Cardiovascular disease and its complications are mainly causes of coronary artery disease (CAD). The distribution width of red blood cells (RDW) is a quantitative measurement of the variance in circulating erythrocyte size. Various research publications have shown that patients with previous history coronary artery disease having greater levels of Red cell distribution width are on risk of mortality and cardiovascular events. We tested the hypothesis that Red cell distribution width, Hct, and other red blood corpuscle (RBC) indices are associated with CAD. Hence, we measured RDW, Hct, and other RBC indices in AMI and stable CAD (SCAD) and compared them with age- and sex-matched controls. Objectives: To study the changes in Red cell distribution width and RBC indices in acute myocardial infarction (AMI) and SCAD and compare them with age- and sex-matched controls. Study Design: A comparative study. Setting: Department of Cardiology, Liaquat University Hospital. Period: 1st September 2013 to 28th February 2014. Material & Methods: 128 subjects (39 AMI patients, 24 SCAD patients and 65 controls). Venous samples from AMI subjects were collected in standardized ethylenediaminetetraacetic acid (EDTA) sample tubes on admission (within 6 h of chest pain). Using Sysmex KX21-N autoanalyzer, RDW and RBC indices were evaluated within 30 minutes of blood collection. Arterial blood samples were also obtained from stable CAD patients admitted to angiography and routine inspections. There has been no significant difference. Results: In total 128 patients, Mean ± SD of RDW patients with CAD was 14.12 ± 1.31%) as compared to controls (15.62 ± 6.51%) with insignificant difference (p value > 0.05). Mean ± SD of RDW patients with AMI was 14.36 ± 1.4% as compared to stable CAD (13.7 ± 1.09%) and controls (15.62 ± 6.51%) (p value > 0.05). Mean ± SD of RDW patients with Hct in patients with CAD was 43.16 ± 5% as compared to controls (41.9 ± 6.9%) with insignificant difference (p value > 0.05). Conclusions: There was no association between RWD, Hct, and other RBC indices with CAD, AMI, and stable CAD.
Objectives: To analyze the antioxidant activity of Resveratrol and Ascorbic Acid against hydrogen peroxide (an oxidant) mediated cell injury of human trabecular meshwork cells. Study Design: Experimental study. Setting: Molecular Biology Laboratory at Medical Research Centre, Liaquat University of Medical & Health Sciences, Jamshoro. Period: Six months. Materials and Methods: Human Trabecular Meshwork cells were purchased from ScienCell Research Laboratories, USA. TM cell metabolism, TM cell viability and Reactive oxygen species were detected by standard methods in co- and pre- treated TM cells. Results: A significant reduction in TM cell metabolism was observed approximating 61% at 1.0 mM H2O2 compared to Ascorbate – 99% and Resveratrol 99% (p=0.0001). Resveratrol was more effective than Ascorbate even at 4.0 mM H2O2, the TM cell activity was noted 76%. Compared to H2O2- treated TM cells, resveratrol improved mitochondrial function upto 4.0 mM H2O2 (76%). Compared to co-treatment, the pretreatment shows similar results except at 4.0 mM H2O2. At 4.0 mM H2O2 the pre-treat TM cell metabolic activity was found as 11%, 31% and 47% compared to co-treat as 9%, 31% and 76% in controls, ascorbate and resveratrol groups respectively (p<0.05). Resveratrol shows significant decrease in viability was seen in controls compared to Ascorbate and Resveratrol groups. Cell viability showed statistically significant differences at 2.0 and 4.0 mM H2O2 compared to controls (P=0.0001). For reactive oxygen species (ROS), cells were incubated and with Ascorbate and Resveratrol for 24 hours and TM cells were treated with 0.0mM, 0.5 mM, 1.0 mM, 2.0mM and 4.0mM H2O2. Significant decrease in ROS was noted by Resveratrol compared to Ascorbate. Conclusions: Resveratrol and Ascorbate may prove useful in preventing and delaying the glaucoma, and timely institution of these anti – oxidants may help maintain trabecular meshwork functions and prevent visual loss.
Background: Bisphenol A (BPA), an endocrine disruptor, may be involved in the etiology of autism spectrum disorders (ASD); however, the mechanism of neuronal and astrocytic damage remains ambiguous. A possible role of altered expression of p21 in autistic-like behavior in rat offspring was examined with prenatal and postnatal BPA exposure.Methods: Wistar albino dams were exposed to BPA (5 mg/kg) intraperitoneally throughout pregnancy and until the third postnatal day (PND). Pups were examined on 21st PND for behavioral test. Blood samples were collected for serum lactate levels and pups were sacrificed. Right frontal cortices were dissected out and processed for H&E, immunohistochemical analysis, and gene expression. Results: Anxiety like behavior and thigmotaxis along with reduction in serum lactate concentrations were observed in pups exposed to BPA. Decline in neuronal number and decreased astrocytic population with reduced dendritic spines were revealed by H&E and immunohistochemical analysis, respectively, in right frontal cortices. Over expression of p21 was also detected in BPAexposed offspring.Conclusions: Over expression of p21 may be associated with autistic behavior. Further studies are recommended to explore the structural alterations in other white matter pathways in frontal cortices.
