The demand for nature and ecotourism has been constantly rising last forty years and it will continue to grow. Due to the coronavirus, domestic tourism will increase exponentially, which would enhance ecotourism development. Foreigners and local tourists have become more aware to leave a positive impact on the environment where they travel and where they live. Serbia and Slovakia have a great potential to become attractive ecotourism destinations. Both countries are countries with a similar population number, no access to the sea, but with preserved nature, great gastronomic offer, and the main potential next to the capitals of Bratislava and Belgrade lies in ecotourism. At the same time, domestic ecotourism is a perfect economic activity that promotes sustainability and development. In the less developed regions, ecotourism can bring new employment opportunities and increase demand for local products. Agrotourism and rural tourism are inevitable part of sustainable development in Slovakia and Serbia. Despite of small territories of Slovakia and Serbia, both countries can offer a wide range of sceneries and natural beauties to be explored. This article aims to analyze the potential of ecotourism in the Slovak Republic and Serbia. To achieve the objective results, we’ve surveyed the foreign and local respondents. Our study also analyzes whether marketing strategies and promotion of domestic ecotourism is effective to attract tourists. The obtained results will be evaluated and compared. In the last part, the paper focuses on eco labels that currently exist in Serbia and Slovakia.