Comparative Cytology of Wild Type Saccharomyces and a Respirationally Deficient Mutant

Abstract

Certain aspects of the cytology of wild type Saccharomyces cereviuiae have been compared with those of a stable respirationally deficient mutant derived from it. Paretsky and Clark (1953) have described some comparative biochemical characteristics of these two strains. MATERIALS AND METHODS I This work has been aided by a grant from the United States Atomic Energy Commission, A.E.C. Contract No. AT(30-1)-1342. ' Janus green B, Coleman and Bell Company, Norwood, Ohio, Cert. No. CJ-5. ' Neotetrazolium chloride, Montclair Research Corporation,

Studies on the Cytological Structure of Yeast: Electron Microscopy of Thin Sections

“…Mitochondria. Cytoplasmic particles possessing the specific staining properties of mitochondria have been described in Saccharomyces by several investigators (Lindegren, 1949;Hartman and Liu, 1954;Bautz, 1955a, b;and Yotsuyanagi, 1955). However, their small size and difficulties inherent in cytochemical methods have made their identification the subject of considerable controversy (Williams et al, 1956).…”

Fine Structure of Microorganisms

“…The structures (G in figure 1) which occur within the ascus wall, although not enclosed within the ascospore, stain deeply with Sudan black B, each being suirrounded bv a thick limiting membrane. These granules in common with vegetative cell mitochondria, are lipoidal in nature (Hartman and Liu, 1954;Sarachek and Townsend, 1953;Lindegren, 1949). Therefore it seems logical to conclude that these structures are only remnants of the mother cell mitochondria since cristae mitochondriales appear to be absent.…”

Metachromatic Granules of Microorganisms

“…Metaphosphate (volutin) is transiently associated chiefly with cytoplasmic granules and to a lesser extent with the central vacuole. In young yeast cells, lipid-soluble dyes and metaphosphate accumulated at the same site (Hartman and Liu, 1954).…”