Omics: The way forward to enhance abiotic stress tolerance in<i>Brassica napus</i>L

Raza, Ali; Razzaq, Ali; Mehmood, Sundas Saher; Hussain, Muhammad; Su, Wei; He, Hairong; Zaman, Qamar U.; Zhang, Xuekun; Hasanuzzaman, Mirza

doi:10.1080/21645698.2020.1859898

Cited by 62 publications

(44 citation statements)

References 207 publications

(256 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Rapeseed ( Brassica napus L.) is the second imperative oilseed crop and possesses a complex genome. Several abiotic stresses significantly limit rapeseed productivity [ 33 , 34 , 35 , 36 ]. To date, SOD family genes have not been reported in rapeseed.…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Analysis and Expression Profile of Superoxide Dismutase (SOD) Gene Family in Rapeseed (Brassica napus L.) under Different Hormones and Abiotic Stress Conditions

Raza

Gao

et al. 2021

Antioxidants

Self Cite

View full text Add to dashboard Cite

Superoxide dismutase (SOD) is an important enzyme that acts as the first line of protection in the plant antioxidant defense system, involved in eliminating reactive oxygen species (ROS) under harsh environmental conditions. Nevertheless, the SOD gene family was yet to be reported in rapeseed (Brassica napus L.). Thus, a genome-wide investigation was carried out to identify the rapeseed SOD genes. The present study recognized 31 BnSOD genes in the rapeseed genome, including 14 BnCSDs, 11 BnFSDs, and six BnMSDs. Phylogenetic analysis revealed that SOD genes from rapeseed and other closely related plant species were clustered into three groups based on the binding domain with high bootstrap values. The systemic analysis exposed that BnSODs experienced segmental duplications. Gene structure and motif analysis specified that most of the BnSOD genes displayed a relatively well-maintained exon–intron and motif configuration within the same group. Moreover, we identified five hormones and four stress- and several light-responsive cis-elements in the promoters of BnSODs. Thirty putative bna-miRNAs from seven families were also predicted, targeting 13 BnSODs. Gene ontology annotation outcomes confirm the BnSODs role under different stress stimuli, cellular oxidant detoxification processes, metal ion binding activities, SOD activity, and different cellular components. Twelve BnSOD genes exhibited higher expression profiles in numerous developmental tissues, i.e., root, leaf, stem, and silique. The qRT-PCR based expression profiling showed that eight genes (BnCSD1, BnCSD3, BnCSD14, BnFSD4, BnFSD5, BnFSD6, BnMSD2, and BnMSD10) were significantly up-regulated under different hormones (ABA, GA, IAA, and KT) and abiotic stress (salinity, cold, waterlogging, and drought) treatments. The predicted 3D structures discovered comparable conserved BnSOD protein structures. In short, our findings deliver a foundation for additional functional investigations on the BnSOD genes in rapeseed breeding programs.

show abstract

Section: Introductionmentioning

confidence: 99%

Genome-Wide Analysis and Expression Profile of Superoxide Dismutase (SOD) Gene Family in Rapeseed (Brassica napus L.) under Different Hormones and Abiotic Stress Conditions

Raza

Gao

et al. 2021

Antioxidants

Self Cite

View full text Add to dashboard Cite

show abstract

“…Wind erosion of surface soils and leaching to groundwater is reduced when metal-tolerant plants are planted in such areas (Mthembu et al, 2020). (Raza et al, 2021b). Although an outstanding revolution has been accomplished in the biotechnological era, many queries and bottlenecks presently limit the utilization of omics approaches for stress tolerance research in several plant species.…”

Section: Phytostabilizationmentioning

confidence: 99%

Advances in “Omics” Approaches for Improving Toxic Metals/Metalloids Tolerance in Plants

