The Protective Action of Dysentery Bacteriophage in Experimental Infections in Mice

Abstract

A review of the literature on the in vivo action of dysentery-phage does not enable one to formulate any definite conclusions as to its value (Morton and Engley, 1945). There are about twice as many favorable as unfavorable reports. However, the use of dysentery-phage in man has been made under such poorly planned or executed experiments as to invalidate the results. The logical sequence of events should have been the testing of dysentery-phage in experimental animals, after the discovery of its action in vitro, before trying it on humans. To our knowledge this was not done. We wished to know whether dysentery-phage had any action in vivo and whether it would be reasonable to expect any prophylactic or therapeutic action in man. We feel that the results of animal experiments to be reported answer both questions in the affirmative.

Amino Acid Requirements for the Maturation of Bacteriophage in Lysogenic Escherichia Coli

“…Media. The complex medium was a tryptoneyeast extract-glucose broth described by Morton and Engley (1945). In addition to this, nutrient broth and tryptone broth also were employed.…”

The Occurrence of Mitosis in Bacillus Megaterium and Its Relation to the Growth Cycle

“…Inocula were obtained from 24 hour cultures grown on agar slants (Morton and Engley, 1945) that had been seeded from a single clone. The slants were washed with 1 ml of physiological saline containing 0.1 per cent phosphate adjusted to pH 7.0.…”

Comparative Cytology of Wild Type Saccharomyces and a Respirationally Deficient Mutant

“…Cultures were grown in tryptone-yeast extractglucose broth (Morton and Engley, 1945) with or without added glucose. Observations were made on young actively growing (2 to 6 hour) and 18 hour cultures with a Bausch and Lomb phase contrast or, more often, by direct critical illumination with a Bausch and Lomb research light microscope.…”