The Putative Exchange Factor Gef3p Interacts with Rho3p GTPase and the Septin Ring during Cytokinesis in Fission Yeast

Muñoz, Sofía; Manjón, Elvira; Sánchez, Yolanda

doi:10.1074/jbc.m114.548792

Cited by 18 publications

(27 citation statements)

References 69 publications

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“…Rho4 GTPase is also required for glucanase secretion (Santos et al ., ), likely through exocyst regulation (Pérez et al ., ). This GTPase is activated by Gef3, which interacts with and is localized by the septins (Muñoz et al ., ; Wang et al ., ). In this way, a precise spatio‐temporal regulation of glucanase secretion preserves cell integrity.…”

Section: Introductionmentioning

confidence: 99%

Overview of fission yeast septation

Pérez

Cortés

Martín‐García

et al. 2016

Cellular Microbiology

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Summary Cytokinesis is the final process of the vegetative cycle, which divides a cell into two independent daughter cells once mitosis is completed. In fungi, as in animal cells, cytokinesis requires the formation of a cleavage furrow originated by constriction of an actomyosin ring which is connected to the plasma membrane and causes its invagination. Additionally, because fungal cells have a polysaccharide cell wall outside the plasma membrane, cytokinesis requires the formation of a septum coincident with the membrane ingression. Fission yeast Schizosaccharomyces pombe is a unicellular, rod‐shaped fungus that has become a popular model organism for the study of actomyosin ring formation and constriction during cell division. Here we review the current knowledge of the septation and separation processes in this fungus, as well as recent advances in understanding the functional interaction between the transmembrane enzymes that build the septum and the actomyosin ring proteins.

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Section: Introductionmentioning

confidence: 99%

Overview of fission yeast septation

Pérez

Cortés

Martín‐García

et al. 2016

Cellular Microbiology

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“…While the authors report that addition of the drug aculeacin A (blocks glucan synthesis) does not lead to ring sliding, Bgs1 is resistant to this drug and thus one cannot conclude if the Bgs1 enzymatic activity is necessary for anchoring the ring (Castro et al, 1995;Martins et al, 2011). Bgs1 has been reported to cooperate with Paxillin Pxl1 and Cdc15 to maintain ring integrity and to Martin-Cuadrado et al (2003), Wang et al (2002), Garcia et al (2005), Sipiczki et al (2014), Martin-Cuadrado et al (2008), Wang et al (2003;2015), Perez et al (2015), Santos et al (2005), Munoz et al (2014), Villalobos-Duno et al (2013), Nakano et al (2003) promote septum synthesis (Cortes et al, 2015). Similar to cdc15 and bgs1 mutants, pxl1 mutants also show ring sliding defects (Cortes et al, 2015).…”

Section: Septum-driven Cytokinesis In Fission Yeastmentioning

confidence: 99%

“…Septin protein complexes are also required for the delivery of glucanases to the division site. This includes the anillin homolog Mid2 and the septin proteins Spn1-4 (Tasto et al, 2003;An et al, 2004;Martin-Cuadrado et al, 2005;Sipiczki, 2007;Roncero and Sanchez, 2010;Wu et al, 2010;Munoz et al, 2014;Perez et al, 2015;Wang et al, 2015;Zheng et al, 2018). Mid2 organizes the septin ring that forms on either side of the septum barrier (Tasto et al, 2003).…”

Section: Septum Digestion and Cell Separationmentioning

confidence: 99%

Coordinating septum formation and the actomyosin ring during cytokinesis in Schizosaccharomyces pombe

Hercyk

Onwubiko

Das

2019

Molecular Microbiology

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Summary During cytokinesis, animal and fungal cells form a membrane furrow via actomyosin ring constriction. Our understanding of actomyosin ring‐driven cytokinesis stems extensively from the fission yeast model system. However, unlike animal cells, actomyosin ring constriction occurs simultaneously with septum formation in fungi. While the formation of an actomyosin ring is essential for cytokinesis in fission yeast, proper furrow formation also requires septum deposition. The molecular mechanisms of spatiotemporal coordination of septum deposition with actomyosin ring constriction are poorly understood. Although the role of the actomyosin ring as a mechanical structure driving furrow formation is better understood, its role as a spatiotemporal landmark for septum deposition is not widely discussed. Here we review and discuss the recent advances describing how the actomyosin ring spatiotemporally regulates membrane traffic to promote septum‐driven cytokinesis in fission yeast. Finally, we explore emerging questions in cytokinesis, and discuss the role of extracellular matrix during cytokinesis in other organisms.

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“…Rho1p activity is regulated by three GEFs: Rgf1p, Rgf2p, and Rgf3p [8,[27][28][29][30]. Other members of the Rho-GEF family in S. pombe are the Cdc42p-specific GEFs: Scd1p and Gef1p [31,32], and Gef3p specific for Rho4p [33,34]. Gef2p [35,36] and Mug10p have not yet been assigned to any known GTPase (https://www.pombase.org).…”

mentioning

confidence: 99%

The Function of Fission Yeast Rho1-GEFs in the Control of Cell Growth and Division

Edreira¹,

Manjón²,

Sánchez³

2018

Peripheral Membrane Proteins

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Guanine nucleotide exchange factors (GEFs) are directly responsible for the activation of Rho-family GTPases in response to physical and chemical stimuli and ultimately regulate numerous cellular responses such as polarized growth, morphogenesis, and movement. The GEF proteins are characterized by a Dbl-homology (DH) domain that contacts the Rho GTPases, to catalyzing nucleotide exchange, and an associated Pleckstrin homology (PH) domain, which fine-tunes the exchange process by a variety of mechanisms related to the binding of phosphoinositides. Most GEFs are divergent in regions outside the DH/ PH module and contain additional protein-protein or lipid-protein interaction domains that presumably dictate unique cellular functions. Fission yeast Rho1-GEFs act as a link between growth processes and the cell cycle machinery. In this chapter, we focus on the recent leaps in our understanding of how Rho1-GEFs control interphase and cytokinesis in fission yeast. Furthermore, we will go beyond mitosis and highlight the unexpected roles of Rho1-GEFs in the DNA damage response.

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The Putative Exchange Factor Gef3p Interacts with Rho3p GTPase and the Septin Ring during Cytokinesis in Fission Yeast

Cited by 18 publications

References 69 publications

Overview of fission yeast septation

Overview of fission yeast septation

Coordinating septum formation and the actomyosin ring during cytokinesis in Schizosaccharomyces pombe

The Function of Fission Yeast Rho1-GEFs in the Control of Cell Growth and Division

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