The prevalence of human papillomavirus type 16 and 18 DNA in cervical cancer in different age groups: A study on the incidental cases of cervical cancer in Norway in 1983

Sebbelov, Anne M.; Kjørstad, Kjell E.; Abeler, Vera M.; Norrild, Bodil

doi:10.1016/0090-8258(91)90274-9

Cited by 14 publications

(13 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The age of patients with cervical carcinomas had a two-peaked distribution curve, corresponding well with the findings in another Scandinavian study (14) Our HPV typing results are in agreement with other results based on PCR, with a preponderance of HPV 16 in squamous carcinomas (15)(16)(17)(18) The prevalence of HPV (86.6%) in this series was higher than in another recent Scandinavian study (is), which also utiliT.ed PCR for detection and typing of HPV. The higher detection rate in the present study was accomplished by the combined use of consensus primers directed against L1 and E1 and type-specific primers directed against E6 and E7.…”

Section: Discussionsupporting

confidence: 91%

Implications of human papillomavirus type for survival in cervical squamous cell carcinoma

Hagmar¹,

Christensen²,

Johansson

et al. 1995

Int J Gynecol Cancer

View full text Add to dashboard Cite

In a Swedish series of 107 invasive squamous carcinomas of the cervix, DNA extraction from paraffin-embedded material was successful in 97 cases. The prevalence of human papillomavirus (HPV) in this material was 86.6%, as determined by polymerase chain reaction (PCR) using both consensus and type-specific primers. HPV type 16 was most common (42.3%; other types were 31 (12.3%), 18 (9.3%) and 33 (10.3%). Seventeen cases (17.3%) were positive for the consensus primers only and were regarded as HPV of unknown type. There was no significant difference in corrected survival between patients with HPV-positive or -negative tumors. In the HPV-positive group, patients with tumors containing HPV 33 or HPV 18 had a significantly poorer prognosis than patients with tumors containing other types of HPV DNA (relative hazard 3.18, 95% confidence interval 1.37-7.39, P = 0.007), implying a prognostic significance of HPV type.

show abstract

Section: Discussionsupporting

confidence: 91%

Implications of human papillomavirus type for survival in cervical squamous cell carcinoma

Hagmar¹,

Christensen²,

Johansson

et al. 1995

Int J Gynecol Cancer

View full text Add to dashboard Cite

show abstract

“…We cannot exclude either that our results are biased by the retrospective nature of the study and that our observations could be attributable to some confounders that we have not allowed for. The age-specific mortality rate of the cervix carcinoma for younger women cannot be attributed to a new papilloma virus epidemic during the last 20 years, since Sebbelov et al have shown that the proportion of HPV positive patients is equal in all age groups [35]. Moreover, this age-specific mortality rate cannot be accounted for by difference in intrinsic radiosensitivity [361.…”

Section: Discussionmentioning

confidence: 99%

Younger age as a bad prognostic factor in patients with carcinoma of the cervix

Jf¹,

Pampallona²,

Coucke³

et al. 1996

European Journal of Obstetrics & Gynecology and Reproductiv

View full text Add to dashboard Cite

Objective: To verify the influence of age on the prognosis of cervix carcinoma. Study design: Five hundred and sixty eight patients treated for a FIGO stage IB-IVA with radical irradiation in the Centre Hospitalier Universitaire Vaudois of Lausanne were subdivided according to the following age categories: _<45, 46-60, 61-69 and > 70 years. Taking the 46-60 years age group as the reference, the hazard ratios (HR) of death and corresponding 95% confidence intervals (95% CI) were estimated by means of a Cox multivariate analysis. Results: The 5-year survival rates were, respectively, 57%, 67%, 60% and 45%. For the youngest women the risk of death was significantly increased (HR = 2.00, 95% CI [1.32-3.00]) and was even more accentuated in advanced stages. Conclusion: Age under 45 years is a bad prognostic factor in carcinoma of the cervix.

show abstract

“…One half was fixed in 10% phosphate-buffered formalin, embedded in paraffin, and stained with haematoxylin-eosin (HE) for histopathological diagnosis. The other half was snap frozen in liquid nitrogen and stored at -80°C for Southern blot hybridization (Sebbelov et al 26). In situ hybridization was carried out only on specimens where the Southern blot analysis had been performed on a minimum of 5 pg DNA.…”

