The Prolactin-Inducible Protein / Gross Cystic Disease Fluid Protein (PIP/GCDFP-15): Genetic Analysis and Hormonal Regulation of Gene Expression

Shiu, Robert P. C.; Myal, Yvonne; Tsuyuki, Deborah; Robinson, David; Yarmill, A.; Watson, Peter H.

doi:10.1007/978-1-4615-3494-5_7

Cited by 3 publications

(10 citation statements)

References 8 publications

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“…It is identical to the gene of the prolactin-inducible protein (PIP), independently described by Shiu and co-workers (23,32) in the T47D cell line. Murphy et al (22) reported on the expression of this gene in primary human breast carcinomas.…”

Section: Introductionmentioning

confidence: 95%

PIP/GCDFP-15 gene expression and apocrine differentiation in carcinomas of the breast

Pagani

Sapino

Bergnolo

et al. 1994

Vichows Archiv A Pathol Anat

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The frequency and the significance of apocrine differentiation in carcinomas of the breast are uncertain, because of the lack of reliable and reproducible criteria for morphological diagnosis. The 15 kDa glycoprotein of cystic breast disease (GCDFP-15) is regarded as a specific functional marker of apocrine cells. Expression of the prolactin-inducible protein (PIP)/GCDFP-15 gene was investigated by Northern blot analysis and in situ hybridization in breast cancer cell lines and in an unselected series (33 cases) of primary carcinomas of the breast. On the same cases, histological assessment of apocrine differentiation and immunocytochemical detection of GCDFP-15 were also performed and correlated with follow-up data. The presence of PIP/GCDFP-15 mRNA was a feature of a relatively high number of cases, but was incompletely correlated with histological and immunocytochemical evidences of apocrine differentiation. Expression of the PIP/GCDFP-15 gene was significantly associated with relapse-free survival, and may represent a novel variable of functional and prognostic relevance.

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Section: Introductionmentioning

confidence: 95%

PIP/GCDFP-15 gene expression and apocrine differentiation in carcinomas of the breast

Pagani

Sapino

Bergnolo

et al. 1994

Vichows Archiv A Pathol Anat

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“…Much less is known about the expression of pS2 in metastatic breast carcinoma [8][9][10]12], The expression of pS2 in uninvolved breast tissue has been discussed in detail in an earlier communication [4], pS2-mRNA was found more frequently in uninvolved breast tissue of pre menopausal women than in that of postmenopausal wom en [4], While previously no differences in pS2 expression between breast carcinomas from pre-or postmenopausal women could be seen [ 1], in the present series pS2-mRNA was found more often in tumors from premenopausal women: 16 of 23 (70%) of tumors from women aged 50 or 86 Hahnel/Harvey/Robbins/Stcrrctt/Hahnel Hormone-Regulated Genes in Breast Tissue less expressed pS2, while only 30 or 65 (46%) of the older age group contained pS2-mRNA (p = 0.04). PlP-mRNA has been found in primary breast carcino ma with a frequency varying between about 25 [1] and over 60% [6]. In our current series approximately one third of primary breast carcinomas contained PIPmRNA.…”

Section: Discussionmentioning

confidence: 62%

“…PIP-mRNA is found in a proportion of breast carcinomas, and postmenopaus al patients are twice as likely to have high levels of PIPmRNA in a tumor as are premenopausal patients [ 1 ]. PIPmRNA has not been found previously in normal breast tissues [6].…”

Section: Introductionmentioning

confidence: 74%

“…Our data do not confirm this association: a similar proportion of PIP-positive and PIP-negative primary car cinomas was associated with lymph node métastasés ( 11 of 31 or 35% and 27 of 33 or 45%, respectively). Surpris ingly, previous reports [6,13] failed to demonstrate the expression of the PIP gene in normal breast tissue. We found strong signals for PIP-mRNA in about half of 71 uninvolved breast tissues from mastectomies, distinctly more than in breast carcinomas.…”

Section: Discussionmentioning

confidence: 96%

“…PIP-mRNA levels in cultured human breast cancer cells are stimulated by prolactin, androgen and glucocorti coid, and inhibited by estrogen [6]. PIP-mRNA is found in a proportion of breast carcinomas, and postmenopaus al patients are twice as likely to have high levels of PIPmRNA in a tumor as are premenopausal patients [ 1 ].…”

Section: Introductionmentioning

confidence: 99%

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Hormone-Regulated Genes (pS2, PIP, FAS) in Breast Cancer and Nontumoral Mammary Tissue

Hähnel¹,

Harvey²,

Robbins³

et al. 1994

Pathobiology

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Expression of the hormone-regulated genes, pS2, prolactin-inducible protein (PIP) and fatty acid synthetase (FAS), was investigated by Northern blotting in primary breast carcinoma, metastatic breast cancer in axillary lymph nodes, in uninvolved breast tissue from mastectomies and in normal lymph nodes. There were considerable differences in expression of the genes between the tissues. The proportion of tissues containing PIP-mRNA decreased from uninvolved breast tissue to primary breast carcinoma to metastatic carcinoma. The reverse applied to FAS-mRNA which was found more often in metastatic cancer than in primary cancer, and least frequently in uninvolved breast tissue. Yet another pattern was observed for pS2 expression. The highest proportion of tissues demonstrating gene expression was found in primary breast cancer with both metastatic tumor and uninvolved breast tissue expressing the gene less frequently. pS2-mRNA and PIP-mRNA could only rarely be detected in trace amounts in normal lymph nodes. In contrast, FAS-mRNA was present in about one third of normal lymph nodes. Only pS2-mRNA showed an association with estrogen and progesterone receptor status.

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Mitogenic effect of the 15‐kDa gross cystic disease fluid protein (GCDFP‐15) on breast‐cancer cell lines and on immortal mammary cells

Cassoni

Sapino

Haagensen

et al. 1995

Intl Journal of Cancer

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The biological significance of a major protein component in the fluid of gross cystic breast disease and a recognized marker component protein in breast gross cystic disease fluid and is Dresent at a concentration of aDDroximatelv 30 mg/ml. I I of apocrine-metaplasia, i.e. the I5-kDa glycoprotein (GCDFP-15). is presently unknown. We have added GCDFP-I5 to cell culture medium and tested its effect on proliferation of human breast-cancer cell lines (MCF7, BT474, MDA-MB23 I and ~4 7~) and a "normal" human immortal bmast-cell line (MCF 1 0~) .

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The Prolactin-Inducible Protein / Gross Cystic Disease Fluid Protein (PIP/GCDFP-15): Genetic Analysis and Hormonal Regulation of Gene Expression

Cited by 3 publications

References 8 publications

PIP/GCDFP-15 gene expression and apocrine differentiation in carcinomas of the breast

PIP/GCDFP-15 gene expression and apocrine differentiation in carcinomas of the breast

Hormone-Regulated Genes (pS2, PIP, FAS) in Breast Cancer and Nontumoral Mammary Tissue

Mitogenic effect of the 15‐kDa gross cystic disease fluid protein (GCDFP‐15) on breast‐cancer cell lines and on immortal mammary cells

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