et al. 2022

Self Cite

View full text Add to dashboard Cite

Food safety has emerged as a high-urgency matter for sustainable agricultural production. Toxic metal contamination of soil and water significantly affects agricultural productivity, which is further aggravated by extreme anthropogenic activities and modern agricultural practices, leaving food safety and human health at risk. In addition to reducing crop production, increased metals/metalloids toxicity also disturbs plants’ demand and supply equilibrium. Counterbalancing toxic metals/metalloids toxicity demands a better understanding of the complex mechanisms at physiological, biochemical, molecular, cellular, and plant level that may result in increased crop productivity. Consequently, plants have established different internal defense mechanisms to cope with the adverse effects of toxic metals/metalloids. Nevertheless, these internal defense mechanisms are not adequate to overwhelm the metals/metalloids toxicity. Plants produce several secondary messengers to trigger cell signaling, activating the numerous transcriptional responses correlated with plant defense. Therefore, the recent advances in omics approaches such as genomics, transcriptomics, proteomics, metabolomics, ionomics, miRNAomics, and phenomics have enabled the characterization of molecular regulators associated with toxic metal tolerance, which can be deployed for developing toxic metal tolerant plants. This review highlights various response strategies adopted by plants to tolerate toxic metals/metalloids toxicity, including physiological, biochemical, and molecular responses. A seven-(omics)-based design is summarized with scientific clues to reveal the stress-responsive genes, proteins, metabolites, miRNAs, trace elements, stress-inducible phenotypes, and metabolic pathways that could potentially help plants to cope up with metals/metalloids toxicity in the face of fluctuating environmental conditions. Finally, some bottlenecks and future directions have also been highlighted, which could enable sustainable agricultural production.

show abstract

“…Particularly, the PMI field has been given special attention due to the role metabolites play, not only as metabolic intermediates, but also as co-factors or ligands with the capacity to alter protein confirmations and functions ( Scossa et al, 2021 ). Detailed discussions on the advances made in plant proteomics can be accessed in numerous previous reviews ( Agrawal et al, 2013 ; Ghatak et al, 2017 ; Kosova et al, 2018 ; Labuschagne, 2018 ; Raza et al, 2021a ; Sinha and Verma, 2021 ).…”

Section: Omics Facilitated Crop Improvement For Abiotic and Biotic Stress Resistancesmentioning

confidence: 99%

Omics-Facilitated Crop Improvement for Climate Resilience and Superior Nutritive Value

et al. 2021

View full text Add to dashboard Cite

Novel crop improvement approaches, including those that facilitate for the exploitation of crop wild relatives and underutilized species harboring the much-needed natural allelic variation are indispensable if we are to develop climate-smart crops with enhanced abiotic and biotic stress tolerance, higher nutritive value, and superior traits of agronomic importance. Top among these approaches are the “omics” technologies, including genomics, transcriptomics, proteomics, metabolomics, phenomics, and their integration, whose deployment has been vital in revealing several key genes, proteins and metabolic pathways underlying numerous traits of agronomic importance, and aiding marker-assisted breeding in major crop species. Here, citing several relevant examples, we appraise our understanding on the recent developments in omics technologies and how they are driving our quest to breed climate resilient crops. Large-scale genome resequencing, pan-genomes and genome-wide association studies are aiding the identification and analysis of species-level genome variations, whilst RNA-sequencing driven transcriptomics has provided unprecedented opportunities for conducting crop abiotic and biotic stress response studies. Meanwhile, single cell transcriptomics is slowly becoming an indispensable tool for decoding cell-specific stress responses, although several technical and experimental design challenges still need to be resolved. Additionally, the refinement of the conventional techniques and advent of modern, high-resolution proteomics technologies necessitated a gradual shift from the general descriptive studies of plant protein abundances to large scale analysis of protein-metabolite interactions. Especially, metabolomics is currently receiving special attention, owing to the role metabolites play as metabolic intermediates and close links to the phenotypic expression. Further, high throughput phenomics applications are driving the targeting of new research domains such as root system architecture analysis, and exploration of plant root-associated microbes for improved crop health and climate resilience. Overall, coupling these multi-omics technologies to modern plant breeding and genetic engineering methods ensures an all-encompassing approach to developing nutritionally-rich and climate-smart crops whose productivity can sustainably and sufficiently meet the current and future food, nutrition and energy demands.

show abstract

Omics: The way forward to enhance abiotic stress tolerance inBrassica napusL

Cited by 62 publications

References 207 publications

Genome-Wide Analysis and Expression Profile of Superoxide Dismutase (SOD) Gene Family in Rapeseed (Brassica napus L.) under Different Hormones and Abiotic Stress Conditions

Genome-Wide Analysis and Expression Profile of Superoxide Dismutase (SOD) Gene Family in Rapeseed (Brassica napus L.) under Different Hormones and Abiotic Stress Conditions

Advances in “Omics” Approaches for Improving Toxic Metals/Metalloids Tolerance in Plants

Omics-Facilitated Crop Improvement for Climate Resilience and Superior Nutritive Value

Contact Info

Product

Resources

About