 1).…”

Section: Introductionmentioning

confidence: 99%

“…9 Some CE methods have been developed for the determination of CIP, NOR and OFL, but their simultaneous determination is challenging because of their similar electrophoretic mobilities. 6,7 The aim of our study was to prove the applicability of the CE method for the simultaneous separation of the studied FQs by developing a rapid, simple and sensitive procedure for their simultaneous determination and also to conduct a systematic study of the analytical conditions (i.e., the effects of buffer concentration, modifier concentration and buffer pH) in order to improve the separation.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Simultaneous separation of ciprofloxacin, norfloxacin and ofloxacin by micellar electrokinetic chromatography

Hancu¹,

Rusu²,

Simon³

et al. 2012

J. Braz. Chem. Soc.

View full text Add to dashboard Cite

Este trabalho descreve o desenvolvimento de um método simples de eletroforese capilar (EC) para determinação simultânea dos três derivados mais frequentemente usados da fluoroquinolona: ciprofloxacina, norfloxacina e ofloxacina. Devido às características estruturais e físico-químicas muito semelhantes, ciprofloxacina e norfloxacina não podem ser separadas pelo método clássico de eletroforese capilar de zona (CZE). Suas separações podem ser resolvidas usando cromatografia eletrocinética micelar (MEKC), adicionando um surfactante aniônico à solução-tampão. A eficiência da separação pode ser intensificada através da otimização de parâmetros analíticos. Um método rápido e confiável foi desenvolvido, e os melhores resultados foram obtidos usando uma solução-tampão contendo tetraborato de sódio 25 mmol L -1 , dodecilsulfato de sódio 100 mmol L -1 e metanol 5% como modificador orgânico.This work describes the development of a simple capillary electrophoresis (CE) method for simultaneous determination of the three most frequently used fluoroquinolone derivatives: ciprofloxacin, norfloxacin and ofloxacin. Due to very similar structural and physicochemical characteristics, ciprofloxacin and norfloxacin cannot be separated by the classic method of capillary zone electrophoresis (CZE). Their separations can be solved using micellar electrokinetic chromatography (MEKC), by adding an anionic surfactant to the buffer solution. The efficiency of the separation can be enhanced through the optimization of analytical parameters. A fast reliable method was developed, and the best results were obtained using a buffer solution containing 25 mmol L -1 sodium tetraborate, 100 mmol L -1 sodium dodecyl sulphate and 5% methanol as organic modifier.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Simultaneous separation of ciprofloxacin, norfloxacin and ofloxacin by micellar electrokinetic chromatography

Hancu¹,

Rusu²,

Simon³

et al. 2012

J. Braz. Chem. Soc.

View full text Add to dashboard Cite

show abstract

“…6,7 It is determined by capillary electrophoresis, [8][9][10] polarography, 11 voltammetry, 12 high performance liquid chromatography (HPLC), 13 spectrofluorimetry, 14,15 and chemiluminescence. 16,17 Among these techniques CL method combined with flow injection is well known as the powerful analytical technique that provides the advantages of simple equipment, high sensitivity detection and producing low background signals for the emission of CL coming from the chemical reaction. However, a few flow injection CL methods were reported for the determination of NFLX in pharmaceutical and biological samples with different reaction systems such as, Au nanoparticles (NPs)-NFLX-Ce(IV)-Na 2 SO 3 system, 18 [Ag(HIO 6 21 CL reaction of sulphite with Ce(IV) sensitized by NFLX, 22 micelle-sensitized Ce(IV)-Na 2 S 2 O 3 -NFLX system, 23 FQs-soluble manganese (IV)-sulphite system, 24 FQs-Ru(bipy) 3…”

Section: Introductionmentioning

confidence: 99%

A Metal Enhanced Flow-Injection Chemiluminescence Method for the Rapid Determination of Norfloxacin in Pharmaceutical Formulations and Serum Sample

Kamruzzaman¹,

Ferdous²,

Alam³

et al. 2011

Bulletin of the Korean Chemical Society

View full text Add to dashboard Cite

A simple and highly sensitive chemiluminescence method to determine norfloxacin (NFLX) has been proposed by measuring the chemiluminescence (CL) intensities using a flow injection (FI) system. The CL intensity of the luminol-H2O2 system is strongly enhanced by the addition of Cu (II) in alkaline condition. The CL intensity is substantially increased after the injection of NFLX into the luminol-H2O2-Cu (II) system. The enhancement effect is attributed to a catalytic effect of Cu (II) due to the interaction with NFLX which forms a complex with the catalyst. Under the optimal conditions, the sensitizing effect of the CL intensity is proportional to the concentration of NFLX in the range of 1.5 × 10 -9 -5.9 × 10 -7 mol L -1 (r = 0.9994) with a detection limit (3σ) of 2.98 × 10 -10 mol L -1 . The proposed method had good reproducibility with the relative standard deviation (RSD, n = 5) of 1.6% for 1 × 10 -7 mol L −1 of NFLX. The possible reaction mechanism of the CL reaction is also discussed. This method has been successfully applied for the determination of trace amount of NFLX in pharmaceutical preparations and serum samples.

