THe PossiBiLiTy of micRocHimeRism iDenTifying By HLA-A, B, c, DRB1, DQB1 TyPing B y PcR-ssP in THe moTHeRs AnD neonATes

Abstract

The article presents the possibility of microchimerism phenomenon detection in the genes of HLA loci A, B, C, DRB1, DQB1 by PCR-SSP in mothers and newborns with IUGR (11 pairs), hemolytic disease of the newborn (HDN) (9 pairs), after external rotation of the fetus (4 pairs) from women with malignancy (1 pair) and immune thrombocytopenia (1 pair). Out of 52 blood samples microchimerism was detected in 17 (33%) cases. In the group of mothers and children with HDN microchimerism was revealed in 9 (50%) samples and in the group with IUGR in 5 (23%). After the external rotation of fetus chimerism was observed in 1 (13%) case. In 2 (100%) samples of mother-child pair with autoimmune thrombocytopenia microchimerism was found in both samples, and in the couple child-mother with malignancy chimeric genes were not found. Chimeric genes were able to identify only at loci A, B, C, and DRB1 of HLA system. Chimerism in the DQB1 locus, was not observed in our samples.

Distribution of the major histocompatibility complex genes and haplotypes in newborns with the hemolytic disease and the intrauterine growth restriction

“…Одним из изучаемых в этой связи феноменов является фетальный микрохимеризм -явление циркуляции в крови матери до 1% клеток плода [4]. В рамках исследования микрохимеризма генов системы HLA у детей с патологией перинатального периода и их матерей [5,6] нами был проведен анализ генетических характеристик исследуемых групп.…”