The Prevalence of Shiga Toxin-Producing Escherichia coli and Enteropathogenic Escherichia coli Isolated from Raw Chicken Meat Samples

Abstract

Background. Shiga toxin-producing Escherichia coli (STEC) is known as a crucial zoonotic food-borne pathogen. A total of 257 raw chicken meat samples were collected from different markets in Hamadan, west of Iran, from January 2016 to May 2017. Materials and Methods. The samples were cultured in selective and differential culture media, and the virulence genes of E. coli isolates were analyzed by PCR assay. The antibiotic resistance patterns of E. coli isolates were determined by the disk diffusion method. The genetic relatedness of the E. coli O157 isolates was analyzed by ERIC-PCR. Results. In total, 93 (36% ± 3.12) of the isolates were identified as E. coli in this study. Based on serological and microbiological tests, 36 (38.7% ± 9.9), 7 (7.5% ± 5.35), and 12 (12.9% ± 6.81) of the E. coli isolates were characterized as STEC, enteropathogenic E. coli (EPEC), and attaching and effacing E. coli (AEEC) strains, respectively. A high level of resistance to nalidixic acid (91.4% ± 5.7), tetracycline (89.2% ± 6.31), ampicillin (82.8% ± 7.67), and trimotoprime-sulfametoxazole (71% ± 9.22) was detected among the E. coli isolates. The analysis of the ERIC-PCR results showed five different ERIC types among the E. coli O157 isolates. Conclusions. Based on our findings, control and check-up of poultry meats should be considered as a crucial issue for public health.

Prevalence of Shiga Toxin Producing Enterohemorrhagic Escherichia coli O157:H7 Isolated from Chicken Meat in Northern Punjab, Pakistan

“…In the current study, although the prevalence of this E. coli isolate is not remarkable, this infection rate is significant from public health perspective. The prevalence of this STEC isolate in present study agreed with the studies in Iran, Korea and some other countries (Zarei et al, 2021;Lee et al, 2009).…”

Survey of clinical and commensal Escherichia coli from commercial broilers and turkeys, with emphasis on high-risk clones using APECTyper

“…No toxin-encoding genes were found in the isolates linked to Enterohemorrhagic E. coli or Enterotoxigenic E. coli . Atypical EPEC ( aEPEC ) have been reported at relatively low frequency in broiler production and meat samples across the world but, to our knowledge, have not been reported in US broiler or turkey production ( Farooq et al, 2009 ; Tanabe, 2013 ; Alonso et al, 2016 ; Adorjan et al, 2020 ; Zarei et al, 2021 ). These results illustrate that aEPEC do inhabit the gastrointestinal tracts of poultry in the United States, and could represent a possible threat to human health.…”

Knowledge, Hygienic Practices, and Toxi-Infectious Risks Associated with Ready-to-Eat Gbeli: A Particular Chip Derived from Cassava (Manihot esculenta Crantz) Tuber Vended in Streets of Abomey-Calavi Municipality, Benin

“…This E. coli serotype is considered one of the most dangerous. Several epidemic of foodborne bacterial illnesses due to consumption of raw or undercooked meat contaminated with strains of STEC have been reported [ 37 ].…”