Background: Senile cataract is a common cause of blindness among older people. The problem is common among adults of age 40 and older. There are various factors that cause senile cataracts among adults. These factors include protein and lipid variations, genetic role, and the existence of trace elements like magnesium, zinc and copper. Aim and objectives: To evaluate the level of zinc and relationship between copper, and magnesium in patients with senile cataract by comparing with healthy subjects. Material and Methods: This study has a key focus on examining the effect of trace elements on senile cataract patients. We conducted experiments when 100 people (50 patients and 50 non-patients). Absorption spectrometry was used to examine the blood samples of the people who took part in the research. Results: It was noted during the results that most of the patients developed senile cataracts after crossing the age of 50. It was also noted from the results that the concentration of copper and zinc was higher in the patients as compared to the non-patients, while the concentration of magnesium was lower in the patients as compared to the non-patients. Based on these results, it can be stated that an increase in the concentration of copper and zinc increases the chances of senile cataracts. The decline in the concentration of magnesium increases the chances of senile cataracts. Also, out of these patients, a majority of them were females. Therefore, there are chances of biased results in this study that can be improved in future studies with the number of patients and an equal number of males and females. Conclusion: The concentrations of copper and zinc were significantly higher in patients than in the non-patients, whereas the concentration of magnesium was lower in the patients than in the non-patients. Based on these findings, it may be concluded that an increase in copper and zinc concentrations increases the risk of senile cataracts. Keywords: zinc, copper, magnesium, senile cataract, healthy subjects.
Objectives: To evaluate the effects of aspiration thrombectomy (AT) using anAspiration Catheter (INVATEC SPA DIVER C.E. MAX, Italy) in patients with acute myocardialinfarction (AMI). Study Design: Cross sectional and Case series study. Setting: Departmentof Cardiology, Liaquat University Hospital, Hyderabad. Period: January 2012 to December2013. Methodology and Results: Measurement of left ventricular ejection fraction (LVEF) byechocardiography was obtained before (acute LVEF) percutaneous coronary intervention (PCI)and 1 week after (late LVEF) the procedure. Most of the patients with full restoration of STsegment elevation following PCI were higher in the aspiration Catheter control group (90 vs68%, P < 0.01), (86 vs 62%, P < 0.01) and (90 vs 64%, P < 0.01) respectively. Minimal changein LVEF was observed in patients between the Aspiration Catheter group and control group at1 week post procedure. Conclusion: Combination of AT using Aspiration Catheter (INVATECSPA DIVER C.E. MAX) with primary stenting can better outcome, while left ventricle (LV) functionhad subtle change in AMI when compared to primary stenting after balloon predilation withoutthrombectomy.
Objectives: To assess the outcome of vitamin D as adjunctive therapy in reducing the serum level of glycosylated hemoglobin (HbA1C) in type 2 diabetic patients taking anti-diabetic drug metformin. Study Design: An Observational study. Setting: This study was conducted at the Department of Pharmacology, Liaquat University of Medical and Health Sciences Jamshoro in collaboration with Sindh Institute of Endocrinology & Diabetes (SIED) Liaquat University of Medical and Health Sciences Jamshoro. Period: December 2017 to May 2018. Material and Methods: We carried out this study on 140 patients. Diagnosed patients of type 2 DM With duration more than 5 years, age between 35 to 60 years, HbA1c equal to or more than 6.0%, and diabetic patients which were on metformin and had vitamin D deficiency (level less than 30ng/ml) were included in the study. Results: Total 140 type 2 diabetic patients were taken in study. At baseline, in Group B, mean HbA1c ± SD was 7.92±1.54 while at 3 months intervention, in Group B, mean HbA1c ± SD was 7.18±1.53. No significant difference in HbA1c between Group A and B at baseline and after three months of intervention (P value = 0.46). The mean value of vitamin D before supplementation was 16.23±3.45 vs 28.96±5.25 after 3 months supplementation. There was a significant increase in 25 (OH) D levels after vitamin D supplementation after 3 months. (P < 0.0001). Conclusion: Supplementation of vitamin D did not show any effect on blood sugar control in our patients with type 2 DM.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.