“…Although the importance of chicken egg as an economical and convenient source of polyclonal antibodies has been well recognised, the widespread use is often considered impractical partly due to the difficulties in isolation of IgY from yolk (Devi et al, 2002;Kuroki, 1999). The conventional techniques such as precipitation (Akita & Nakai, 1993), electrophoresis (Gee, Bate, Thomas, & Rylatt, 2003), and chromatography (Hansen, Scoble, Hanson, & Hoogenraad, 1998;Tini, Jewell, Camenisch, Chilov, & Gassmann, 2002;Verdoliva, Basile, & Fassina, 2000) have been used in the IgY separation and purification process. However, these conventional techniques are often time consuming and low yielding (Verdoliva et al, 2000).…”