The Pseudomonas aeruginosa Mannose Sensitive Hamemagglutination Strain (PA-MSHA) Induces a Th1-Polarizing Phenotype by Promoting Human Dendritic Cells Maturation

Zhang, Yunyan; Wang, Hongtao; Li, Youqiang; Chen, Ke; Ye, Jinmei; Liao, Xiubei; Chen, Yiyang; Wei, Ran

doi:10.1007/s12088-013-0436-6

Cited by 7 publications

(6 citation statements)

References 20 publications

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“…Dendritic cells are the most potent antigen presenting cells that are able to efficiently induce primary T-cell-mediated responses to pathogens, including bacteria. The induction of dendritic cell maturation was confirmed in previous research [13,14]. We found a statistically significant difference in percentage of mature DCs (CD83+CD1a-HLA-DR++) after incubation with Pseudomonas aeruginosa lysates between the strains isolated from the most severely ill patients and from asymptomatic carriers.…”

Section: Discussionsupporting

confidence: 90%

Evaluation of Selected Parameters of the Specific Immune Response against Pseudomonas aeruginosa Strains

Dzik

Aebisher

Olender

et al. 2021

Cells

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The immune response to Pseudomonas aeruginosa strains could be influenced by differences in antibiotic resistance and virulence. At the present time, it is unclear which type of immune responses enables uncontrolled invasion of opportunistic pathogens. The conditional pathogenicity of Pseudomonas aeruginosa served as an inspiration to begin a study on this bacterium. The aim of this study was to gain insight into selected parameters describing immune responses with regards to the adaptable agents of this pathogen. For the analysis of the specific immune response, the potential of Pseudomonas aeruginosa to stimulate lymphocytes, including Th17 lymphocytes, dendritic cells and other components of the adaptive immune response, was examined. The highest percentage of CD83+CD1a-HLA-DR++ cells was found after stimulation with lysates of strains isolated from the patients with severe systemic infection. We found statistically significant differences in percentages of HLA-DR+ PBMCs and MFI of HLA-DR between groups of Pseudomonas aeruginosa strains isolated from the patients with different clinical courses of infection. Our results suggest that the clinical course and outcomes of Pseudomonas aeruginosa infections are not associated with impairment of the specific immune response.

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Section: Discussionsupporting

confidence: 90%

Evaluation of Selected Parameters of the Specific Immune Response against Pseudomonas aeruginosa Strains

Dzik

Aebisher

Olender

et al. 2021

Cells

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“…The higher diagnostic sensitivity at the presence of P. aeruginosa might lie in the following reason. The Pseudomonas aeruginosa mannose sensitive hemagglutination strain (PA-MSHA)-primed dendritic cells (DCs) directed T cell differentiation and primed Th cell to Th1 by an elevated secretion of IFN-γ ( Zhang et al, 2014 ). In conclusion, under various kinds of pathologic status especially in the case of a bacterial infection, the level of GSDMD changed quickly and had a large variation in the amplitude, which improved the diagnostic sensitivity and the ability of an early diagnosis.…”

Section: Discussionmentioning

confidence: 99%

Pleural Fluid GSDMD Is a Novel Biomarker for the Early Differential Diagnosis of Pleural Effusion

Shi

Zhou

et al. 2021

Front. Microbiol.

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ObjectiveGasdermin D (GSDMD), controlling pyroptosis in cells, has multiple physiological functions. The diagnostic role of GSDMD in pleural effusion (PE) remains unknown.MethodsSandwich ELISA kits that we developed were applied to measure the level of GSDMD for 335 patients with a definite cause of PE, including transudative PE, tuberculous pleural effusion (TPE), parapneumonic pleural effusion (PPE), and malignant pleural effusion (MPE). The diagnostic accuracy of Light’s criteria vs. the new marker GSDMD was performed. Clinical follow-up of 40 cases of PPE was conducted and divided into efficacy and non-efficacy groups according to the therapeutic outcome. Nucleated cells (NCs) in PE were isolated and further infected with bacteria to verify the cell source of GSDMD.ResultsThe diagnostic accuracy of GSDMD for the diagnosis of PE were 96% (sensitivity) and 94% (specificity). The receiver operating characteristic (ROC) curve indicated that GSDMD can be an efficient biomarker for the differential diagnosis of transudative PE and other groups (all AUC > 0.973). Noteworthily, the highest AUC belonged to tuberculosis diagnosis of 0.990, and the cut-off value was 18.40 ng/mL. Moreover, the same cut-off value of PPE and MPE was 9.35 ng/mL. The combination of GSDMD, adenosine deaminase (ADA), and lactate dehydrogenase (LDH) will further improve the diagnostic efficiency especially between TPE and PPE (AUC = 0.968). The AUC of GSDMD change at day 4, which could predict the therapeutic effect at an early stage, was 0.945 (P < 0.0001). Interestingly, bacterial infection experiments further confirm that the pleural fluid GSDMD was expressed and secreted mainly by the NCs.ConclusionGSDMD and its combination are candidates as a potentially novel biomarker not only to separate PEs early and effectively, but also monitor disease progression.

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“…P . aeruginosa does not only stimulate TNFα production in epithelial cells [ 14 ], but also induces dendritic cell maturation to the Th1-polaizing phenotype [ 15 ]. Moreover, P .…”

Section: Discussionmentioning

confidence: 99%

Association of Mucosal Organisms with Patterns of Inflammation in Chronic Rhinosinusitis

et al. 2015

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BackgroundChronic rhinosinusitis is a multifactorial process disease in which bacterial infection or colonization may play an important role in the initiation or persistence of inflammatory response. The association between mucosal bacteria presence and inflammatory patterns has only been partially explored.ObjectiveTo demonstrate specific mucosal microorganisms possible association with inflammatory patterns.MethodsWe collected nasal polyps or sinus tissues from a clinical selection of six patient groups with defined sinus disease using tissue biomarkers. In the tissues, we detected bacteria using peptide nucleic acid fluorescence in situ hybridization (PNA-FISH).ResultsAfter reviewing a total of 115 samples (15–20 samples per group), the mucosal presence of Staphylococcus aureus was correlated with IL-5 and SE-IgE positive chronic rhinosinusitis with nasal polyps and nasal polyps from cystic fibrosis patients. Chronic rhinosinusitis without nasal polyps with TNFα >20 pg/ml was associated with the mucosal presence of Pseudomonas aeruginosa.ConclusionThis study identifies the relationship between intramucosal microbes and inflammatory patterns, suggesting that bacteria may affect the type of inflammation in chronic rhinosinusitis. Additional investigation is needed to further identify the nature of the relationship.

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The Pseudomonas aeruginosa Mannose Sensitive Hamemagglutination Strain (PA-MSHA) Induces a Th1-Polarizing Phenotype by Promoting Human Dendritic Cells Maturation

Cited by 7 publications

References 20 publications

Evaluation of Selected Parameters of the Specific Immune Response against Pseudomonas aeruginosa Strains

Evaluation of Selected Parameters of the Specific Immune Response against Pseudomonas aeruginosa Strains

Pleural Fluid GSDMD Is a Novel Biomarker for the Early Differential Diagnosis of Pleural Effusion

Association of Mucosal Organisms with Patterns of Inflammation in Chronic Rhinosinusitis

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