The protective effect of <i>Bifidobacterium bifidum</i> G9-1 against mucus degradation by <i>Akkermansia muciniphila</i> following small intestine injury caused by a proton pump inhibitor and aspirin

Yoshihara, Tsutomu; Oikawa, Yosuke; Kato, Takayuki; Kessoku, Takaomi; Kobayashi, Takashi; Kato, Shingo; Misawa, Noboru; Ashikari, Keiichi; Fuyuki, Akiko; Ohkubo, Hidenori; Higurashi, Takuma; Tateishi, Yoko; Tanaka, Yoshiki; Nakajima, Shunji; Ohno, Hiroshi; Wada, Koichiro; Nakajima, Atsushi

doi:10.1080/19490976.2020.1758290

Cited by 44 publications

(36 citation statements)

References 63 publications

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“…Moreover, we demonstrated that probiotics treatment (BBG9-1, Bifidobacterium bifidum) recovered the altered gut microbiome profile toward that in healthy controls and furthermore improved enhanced mucosal permeability [8]. This finding is well consistent with the fact that BBG9-1 has a protective effect on intestinal mucosal integrity by correcting gut flora in various dysbiotic animal models [9][10][11]. Interestingly, we also clarified that BBG9-1 treatment prevents the susceptibility to early life stress, suggesting that BBG9-1 may play a beneficial role not only in mucosal integrity but also in the function of the brain-gut axis.…”

Section: Introductionsupporting

confidence: 81%

Probiotic Bifidobacterium bifidum G9-1 Has a Preventive Effect on the Acceleration of Colonic Permeability and M1 Macrophage Population in Maternally Separated Rats

et al. 2021

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Although probiotics may be useful for the treatment of irritable bowel syndrome (IBS), it is unclear how probiotics play a role in colonic mucosal integrity and immunity. Here, we aimed to investigate the effect of Bifidobacterium bifidum G9-1 (BBG9-1) on colonic mucosal integrity and macrophage behavior in rats subjected to maternal separation (MS) as a model of IBS. MS pups were individually separated from their mother rats, and a proportion of the MS rats were orally administered BBG9-1. The colonic mucosal permeability was evaluated by Ussing chamber assay. The expression of tight junction proteins and cytokines and the population of CD80-positive cells was examined in the colonic tissues by real-time reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry. Caco2 cells were stimulated with cytokines and the transepithelial electric resistance (TEER) was measured. MS rats showed significantly higher colonic permeability and lower claudin 4 expression in the colonic epithelium relative to controls. The number of CD80-positive macrophages was significantly increased in the colonic mucosa of MS rats, accompanied by the increase of IL-6 and IFN-γ expression. BBG9-1 treatment ameliorated the increase of M1 macrophage and IL-6/IFN-γ expression in the colonic tissue of MS rats. Simultaneously, BBG9-1 treatment improved the enhanced mucosal permeability and the decreased claudin 4 expression in the colon of MS rats. IL-6 and IFN-γ, whose expression is enhanced in the colon of MS rats, significantly decreased TEER in Caco2 cells in vitro. Probiotic BBG9-1 has a preventive effect on the acceleration of colonic permeability and M1 macrophage population in maternally separated rats.

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Section: Introductionsupporting

confidence: 81%

Probiotic Bifidobacterium bifidum G9-1 Has a Preventive Effect on the Acceleration of Colonic Permeability and M1 Macrophage Population in Maternally Separated Rats

et al. 2021

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“…Interestingly, several studies have reported that resveratrol administration alters the composition and function of the gut microbiome of obese mice, and these have been characterized by a decreased abundance of Akkermansia [ 38 ]. Moreover, omeprazole-induced dysbiosis of the intestinal flora promotes the growth of Akkermansia and inhibits bifidobacterial growth, thus leading to thinning of the mucus layer through a reduced number of goblet cells in the small intestine [ 39 ]. Therefore, Akkermansia species may play diverse roles in the regulation of intestinal functioning, and exploring their relationship with other microorganisms in the gut environment might elucidate these roles.…”

Section: Discussionmentioning

confidence: 99%

Chlorogenic Acid Alleviates Colon Mucosal Damage Induced by a High‐Fat Diet via Gut Microflora Adjustment to Increase Short‐Chain Fatty Acid Accumulation in Rats

