Immunoblot analysis of<i>Schistosoma spindale</i>excretory–secretory antigens with sera from naturally infected bovines

Lakshmanan, Bindu; Devada, K.; Joseph, Siju; Radhika, R.

doi:10.1080/09712119.2015.1031770

Cited by 5 publications

(3 citation statements)

References 20 publications

(23 reference statements)

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“…The present study involved preparation of ES antigen against the whole worm homogenates because ES antigens evoke a considerably higher degree of protective immunity in lab animals than the somatic antigen derived from whole worm (24). Lack of cross reactivity and the diagnostic specificity of ES antigen was confirmed and strong recommendation for the incorporation of ES antigen in various immunodiagnostic assays for detection of S. spindale was advised (17).…”

Section: Discussionmentioning

confidence: 99%

“…The worms were checked for viability under the microscope and mixed adult S. spindale worms were soaked in Phosphate Buffer Saline for two hours at room temperature followed by overnight incubation at 4°C. After incubation under sterile condition, the worms were carefully removed, the remaining filtrate was collected and centrifuged at 10,000 xg for 30 minutes at 4°C and the supernatant was used as ES antigen (16, 17). The protein concentration of ES antigen was estimated using protein estimation kit by Bradford method (Merck GeNei™, Bangalore).…”

Section: Methodsmentioning

confidence: 99%

“…After incubation under sterile condition, the worms were carefully removed, the remaining filtrate was collected and centrifuged at 10,000 xg for 30 minutes at 4 C and the supernatant was used as ES antigen (16,17). The protein concentration of ES antigen was estimated using protein estimation kit by Bradford method (Merck GeNei™, Bangalore).…”

Section: Es Antigen Preparationmentioning

confidence: 99%

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Development and Evaluation of a Colloidal Gold Immunofiltration Assay for Penside Diagnosis of Visceral Schistosomosis in Cattle

Mamane

Jeyathilakan

Latha

et al. 2022

Preprint

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BackgroundVisceral schistosomosis caused by Schistosoma spindale is an economically important chronic wasting snail-borne trematode infection which often goes undiagnosed in field condition due to lack of efficient diagnostic assays. ELISA and Colloidal gold immunofiltration assay were used to evaluate the state of active visceral schistosomosis in cattle through detection of antibodies against S. spindale ES antigen.Methodology/Principle FindingsThe present study included development and evaluation of enzyme linked immunosorbent assay (ELISA) and colloidal gold immunofiltration assay (CGIFA) with conventionally prepared S. spindale excretory-secretory (ES) antigen using postmortem mesenteric examination as a reference standard. Statistical analysis was performed using Cochran Q test, Chi-square test and kappa statistics which showed no significant difference between the developed tests and strong agreement with the postmortem examination which was considered as gold standard test. CGIFA showed sensitivity, specificity and accuracy of 92.98 %, 96.74 % and 95.55 % respectively whereas that of ELISA was 94.73 %, 95.12 % and 95% respectively.Conclusion/SignificanceCGIFA was found to be simpler, rapid, effective, less expensive and required least expertise for interpretation of results. It can be used as portable diagnostic device for rapid diagnosis of S. spindale infection at field condition making it an ideal pen side diagnostic kit.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Es Antigen Preparationmentioning

confidence: 99%

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