The Preparation of Micropropagated Plantlets for Transfer to Soil Without Acclimatization

Roberts, A. V.; Smith, E. F.; Mottley, J.

doi:10.1385/0-89603-161-6:227

Cited by 13 publications

(5 citation statements)

References 14 publications

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“…Before transplanting to the greenhouse, the normalized plants from all cultivars were transplanted to small pots containing peat-based soil and were acclimatized in the growth chamber for about one week, as hardening the plantlets at the transitions from in vitro to ex vitro is necessary to increase the survival of transplants [ 29 ]. In our study, all plantlets were observed to survive during the acclimatization period and maintained their survival rate when transferred to the greenhouse ( Figure 5 ).…”

Section: Resultsmentioning

confidence: 99%

Regeneration of Genetically Stable Plants from in Vitro Vitrified Leaves of Different Carnation Cultivars

Thu

Naing

Jeong

et al. 2020

Plants

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This study was conducted to investigate the efficacy of shoot regeneration from different leaf types (normal leaves and vitrified leaves) from three different carnation cultivars ‘Kumbuyl’, ‘Denev’, and ‘Jinju’ using different combinations of 3-indole butyric acid (IBA) and thidiazuron (TDZ) concentrations. The shoot tips cultured on Murashige and Skoog (MS) basal media (Type 1 media) produced normal leaves, while those cultured-on media supplemented with plant growth regulators and/or vitamin (Type 2 media and Type 3 media) produced vitrified leaves for all cultivars. Culture of normal leaf segments on MS medium containing different combinations of IBA and TDZ concentrations induced callus in all treatments; however, the callus was unable to induce shoots and finally became necrotic. In contrast, no callus induction was observed in the control (hormone-free treatment). When vitrified leaf segments underwent the same treatments, shoots were induced from the vitrified leaves (derived from Type 2 media) but were unhealthy and gradually died, whereas those induced from Type 3 media were vitrified and healthy. The optimal combination for the best shoot regeneration and number of shoots per explants varied depending on the genotypes used. The vitrified shoots induced from the leaves of Type 3 media transformed into normal shoots and survived well under greenhouse conditions. According to the results of random amplified polymorphic DNA (RAPD) analysis, the banding patterns of twelve primers that were detected in vitrified leaf-induced normalized shoots were identical to those of normal in vitro grown plants, indicating that no genetic variation had occurred during the procedure. Taken together, this study indicates that vitrified leaves can be used for shoot regeneration of recalcitrant carnation cultivars, regardless of the genotypes and types of vitrified leaves. However, as the number of shoots per explants was still low, further investigation is warranted to obtain a more efficient shoot regeneration protocol for genetic transformation of the cultivars.

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Section: Resultsmentioning

confidence: 99%

Regeneration of Genetically Stable Plants from in Vitro Vitrified Leaves of Different Carnation Cultivars

Thu

Naing

Jeong

et al. 2020

Plants

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“…Roberts et al (1990) found that placing the culture vessels on a cooled shelf could harden the plantlets. In the current study, we investigated the growth patterns of regenerants that were kept in growth chambers under a 16-h photoperiod at either 4~ or 24~ Many of the regenerants grew successfully at 4~ but died when held at 24~ This demonstrates the positive effect of a cold treatment before transplantation of H. orientalis plantlets.…”

Section: Discussionmentioning

confidence: 99%

Plant regeneration and morphology during in-vitro organogenesis fromHeloniopsis orientalis (Thunb.) C. Tanaka

2002

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Heloniopsis orientali$ (ILiliaceae) is an important horticultural crop native to Korea. Under natural conditions, germination is poor and plant growth is delayed. Therefore, we have developed a vegetative propagation method to produce plants with vigorous growth characteristics via tissue culture. Leaf tissues were cultured on MS basal media supplemented with growth regulators 2,4-D, TDZ, BA, or zeatin. The regenerated shoots were then initiated directly from leaf explants on an MS medium containing either 0.1 to 1.0 mg/L 2,4-D or 0.1 to 3.0 mg/I. BA. Healthy plantlets with adventitious roots were formed on the medium supplemented with 0.1 mg/L BA. TDZ triggered callus initiation without caulogenesis or rhizogenesis, and callus formation was better on the half-strength MS medium than on the full-strength medium. After the plants were acclimatized for one month at 4~ they were successfully transferred to soil. In addition, we used LM and SEM to investigate shoot morphogenesis at various stages of differentiation.

