The Function of Fission Yeast Rho1-GEFs in the Control of Cell Growth and Division

Abstract

Guanine nucleotide exchange factors (GEFs) are directly responsible for the activation of Rho-family GTPases in response to physical and chemical stimuli and ultimately regulate numerous cellular responses such as polarized growth, morphogenesis, and movement. The GEF proteins are characterized by a Dbl-homology (DH) domain that contacts the Rho GTPases, to catalyzing nucleotide exchange, and an associated Pleckstrin homology (PH) domain, which fine-tunes the exchange process by a variety of mechanisms related to the binding of phosphoinositides. Most GEFs are divergent in regions outside the DH/ PH module and contain additional protein-protein or lipid-protein interaction domains that presumably dictate unique cellular functions. Fission yeast Rho1-GEFs act as a link between growth processes and the cell cycle machinery. In this chapter, we focus on the recent leaps in our understanding of how Rho1-GEFs control interphase and cytokinesis in fission yeast. Furthermore, we will go beyond mitosis and highlight the unexpected roles of Rho1-GEFs in the DNA damage response.

Regulación de la citoquinesis por Rgf1 y la ruta de integridad

“…Esto indica que no es fácil disponer de herramientas para estudiar la función de Rho1. De hecho, gran parte de lo que sabemos sobre su función se debe al estudio de sus reguladores GEFs y GAPs, que modulan la localización y la actividad de Rho1 en momentos determinado del ciclo (Edreira et al 2018).…”