The Proteome Response of Salt-Sensitive Rapeseed (Brassica napus L.) Genotype to Salt Stress

Dolatabadi, Nima; Toorchi, Mahmoud; Valizadeh, Mostafa; Bandehagh, Ali

doi:10.15835/nbha47111133

Cited by 13 publications

(5 citation statements)

References 29 publications

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“…In addition, the increase or decrease of protein band intensity in response to salinity stress has also manifested. This result agrees with the findings of Dolatabadi and Toorchi (2017), who reported the presence or the absence of new protein bands in canola genotypes under salinity stress. The changes in protein profiles may be due to an inhibiting or stimulating translation of mRNAs under salinity stress or a result of the regulation of mRNA transcription (Hurkman and Tanaka, 1987).…”

Section: Discussionsupporting

confidence: 93%

Combining Ability for Yield, Oil Content, and Physio-Biochemical Characters of Canola (Brassica Napus L.) Under Salt Stress Conditions

ALSHARARI,

IBRAHIM,

OKASHA

2023

SABRAOJBG

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Creating a half-diallel cross succeeded among seven diverse canola genotypes. The obtained 21 F1 hybrids with their seven parents underwent three salinity stress levels exposure—3.91 dsm1 (Normal), 6.24 dsm-1 (S1), and 7.81 dsm-1 (S2) —during the 2020/2021 growing seasons. Salinity treatments significantly reduced days to 50% flowering, plant height, number of primary branches, pods/plant, 1000-seed weight, seed yield/plant, seed oil content, relative water content, calcium, potassium, and the ratio between K+ and Na+ compared with a normal condition. Proline content, osmotic pressure, and Na+ were considerably higher under salinity stress conditions. Highly significant differences showed among the parents and hybrids for all traits across the tested environments. General (GCA) and specific (SCA) combining ability effects were highly significant for all attributes. The parental genotypes Serw4 and Pactol resulted as good general combiners for increased seed oil content (SOC), seed yield/plant (SYPP), and some of its components in research environments. The hybrid combinations H2/S × Serw4 and Serw4 × Serw6 were good specific combiners for days to first flower (DTF), number of primary branches (NPB), number of pods per plant (NP), a thousand seed weight (TSW), seed yield per plant (SYPP), seed oil content (SOC), proline content (ProC), Ca++, and K+/Na+. The SDS-PAGE analysis of seed proteins indicated high levels of genetic variability and revealed some vital biochemical markers for salt tolerance.

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Section: Discussionsupporting

confidence: 93%

Combining Ability for Yield, Oil Content, and Physio-Biochemical Characters of Canola (Brassica Napus L.) Under Salt Stress Conditions

ALSHARARI,

IBRAHIM,

OKASHA

2023

SABRAOJBG

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“…This will help improve the understanding of the various molecular mechanisms operating under salinity in rapeseed and aid in developing GE rapeseed with increased salinity tolerance. 168 Recently, 2-DE was used for the separation of root proteins, while MALDI-TOF-MS was employed for protein identification under salinity. Different protein spots were detected and their abundance was significantly influenced by salinity.…”

Section: The Interplay Of Omics Approaches To Address Abiotic Stressesmentioning

confidence: 99%

Omics: The way forward to enhance abiotic stress tolerance inBrassica napusL

et al. 2021

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Plant abiotic stresses negative affects growth and development, causing a massive reduction in global agricultural production. Rapeseed (Brassica napus L.) is a major oilseed crop because of its economic value and oilseed production. However, its productivity has been reduced by many environmental adversities. Therefore, it is a prime need to grow rapeseed cultivars, which can withstand numerous abiotic stresses. To understand the various molecular and cellular mechanisms underlying the abiotic stress tolerance and improvement in rapeseed, omics approaches have been extensively employed in recent years. This review summarized the recent advancement in genomics, transcriptomics, proteomics, metabolomics, and their imploration in abiotic stress regulation in rapeseed. Some persisting bottlenecks have been highlighted, demanding proper attention to fully explore the omics tools. Further, the potential prospects of the CRISPR/Cas9 system for genome editing to assist molecular breeding in developing abiotic stress-tolerant rapeseed genotypes have also been explained. In short, the combination of integrated omics, genome editing, and speed breeding can alter rapeseed production worldwide.

