The protein antigens secreted in vivo by adult male Schistosoma mansoni

Abstract

Adult schistosomes can be transferred surgically from donor C57BL\6 mice to the portal veins of naive recipients with complete success. This procedure bypasses larval development and the antibody response of the host is directed against, and can be used to identify, those antigens released only by viable, mature parasites. Serum collected from the recipient mice (WTS) was used in Western blotting studies to probe fractionated parasite protein. Twelve immunodominant proteins were identified, ranging in molecular weight from 14 to 208 kDa. The magnitude of the IgG response against each antigen could be divided into 2 categories, on the basis of optical densitometry of the blots. In addition, defined parasite fractions were probed with WTS by Western blotting, in order to determine the relative abundance and distribution of each antigen in schistosome tissue. To confirm and expand on these initial observations, oligospecific polyclonal antibody for each immunogen was affinity purified from Western blots ; it was then used in immunocytochemistry to identify the sources of secretion for 8 of the 12 antigens, at the cellular level. From the results, it appeared that after the transfer of adult worms, the first antibodies detected were mostly directed against the gastrodermis. At later times additional reactivity was expressed against the tegumental membrane. These differences probably reflect the relative abundances of the gut and tegumental secretory products.

Immunoblot analysis ofSchistosoma spindaleexcretory–secretory antigens with sera from naturally infected bovines

“…The 28 kDa fraction could correspond to the 28 kDa GST reported Cutts and Wilson (1997). Less prominent bands in the immunoblot profile obtained in the present work included 14, 15, 16, 30, 34, 39, 47 and 57 kDa.…”

“…The ESAs of several other Schistosome spp. were prepared by different in vitro culture techniques in artificially supplemented media (Call et al 1995;Cutts & Wilson 1997;Perez-Sanchez et al 2006;El-Ridi & Tallima 2009). But we adopted an alternate strategy of incubating adult flukes in PBS (pH 7.4) for definite time-temperature combinations.…”

Concomitant Immunity to Schistosoma mansoni in Mice

“…This response was found against challenge infection in rhesus monkeys, hamsters rabbits and mice (5,7,8). Larval exposure appears to be central to the CI found in lymphatic filariasis (9).…”

Endothelial P2X7 receptors’ expression is reduced by schistosomiasis

“…Worms and eggs, as well as their soluble antigens, interact with host endothelium [4][5][6], causing its activation [7,8].…”