Implications of human papillomavirus type for survival in cervical squamous cell carcinoma

Hagmar, Björn; Christensen, Jette; Johansson, Bo; Kalantari, Mina; Ryd, Walter; Skyldberg, Barbro; Walaas, Lisa; Warleby, Bengt; Kristensen, Gunnar B.

doi:10.1046/j.1525-1438.1995.05050341.x

Cited by 15 publications

(6 citation statements)

References 18 publications

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“…Previous studies evaluated the relations between HPV genotype and cervical carcinoma prognosis and yielded inconsistent results 14–23. In contrast to our findings, Hagmar et al27 found that patients with HPV‐33 or HPV‐18‐associated tumors had worse prognoses than patients with other types of HPV infections. In their study, infection with double types was only detected in 5 (4.8%) of 107 patients with cervical carcinoma and patients with HPV‐16 infection were allocated to the group of HPV‐33‐associated tumors for survival analysis.…”

Section: Discussioncontrasting

confidence: 99%

Human papillomavirus‐31‐related types predict better survival in cervical carcinoma

Huang

Chao

Hwang³

2004

Cancer

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Section: Discussioncontrasting

confidence: 99%

Human papillomavirus‐31‐related types predict better survival in cervical carcinoma

Huang

Chao

Hwang³

2004

Cancer

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“…These observations further support the hypothesis that in HPV-related anorectal squamous cell carcinomas, the tumor suppressor function of the Rb protein is nullified even if it remains detectable immunohistochemically in a subset of the tumors. A number of studies have attempted to investigate whether HPV infection bears any prognostic significance in squamous cell carcinomas of the cervix (43)(44)(45)(46)(47)(48), penis (49), lung (50), and head and neck region (51,52). Although the accumulated data are inconclusive and controversial, it seems to be useful to separate tumors that are HPV related from those that are not because different etiopathogenetic pathways are involved.…”

Section: Discussionmentioning

confidence: 99%

Expression of p16, Rb, and p53 Proteins in Squamous Cell Carcinomas of the Anorectal Region Harboring Human Papillomavirus DNA

2003

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Human papillomavirus (HPV) has been implicated as an etiologic agent for the development of squamous cell carcinoma of the anorectal region. It has been shown that the HPV E6 and E7 oncoproteins are able to inactivate the tumor suppressor functions of p53 and Rb. In cervical and head and neck cancers, HPV infection is also associated with an overexpression of p16, a cyclin-dependent kinase inhibitor. The expression of these cell cycle regulators in squamous cell carcinomas of the anorectal region has not been well studied. In the current study, 29 cases of squamous cell carcinoma of the anorectal region were immunohistochemically examined for the expression of p16, Rb, and p53 proteins. Tumor cell DNA was also extracted from paraffin blocks and subjected to broad-spectrum HPV DNA testing and typing. The results show that the tumor cells exhibited a strong and diffuse nuclear stain (with some cytoplasmic positivity) for p16 in all 29 cases (100%). The adjacent nonneoplastic squamous epithelium or colonic mucosa, in contrast, was completely negative. Loss of Rb nuclear staining in tumor cells was observed in 20 cases (69%). The p53 protein was essentially undetectable, with only 6 cases containing <10% positive cells. HPV DNA was detected in every case (100%), with 25 cases (86%) harboring Type 16. In addition, almost identical results were obtained in 12 HPVpositive squamous cell carcinomas of the upper aerodigestive tract. This was in marked contrast to those of HPV-negative tumors, where positive p16 staining and loss of Rb expression were seen in only 2/21 (10%) and 1/21 (5%) cases, respectively. These observations indicate that overexpression of p16 and loss of Rb nuclear staining are commonly associated with high-risk HPV infection, which may serve as useful surrogate biomarkers for identifying squamous cell carcinomas harboring HPV DNA.

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“…For universal detection of HPV two sets of universal or consensus primer pairs from the highly conserved L1 region were used, one degenerated pair, My 11 and My 09, of Manos and coworkers (18), PCR procedures described in detail (19) and one nondegenerated pair, GP5ϩ and GP6ϩ of Husman et al (20), PCR procedures described in detail (5). Type-specific HPV primers for HPV 6, 16, 18, 31, and 33 from E5-E6-E7 gene regions were used according a procedure described earlier (21).…”

Section: Pcr Analyses For Hpvmentioning

confidence: 99%

Human Papillomavirus Infection, Centrosome Aberration, and Genetic Stability in Cervical Lesions

et al. 2001

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DNA replication and centrosome duplication have to be strictly synchronized to guarantee genomic stability. p53, pRb, cyclin E, and cyclin A are reported to be involved in the synchronizing process. We investigated the relationship between papillomavirus infection, centrosome aberration and aneuploidy during genesis of cervical carcinoma. The number of centrosomes found in cells from normal cervical epithelium (n ‫؍‬ 5), condyloma acuminata (n ‫؍‬ 5), cervical intraepithelial neoplasia (CIN) I, II, and III (n ‫؍‬ 14) and invasive cervical carcinoma (n ‫؍‬ 5) was analyzed by ␥ tubulin immunofluorescence staining. The nuclear DNA content was investigated by image cytometry and human papillomavirus (HPV) infection was determined by polymerase chain reaction. Normal epithelia and condyloma acuminata showed cells with one or two centrosomes, whereas CIN lesions showed cells with an increasing number of centrosomes. This abnormality was found to be lowest in CIN I lesions, increased with advancing grade of CIN and was highest in lesions of invasive carcinomas. In parallel, an increasing number of cells with aberrant DNA content was seen. All carcinomas and all except one of the CIN III lesions showed aneuploidy. Three CIN II cases were aneuploid and two cases with CIN I were tetraploid. Normal epithelia and condyloma acuminata showed diploidy. All invasive carcinomas and lesions with CIN were positive for high-risk HPV types 16, 18, or 31, except one invasive carcinoma and one CIN II lesion positive for universal primers only. Three condyloma acuminata were HPV 16-positive and one HPV 6-positive.The results suggest that high-risk HPV infection is correlated to a progressive numerical disturbance of centrosome replication followed by progressive chromosomal aberrations in CIN lesions and invasive carcinomas.

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Implications of human papillomavirus type for survival in cervical squamous cell carcinoma

Cited by 15 publications

References 18 publications

Human papillomavirus‐31‐related types predict better survival in cervical carcinoma

Human papillomavirus‐31‐related types predict better survival in cervical carcinoma

Expression of p16, Rb, and p53 Proteins in Squamous Cell Carcinomas of the Anorectal Region Harboring Human Papillomavirus DNA

Human Papillomavirus Infection, Centrosome Aberration, and Genetic Stability in Cervical Lesions

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