“…A ten-fold serial dilution of each sample was prepared up to the fifth dilution. Cultures were made from each dilution on a selective media for S. mutans (Mitis salivarius bacitracin agar, MSBA, Sigma-Aldrich Chemie GmbH, Eschenstrasse 5, D-82024 TAUFKIRCHEN) and incubated anaerobically for 48 h at 37°, followed by 24 h in aerobic condition [ 21 ]. The streptococcus colonies were identified using Gram's stain, which appeared under a light microscope as; round or spherical, dark blue, pin point size, and arranged in chains.…”