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“…Previous studies had demonstrated that under salt stress, electrons with high energy would be transferred to molecular O 2 to cause excessive production of ROS and oxidative stress [ 9 ]. In our study, the activity of SOD and CAT at both seedling and flowering stages significantly increased as soil salinity increased.…”

Section: Discussionmentioning

confidence: 99%

“…Na + is the predominant cation in saline soil. The previous study reported that, as salinity increased from 0 to 300 mM NaCl L −1 , leaf Na + content increased, resulting in a decrease in shoot dry weight and plant height in rapeseed [ 9 ]. Cl − is a frequently occurring negatively charged ion in saline soil [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

Phenotype, Biomass, Carbon and Nitrogen Assimilation, and Antioxidant Response of Rapeseed under Salt Stress

Wang,

Lin,

et al. 2024

Plants

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Salt stress is one of the major adverse factors affecting plant growth and crop production. Rapeseed is an important oil crop, providing high-quality edible oil for human consumption. This experiment was conducted to investigate the effects of salt stress on the phenotypic traits and physiological processes of rapeseed. The soil salinity was manipulated by setting three different levels: 0 g NaCl kg−1 soil (referred to as S0), 1.5 g NaCl kg−1 soil (referred to as S1), and 3.0 g NaCl kg−1 soil (referred to as S2). In general, the results indicated that the plant height, leaf area, and root neck diameter decreased with an increase in soil salinity. In addition, the biomass of various organs at all growth stages decreased as soil salinity increased from S0 to S2. The increasing soil salinity improved the distribution of biomass in the root and leaf at the seedling and flowering stages, indicating that rapeseed plants subjected to salt stress during the vegetative stage are capable of adapting their growth pattern to sustain their capacity for nutrient and water uptake, as well as leaf photosynthesis. However, as the soil salinity increased, there was a decrease in the distribution of biomass in the pod and seed at the maturity stage, while an increase was observed in the root and stem, suggesting that salt stress inhibited carbohydrate transport into reproductive organs. Moreover, the C and N accumulation at the flowering and maturity stages exhibited a reduction in direct correlation with the increase in soil salinity. High soil salinity resulted in a reduction in the C/N, indicating that salt stress exerted a greater adverse effect on C assimilation compared to N assimilation, leading to an increase in seed protein content and a decrease in oil content. Furthermore, as soil salinity increased from S0 to S2, the activity of superoxide dismutase (SOD) and catalase (CAT) and the content of soluble protein and sugar increased by 58.39%, 33.38%, 15.57%, and 13.88% at the seedling stage, and 38.69%, 22.85%, 12.04%, and 8.26% at the flowering stage, respectively. In summary, this study revealed that salt stress inhibited C and N assimilation, leading to a suppressed phenotype and biomass accumulation. The imbalanced C and N assimilation under salt stress contributed to the alterations in the seed oil and protein content. Rapeseed had a certain degree of salt tolerance by improving antioxidants and osmolytes.

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The Proteome Response of Salt-Sensitive Rapeseed (Brassica napus L.) Genotype to Salt Stress

Cited by 13 publications

References 29 publications

Combining Ability for Yield, Oil Content, and Physio-Biochemical Characters of Canola (Brassica Napus L.) Under Salt Stress Conditions

Combining Ability for Yield, Oil Content, and Physio-Biochemical Characters of Canola (Brassica Napus L.) Under Salt Stress Conditions

Omics: The way forward to enhance abiotic stress tolerance inBrassica napusL

Phenotype, Biomass, Carbon and Nitrogen Assimilation, and Antioxidant Response of Rapeseed under Salt Stress

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