Plant regeneration and morphology during in-vitro organogenesis fromHeloniopsis orientalis (Thunb.) C. Tanaka

Abstract

Heloniopsis orientali$ (ILiliaceae) is an important horticultural crop native to Korea. Under natural conditions, germination is poor and plant growth is delayed. Therefore, we have developed a vegetative propagation method to produce plants with vigorous growth characteristics via tissue culture. Leaf tissues were cultured on MS basal media supplemented with growth regulators 2,4-D, TDZ, BA, or zeatin. The regenerated shoots were then initiated directly from leaf explants on an MS medium containing either 0.1 to 1.0 mg/L 2,4-D or 0.1 to 3.0 mg/I. BA. Healthy plantlets with adventitious roots were formed on the medium supplemented with 0.1 mg/L BA. TDZ triggered callus initiation without caulogenesis or rhizogenesis, and callus formation was better on the half-strength MS medium than on the full-strength medium. After the plants were acclimatized for one month at 4~ they were successfully transferred to soil. In addition, we used LM and SEM to investigate shoot morphogenesis at various stages of differentiation.

Callus induction and plant regeneration from broccoli (Brassica oleracea var. italica) for transformation

“…Shoot regeneration from explants of leaves, cotyledons, hypocotyls, and protoplasts could prove useful for gene transfer via Agrobacterium (Cervera et al, 1998;Gonz~lez et al, 1998;Luo and }ia, 1998;Coll~n and Jarl, 1999;Cogan et al, 2001). Researchers (Cha et al, 2002;Thomas TD, 2002) require a means for comparing numerous variables in order to optimize the cell culture method. Researchers (Cha et al, 2002;Thomas TD, 2002) require a means for comparing numerous variables in order to optimize the cell culture method.…”

Underdeveloped Embryos and Dormancy Type in Seeds of Two Heloniopsis Species Endemic to Korea

“…The Asian species are mainly distributed in Japan, Taiwan, Korea, and southern Sakhalin (Fuse et al, 2004). Heloniopsis are typically evergreen perennial herbs that grow in moist, well-drained soils rich in organic matter under mesic temperate deciduous forests (Ahn and Lee, 1997;Cha et al, 2002;Kawano et al, 2007). These species have evergreen rosette leaves that turn purple in the winter and rose-purple, hermaphroditic flowers that bloom in the early spring with great potential for ornamental purposes (Kawano et al, 2007).…”