The Protective Effect of Chrysin Against Cisplatin İnduced Ototoxicity in Rats

Abstract

Ototoxicity is a common side effect of cisplatin chemotherapy. The aim of this study was to investigate the potential protective effect of chrysin against cisplatininduced ototoxicity. Thirty-four adult female Wistar albino rats were separated into four groups: a cisplatin group (Group A), with cisplatin administered to ten rats once daily for three consecutive days at doses of 8 mg/kg body weight intraperitoneally (i.p.); a cisplatin plus chrysin group (Group B), with 8 mg/kg of cisplatin administered i.p. daily to ten rats for three consecutive days and 25 mg/kg of chrysin administered via oral gavage in a corn oil for 5 days: a chrysin group (Group C), with 25 mg/kg of chrysin administered via oral gavage in corn oil for 5 days to seven rats; and a control group (Group D), with 5 ml/kg of corn oil administered to seven rats via oral gavage for 5 days. Distortion product otoacoustic emission measurements were performed in the same ear of the rats under general anesthesia at baseline and on the first and fifth days after drug administration. No significant differences were noted between the measurements either in the chrysin group or in the control group. In the cisplatin group, there was a significant worsening of hearing compared to baseline and the measurements on the fifth day at all frequencies. In the statistical analysis, a statistically significant difference was observed at 5039, 6351, 8003, and 10078 Hz frequencies between the measurements on the first and fifth days. In the cisplatin plus chrysin group, there were statistically significant differences at frequencies of 2,003 and 5,039 Hz between the measurements at baseline and on the fifth day, at 3,175 and 5,039 Hz between the measurements on the first and fifth days, and at 8,003 and 100,078 Hz between the measurements at baseline and on the first day. According to these results, this study demonstrates that cisplatin-related ototoxicity can be prevented in rats by the administration of chrysin.

Investigation of Astaxanthin Effect on Cisplatin Ototoxicity in Rats by Using Otoacoustic Emission, Total Antioxidant Capacity, and Histopathological Methods

“…Some of these antioxidants are N-acetyl cysteine (NAC), sodium thiosulfate (STS), amifostine, lipoic acid, resveratrol, intratympanic administration of dexamethasone, intratympanic administration of Ringer lactate, erdosteine, a-tocopherol, chrysin, and quercetin. 1,[12][13][14][15] Especially, antioxidants such as NAC, a-tocopherol, lipoic acid, STS, salicylate, ebselen, D-methionine, and amifostine have been demonstrated to show a protective effect against cisplatin-induced ototoxicity in animal studies and in vitro studies. Clinical studies performed in humans regarding cisplatin-induced ototoxicity are very limited.…”

Possible protection against cisplatin induced behavioral changes by dietary antioxidants in adult albino mice.

“…These results are in line with other studies conducted on mice and rats, which show ototoxicity in platinium treated animals, Kelles and colleges showed ototoxicity when cisplatin was administered in mice. 19…”

The effect of genistein on cisplatin induced ototoxicity and oxidative stress

“…The most significant mechanisms are an increase in the generation of reactive oxygen species (ROS) and a reduction in the antioxidant enzyme system in the cochlea. 7 Disruption of this balance within the cell is likely to result in cochlear cell damage and death. This balance is aimed to be restored by using antioxidant agents.…”