Coordinating septum formation and the actomyosin ring during cytokinesis in <i>Schizosaccharomyces pombe</i>

Hercyk, Brian S.; Onwubiko, Udo N; Das, Maitreyi

doi:10.1111/mmi.14387

Cited by 19 publications

(14 citation statements)

References 121 publications

(289 reference statements)

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“…We also identified an additional change in density at cell birth. At the end of cytokinesis, the middle layer of the septum is digested, and daughter cells separate (20, 21). During this 5-10-min period, the septum bulges outward on each side to form the rounded shape of the new end, in a physical process driven by turgor pressure (22).…”

Section: Resultsmentioning

confidence: 99%

Variations of intracellular density during the cell cycle arise from tip-growth regulation in fission yeast

Odermatt

Bostan

Huang

et al. 2020

Preprint

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Intracellular density is a critical parameter that impacts the physical nature of the cytoplasm and has the potential to globally affect cellular processes. How density is regulated during cell growth is poorly understood. Here, using a new quantitative phase imaging method, we show that dry-mass density varies during the cell cycle in the fission yeast Schizosaccharomyces pombe. Density decreased during G2 phase, increased in mitosis and cytokinesis, and rapidly dropped at cell birth. These density variations can be explained mostly by a constant rate of biomass synthesis throughout the cell cycle, coupled to slowdown of volume growth during mitosis and cytokinesis and rapid expansion post-cytokinesis. Arrest at specific cell-cycle stages led to continued increases or decreases in density. Spatially heterogeneous patterns of density suggested a link between tip growth and density regulation. Density differences between daughter cells in cells delayed in cytokinesis resulted in bending of the septum away from the high-density daughter, suggesting that intracellular density correlates with turgor pressure. Our results demonstrate that the systematic variations in density during the cell cycle are determined predominantly by modulation of volume expansion, and reveal functional consequences of intracellular density gradients.

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Section: Resultsmentioning

confidence: 99%

Variations of intracellular density during the cell cycle arise from tip-growth regulation in fission yeast

Odermatt

Bostan

Huang

et al. 2020

Preprint

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“…Insufficient SIN signaling results in improper assembly of the contractile ring and failure of the cytokinesis, generating multinucleated cells, whereas too much SIN signaling uncouples cytokinesis from the rest of the cell cycle [ 49 ]. As such, the cytokinetic events need to be precisely regulated to ensure that they occur in a proper and sequential manner [ 50 ]. Additionally, after the enrichment of the PGC and SAX fractionated samples for proteins involved in regulation of cytokinesis, we also identified multiple novel phosphorylation sites of proteins involved in cell division and cytokinesis ( Table S1 ).…”

Section: Resultsmentioning

confidence: 99%

Label-Free Quantitative Phosphoproteomics of the Fission Yeast Schizosaccharomyces pombe Using Strong Anion Exchange- and Porous Graphitic Carbon-Based Fractionation Strategies

Siváková

Jurcik

Lukáčová

et al. 2021

IJMS

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The phosphorylation of proteins modulates various functions of proteins and plays an important role in the regulation of cell signaling. In recent years, label-free quantitative (LFQ) phosphoproteomics has become a powerful tool to analyze the phosphorylation of proteins within complex samples. Despite the great progress, the studies of protein phosphorylation are still limited in throughput, robustness, and reproducibility, hampering analyses that involve multiple perturbations, such as those needed to follow the dynamics of phosphoproteomes. To address these challenges, we introduce here the LFQ phosphoproteomics workflow that is based on Fe-IMAC phosphopeptide enrichment followed by strong anion exchange (SAX) and porous graphitic carbon (PGC) fractionation strategies. We applied this workflow to analyze the whole-cell phosphoproteome of the fission yeast Schizosaccharomyces pombe. Using this strategy, we identified 8353 phosphosites from which 1274 were newly identified. This provides a significant addition to the S. pombe phosphoproteome. The results of our study highlight that combining of PGC and SAX fractionation strategies substantially increases the robustness and specificity of LFQ phosphoproteomics. Overall, the presented LFQ phosphoproteomics workflow opens the door for studies that would get better insight into the complexity of the protein kinase functions of the fission yeast S. pombe.

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“…Constriction speeds as anaphase concludes, and cytokinesis begins to occur in conjunction with this transition ( Willet et al., 2015 ). Glucan ( S. pombe ) or chitin ( S. cerevisiae ) synthesizing proteins are recruited to the ring ( Santos et al., 2003 ; Hercyk et al., 2019 ). These proteins induce ingression of the septum and membrane invagination, processes which are not only coupled to ring constriction, but also required for proper placement of the ring ( Willet et al., 2015 ).…”

Section: The Most Well Characterized Contractile Rings: S Pombe and S Cerevisiae Modelsmentioning

confidence: 99%

The Ringleaders: Understanding the Apicomplexan Basal Complex Through Comparison to Established Contractile Ring Systems

Morano

Dvorin

2021

Front. Cell. Infect. Microbiol.

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The actomyosin contractile ring is a key feature of eukaryotic cytokinesis, conserved across many eukaryotic kingdoms. Recent research into the cell biology of the divergent eukaryotic clade Apicomplexa has revealed a contractile ring structure required for asexual division in the medically relevant genera Toxoplasma and Plasmodium; however, the structure of the contractile ring, known as the basal complex in these parasites, remains poorly characterized and in the absence of a myosin II homolog, it is unclear how the force required of a cytokinetic contractile ring is generated. Here, we review the literature on the basal complex in Apicomplexans, summarizing what is known about its formation and function, and attempt to provide possible answers to this question and suggest new avenues of study by comparing the Apicomplexan basal complex to well-studied, established cytokinetic contractile rings and their mechanisms in organisms such as S. cerevisiae and D. melanogaster. We also compare the basal complex to structures formed during mitochondrial and plastid division and cytokinetic mechanisms of organisms beyond the Opisthokonts, considering Apicomplexan diversity and divergence.

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Coordinating septum formation and the actomyosin ring during cytokinesis in Schizosaccharomyces pombe

Cited by 19 publications

References 121 publications

Variations of intracellular density during the cell cycle arise from tip-growth regulation in fission yeast

Variations of intracellular density during the cell cycle arise from tip-growth regulation in fission yeast

Label-Free Quantitative Phosphoproteomics of the Fission Yeast Schizosaccharomyces pombe Using Strong Anion Exchange- and Porous Graphitic Carbon-Based Fractionation Strategies

The Ringleaders: Understanding the Apicomplexan Basal Complex Through Comparison to Established Contractile Ring Systems

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