Evaluation of Selected Parameters of the Specific Immune Response against Pseudomonas aeruginosa Strains

Abstract

The immune response to Pseudomonas aeruginosa strains could be influenced by differences in antibiotic resistance and virulence. At the present time, it is unclear which type of immune responses enables uncontrolled invasion of opportunistic pathogens. The conditional pathogenicity of Pseudomonas aeruginosa served as an inspiration to begin a study on this bacterium. The aim of this study was to gain insight into selected parameters describing immune responses with regards to the adaptable agents of this pathogen. For the analysis of the specific immune response, the potential of Pseudomonas aeruginosa to stimulate lymphocytes, including Th17 lymphocytes, dendritic cells and other components of the adaptive immune response, was examined. The highest percentage of CD83+CD1a-HLA-DR++ cells was found after stimulation with lysates of strains isolated from the patients with severe systemic infection. We found statistically significant differences in percentages of HLA-DR+ PBMCs and MFI of HLA-DR between groups of Pseudomonas aeruginosa strains isolated from the patients with different clinical courses of infection. Our results suggest that the clinical course and outcomes of Pseudomonas aeruginosa infections are not associated with impairment of the specific immune response.

An integrated multi-omics analysis of identifies distinct molecular characteristics in pulmonary infections of Pseudomonas aeruginosa

“…P . aeruginosa can not only effectively counteract immune mechanisms but may also possess a variety of intrinsic and possibly acquired antibiotic resistance traits [ 1 , 2 ]. With widespread environmental occurrence and strong medical relevance, P .…”

“…Pseudomonas aeruginosa (P. aeruginosa) is a gram-negative pathogenic aerobic bacterium that can metabolize anaerobically under certain conditions and has a high degree of virulence modification and immune bypassing ability. P. aeruginosa can not only effectively counteract immune mechanisms but may also possess a variety of intrinsic and possibly acquired antibiotic resistance traits [1,2]. With widespread environmental occurrence and strong medical relevance, P. aeruginosa is a common cause of hospital respiratory infections and morbidity and is particularly pathogenic, often associated with pulmonary infections and high mortality [1,3,4].…”