Section: Methodsmentioning

confidence: 99%

“…Southern blot analysis on the specimens was done as earlier described (26). Briefly, the DNA was extracted from the biopsies according to standard procedures and quantitated by means of fluorescence spectrophotometry.…”

Section: Southern Blot Hybridization Proceduresmentioning

confidence: 99%

The prevalence of human papillomavirus in cervical lesions with koilocytosis and/or CINI

Iversen¹,

Duun

Sebbelov³

et al. 1992

APMIS

Self Cite

View full text Add to dashboard Cite

The prevalence of human papillomavirus in cervical lesions with koilocytosis and/or CIN I. APMIS 100: 280-286, 1992. Thirthy-one patients with koilocytosis and/or concomitant CIN I were analysed for the presence of HPV types 11, 16 and 18 by in situ hybridization and Southern blot analysis. The prevalence of HPV was 48%) and 55%, respectively, when measured by the two methods and among the HPV positive, HPV 11 and 16 were present in 47% and 60%, respectively, whereas HPV 18 was not found.Clinical epidemiological studies support the hypothesis that precancerous and cancerous lesions of the uterine cervix are related to human papillomavirus (HPV) infection (1,9,16,17, 33, 34). Distinct subtypes of HPV have been demonstrated in 60-100% of precancerous and malignant lesions of the cervix, and an essential event in the malignant transformation may be the integration of the viral genome into the chromosomal DNA (7,24,27, 32). More than 60 HPV types and subtypes have been cloned and characterized (6). At present more than 20 different HPV types have been associated with infections in the genital mucosa. HPV 6 and 11 are most frequently found in genital condylomas and in lower grade cervical intraepithelial neoplasia (CIN I) (reviewed by Bosch & Munoz (1). The HPV types 16, 18, 31, 33, 35, 45, 51, and 52 are most often associated with higher grades of dysplasia (CIN I1 and CIN III/CIS) and invasive carcinoma of the uterine cervix (1). The prevailing types are HPV 16 and 18, which can be demonstrated in 60-100% of specimens with CIN 11, 111 and invasive cancer (1, 32). Typing of HPV in early lesions is believed to be of prognostic value and allows identification of possible risk groups. It is therefore desirable to develop diagnostic methods which are reliable, fast and inexpensive. Genital lesions are detected by clinical examination and by analysis of histological and cytological specimens, but identification of HPV types is only possible by nucleic acid hybridization or polymerase chain reaction (PCR) (14,18, 32).Brigati, D. J., Myerson, D. & Leary, J. J.: Detection of viral genomes in cultured cells and paraffin-embedded tissue sections using biotin-labeled hybridization probes. J. Virol. 126: [32][33][34][35][36][37][38][39][40][41][42][43][44][45][46][47][48][49][50] 1983. Campion, M. J., McCance, D. J., Cuzick, J. & Singer, A.; Progressive potential of mild cervical atypia: prospective cytological, colposcopic, and virological study. Lancet Aug 2: 237-240, 1986. Caussy , D., Marrett, L. D., Worth, A. J., McBride, M. & Rawls, W E.: Human papillomavirus and cervical intraepithelial neoplasia in women who subsequently had invasive cancer. Can. Med. As-Collins, J. E., Jenkins, D. & McCance, D. J.: Detection of human papillomavirus DNA sequences by in situ DNA-DNA hybridization in cervical intraepithelial neoplasia and invasive carcinoma: a retrospective study. J. Clin. Pathol. DeVilliers, E. M.: Heterogeneity of the human 135-151, 1989. SOC.

show abstract

The prevalence of human papillomavirus type 16 and 18 DNA in cervical cancer in different age groups: A study on the incidental cases of cervical cancer in Norway in 1983

Cited by 14 publications

References 30 publications

Implications of human papillomavirus type for survival in cervical squamous cell carcinoma

Implications of human papillomavirus type for survival in cervical squamous cell carcinoma

Younger age as a bad prognostic factor in patients with carcinoma of the cervix

The prevalence of human papillomavirus in cervical lesions with koilocytosis and/or CINI

Contact Info

Product

Resources

About