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“…When transferred to the field, plants grown in vitro were unable to interact with soil microbes or adjust to their surroundings [46]. It is vital to guarantee that the plants are planted in a moist environment for 2-4 weeks, depending on the rate of new leaf and root creation, in order to prolong life after transplantation to in vivo settings [47,48].…”

Section: Gladiolus In Vitro Propagation: Advantages and Applicationsmentioning

confidence: 99%

“…In vitro-grown plantlets were unable to engage with soil microbes and were also unable to cope with environmental conditions after field transfer [46]. In order to maximize survival after transplantation of plants to in vivo conditions, it is necessary to ensure that plantlets grow in a humid environment over a period of 2-4 weeks, depending on the rates at which new leaves and roots are produced [47,48]. According to one study, Cantor and Tolety [154] gladiolus could withstand temperatures as low as 5 • C and as high as 32 • C. As a result, acclimating outside is not advised.…”

Section: Ex Vitro Acclimatizationmentioning

confidence: 99%

In Vitro Propagation Journey of Ornamental Gladiolus (Gladiolus Species): A Systematic Review Analysis Based on More Than 50 Years Research

Kumar,

Chaudhary,

Sirohi

et al. 2024

Horticulturae

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Traditional gladiolus propagation methods are now supplemented with in vitro propagation to meet the demands of modern floriculture in terms of quick production of disease-free, quality planting material. Due to virus infections, vegetative propagation in gladiolus in the field is slow, and is a serious concern in the propagation of gladiolus. In vitro propagation provides an enormous increase in propagation rate and the ability to produce disease-free plant material. Numerous elements, including cultivars, explant type, size of explants, position of explants on medium, plant growth regulators and certain additives, incubation conditions, and sub-culturing time, all have a significant impact on in vitro clonal propagation of gladiolus plants as well as the development of in vitro cormel efficiency. There are certain obstacles and challenges that arise in the in vitro development of plants and the cormels of gladiolus. However, numerous studies and review reports on gladiolus for in vitro propagation have been reported, but very little is known about the factors influencing gladiolus’ in vitro effectiveness. In the present review, we focused on and analyzed research data accumulated over 50 years on diverse strategies for in vitro propagation such as direct, indirect organogenesis, and somatic embryogenesis, as well as various factors such as physical, nutritional, and hormonal influences on in vitro propagation, in vitro cormel formation efficiency, difficulties that arise, and new insights into in vitro development in gladiolus from the available literature worldwide. Future possibilities for further improvement in the in vitro propagation of ornamental gladiolus are also discussed. The current review provides insight into a comprehensive protocol for gladiolus in vitro propagation and emphasizes the importance of continuously advancing tissue culture techniques and factors influencing the in vitro efficiency towards improving in vitro plantlets and cormels in gladiolus (Gladiolus spp.).

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The Preparation of Micropropagated Plantlets for Transfer to Soil Without Acclimatization

Cited by 13 publications

References 14 publications

Regeneration of Genetically Stable Plants from in Vitro Vitrified Leaves of Different Carnation Cultivars

Regeneration of Genetically Stable Plants from in Vitro Vitrified Leaves of Different Carnation Cultivars

Plant regeneration and morphology during in-vitro organogenesis fromHeloniopsis orientalis (Thunb.) C. Tanaka

In Vitro Propagation Journey of Ornamental Gladiolus (Gladiolus Species): A Systematic Review Analysis Based on More Than 50 Years Research

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