Xie

Fei

et al. 2021

Oxidative Medicine and Cellular Longevity

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A high-fat diet (HFD) has been previously associated with the development of diseases such as chronic colitis. While chlorogenic acid (CGA) is known to exhibit potent antioxidant, antibacterial, and anti-inflammatory properties, little is known about its effects on intestinal inflammation. In this study, we investigated the effects of CGA on intestinal inflammation in an HFD-induced obesity rat model and assessed whether these effects were related to changes in gut microbiota composition. This was achieved by examining physiological and biochemical indicators, the liver transcriptome, and the structure of the fecal microflora. CGA treatment significantly reduced HFD-induced internal organ weight gain, promoted colon tissue repair, downregulated the expression of inflammatory cytokines, and promoted the accumulation of the tight junction protein. KEGG enrichment analysis of differentially expressed genes, applied to data from the RNA-seq of rat liver tissue, revealed that CGA treatment significantly affected amino acid and lipid metabolism in the liver. Furthermore, CGA decreased the abundance of bacteria belonging to the genera Blautia, Sutterella, and Akkermansia and increased butyric acid levels, which were positively correlated with the abundance of Ruminococcus (butyric acid producer). Moreover, the beneficial changes observed in the HFD group were not as pronounced as those in the CGA treatment group. In summary, CGA can alleviate colitis in HFD-induced obesity through its anti-inflammatory effects associated with changes in gut microbiota composition and an increase in the production of short-chain fatty acids and thus can be used as a potential drug for the treatment of this pathology.

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“…Bifidobacterium pertains to the Actinobacteria phylum, which is usually considered a useful bacterial genus and has a pivotal role in the development, maturity and functional regulation of the immune system. 35 , 36 And the depletion of it has been reported in dozens of disease conditions 37 is seen as a characteristic of dysbiosis. Notably, a depletion of Bifidobacterium was discovered in the y-SHR-S compared with y-SHR ( p <0.01, n=4) ( Figure 10F ), this immensely diminished proportion of Actinobacteria and thus the decrease of gut microbiota diversity.…”

Section: Resultsmentioning

confidence: 99%

Restraint Stress in Hypertensive Rats Activates the Intestinal Macrophages and Reduces Intestinal Barrier Accompanied by Intestinal Flora Dysbiosis

Wang¹,

Gao²,

Yang³

et al. 2021

JIR

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Purpose: Hypertension (HTN) is a major risk factor for cardiovascular disease. In recent years, there were numerous studies on the function of stress in HTN. However, the gut dysbiosis linked to hypertension in animal models under stress is still incompletely understood. Purpose of this study is to use multiple determination method to determine the juvenile stage intestinal bacteria, cytokines and changes in hormone levels. Methods: Four groups of juvenile male spontaneously hypertensive rats (SHRs) and agematched male Wistar-Kyoto (WKY) rats were randomly selected as control and experimental groups. Rats in the two stress groups were exposed to restraint stress for 3 hours per day for 7 consecutive days. In one day three times in the method of non-invasive type tail-cuff monitoring blood pressure. The detailed mechanism was illuminated based on the intestinal change using immunohistochemical and immunofluorescence staining and the stress-related hormone and inflammation factors were analyzed via ELISA method. The integrity of the epithelial barrier was assessed using FITC/HRP and the expression levels of proteins associated with the tight junction was detected by Western blot. The alteration of stress-related intestinal flora from ileocecal junction and distal colon were also analyzed using its 16S rDNA sequencing. Results:The results indicate that acute stress rapidly increases mean arterial pressure which is positive correlation to hormone concentration, especially in SHR-stress group. Meanwhile, stress promoted the enhancement of epithelial permeability accompanied with a reduced expression of the tight junction-related protein and the macrophages (Mφ) aggregation to the lamina propria. There were remarkable significant increase of stress-related hormones and pro-inflammatory factor interleukin (IL)-6 along with a decrease in the diversity of intestinal flora and an imbalance in the F/B ratio. Conclusion:Our results reveal that stress accompanied with HTN could significantly disrupt the domino effect between intestinal flora and homeostasis.

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The protective effect of Bifidobacterium bifidum G9-1 against mucus degradation by Akkermansia muciniphila following small intestine injury caused by a proton pump inhibitor and aspirin

Abstract: Nakajima (2020) The protective effect of Bifidobacterium bifidum G9-1 against mucus degradation by Akkermansiamuciniphila following small intestine injury caused by a proton pump inhibitor and aspirin,

Cited by 44 publications

References 63 publications

Probiotic Bifidobacterium bifidum G9-1 Has a Preventive Effect on the Acceleration of Colonic Permeability and M1 Macrophage Population in Maternally Separated Rats

Probiotic Bifidobacterium bifidum G9-1 Has a Preventive Effect on the Acceleration of Colonic Permeability and M1 Macrophage Population in Maternally Separated Rats

Chlorogenic Acid Alleviates Colon Mucosal Damage Induced by a High‐Fat Diet via Gut Microflora Adjustment to Increase Short‐Chain Fatty Acid Accumulation in Rats

Restraint Stress in Hypertensive Rats Activates the Intestinal Macrophages and Reduces Intestinal Barrier Accompanied by Intestinal Flora Dysbiosis

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