show abstract

“…After that, Sun and Wu, 9 achieved the separation of 7 FQs using sodium borate/ sodium dihydrogen phosphate/ sodium cholate/ acetonitrile within 8.5 min of electrolyte running. Finally, Fierens et al 10 performed partial separation of 10 quinolones and FQs at about 14 min using sodium phosphate buffer (pH ≈ 7). Additionally, the reviews published by Flurer describing recent advances in the analysis of antibiotics by CE, among which the FQs, presented an overview about the determination of this kind of pharmaceutical in different matrixes.…”

Section: Introductionmentioning

confidence: 99%

“…This methodology, in comparison with others previously described in the literature for the analysis of FQs pharmaceutical formulations, presents the analysis of more recent FQs such as GTLFX and MFLX, shorter analysis time and simpler reagents in the background electrolyte. [8][9][10] Table 1 shows the excipients present in each tablet analyzed. The presence of these makes the use of a selective method necessary.…”

Section: Introductionmentioning

confidence: 99%

Validation of a capillary zone electrophoresis method for the determination of ciprofloxacin, gatifloxacin, moxifloxacin and ofloxacin in pharmaceutical formulations

Faria¹,

Souza²,

Oliveira³

2008

J. Braz. Chem. Soc.

View full text Add to dashboard Cite

Um método alternativo por eletroforese capilar de zona (CZE) para determinação de ciprofloxacina (CPFLX), gatifloxacina (GTFLX), moxifloxacina (MFLX) e ofloxacina (OFLX) foi validado. O sistema de eletrólito utilizado consistiu da mistura de 25 mmol L -1 de TRIS/ HCl e 15 mmol L -1 de tetraborato de sódio em meio aquoso resultando em pH 8,87. A análise foi realizada sob detecção direta por UV em 282 nm com tempo de análise de 3 min. Os parâmetros analíticos de validação avaliados foram: linearidade (r > 0,998), seletividade (comparação entre a inclinação da curva de calibração de padronização externa e curva de calibração de adição de padrão), repetitividade em área para amostras (RSD %: < 3,94% para CPFLX, < 3,87% para GTFLX, 1,30% para MFLX e < 1,88% para OFLX), precisão intermediária em área para amostras (RSD%: < 3,59% para CPFLX, < 3,09% para GTFLX, 2,67% para MFLX e < 2,25% para OFLX), exatidão (média da faixa de recuperação: 101,2% para CPFLX, 101,0% para GTFLX, 101,3% para MFLX e 99,9% para OFLX), limite de detecção (mg L -1 : 2,72 para CPFLX, 1,92 para GTFLX, 0,795 para MFLX e 1,05 para OFLX), limite de quantificação (mg L -1 : 9,06 para CPFLX, 6,40 para GTFLX, 2,65 para MFLX e 3,50 para OFLX) e robustez. Devido à simplicidade, seletividade, precisão, exatidão e rapidez, o método pode ser uma alternativa interessante para auxiliar o controle da qualidade dessas drogas na indústria farmacêutica.An alternative capillary zone electrophoresis (CZE) method for the determination of ciprofloxacin (CPFLX), gatifloxacin (GTFLX), moxifloxacin (MFLX) and ofloxacin (OFLX) through a simple aqueous electrolyte system consisting of 25 mmol L -1 of TRIS/ hydrochloride and 15 mmol L -1 of sodium tetraborate buffer mixture (pH 8.87) using direct UV detection at 282 nm within 3 min was validated. The analytical parameters of validation evaluated were: linearity (r > 0.998), selectivity (comparison between slope of the calibration curve of external standard and calibration curve of standard addition), repeatability in area for sample (RSD%: < 3.94% for CPFLX, < 3.87% for GTFLX, 1.30% for MFLX and < 1.88% for OFLX), intermediate precision in area for sample (RSD%: < 3.59% for CPFLX, < 3.09% for GTFLX, 2.67% for MFLX and < 2.25% for OFLX), accuracy (mean of recovery range: 101.2% for CPFLX, 101.0% for GTFLX, 101.3% for MFLX and 99.9% for OFLX), limit of detection (mg L -1 : 2.72 for CPFLX, 1.92 for GTFLX, 0.795 for MFLX and 1.05 for OFLX), limit of quantification (mg L -1 : 9.06 for CPFLX, 6.40 for GTFLX, 2.65 for MFLX and 3.50 for OFLX) and robustness. Due to its simplicity, selectivity, precision, accuracy and rapidity, the methodology can be an interesting alternative for quality assurance in the pharmaceutical industry of these drugs.

show abstract

The qualitative and quantitative determination of quinolones of first and second generation by capillary electrophoresis

Cited by 102 publications

References 12 publications

Simultaneous separation of ciprofloxacin, norfloxacin and ofloxacin by micellar electrokinetic chromatography

Simultaneous separation of ciprofloxacin, norfloxacin and ofloxacin by micellar electrokinetic chromatography

A Metal Enhanced Flow-Injection Chemiluminescence Method for the Rapid Determination of Norfloxacin in Pharmaceutical Formulations and Serum Sample

Validation of a capillary zone electrophoresis method for the determination of ciprofloxacin, gatifloxacin, moxifloxacin and ofloxacin in pharmaceutical formulations

Contact Info

Product

Resources

About