The Polypeptide of Mr 14000 of Porcine Transmissible Gastroenteritis Virus: Gene Assignment and Intracellular Location

Garwes, D. J.; Stewart, Felicia H.; Britton, Paul

doi:10.1099/0022-1317-70-9-2495

Cited by 26 publications

(24 citation statements)

References 15 publications

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“…The PEDV ORF resembles the BCV I gene in coding for a high leucine content in the predicted products and having the ATG start codon in the same position with respect to that of the N gene. The viruses TGEV, PRCV, FIPV, FECV and CCV possess no such ORF, implying either that the I protein provides a non-essential function or that this function is provided by another protein, for example one encoded by a gene located downstream from the N gene in these viruses, which encodes a hydrophobic product of 78 amino acids (Garwes et al, 1989;Tung et al, 1992).…”

Section: Discussionmentioning

confidence: 99%

Sequence determination of the nucleocapsid protein gene of the porcine epidemic diarrhoea virus confirms that this virus is a coronavirus related to human coronavirus 229E and porcine transmissible gastroenteritis virus

Bridgen

Duarte

Tobler

et al. 1993

Journal of General Virology

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The nucleotide sequence of 1.7 kbp cDNA, comprising the region nearest the 3' end of the genome of the porcine epidemic diarrhoea virus (PEDV), has been independently determined for two European isolates of PEDV. Almost identical results were obtained for the two isolates, which were derived from cases of PEDV infection in Belgium and Britain in 1977 and 1987, respectively. The sequences contained a 1323 nucleotide (nt) open reading frame (ORF), which showed moderate identity to the nucleocapsid (N) gene of other coronaviruses. The greatest similarity at both the nucleic acid and protein levels was to the human coronavirus 229E.The PEDV N gene was, however, notably larger than that of the human 229E and porcine transmissible gastroenteritis viruses. This reflects the presence of a putative insertion of approximately 135 nt located towards the middle of the N gene. A second 336 nt ORF, which might encode a leucine-rich protein similar to, but shorter than, the bovine coronavirus internal protein was found within the PEDV N gene. Several RNA motifs typical of coronaviruses were also observed. These results confirm the earlier provisional classification of PEDV as a coronavirus.

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Section: Discussionmentioning

confidence: 99%

Sequence determination of the nucleocapsid protein gene of the porcine epidemic diarrhoea virus confirms that this virus is a coronavirus related to human coronavirus 229E and porcine transmissible gastroenteritis virus

Bridgen

Duarte

Tobler

et al. 1993

Journal of General Virology

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“…TGEV, feline coronavirus (FCoV) containing feline infectious peritonitis virus (FIPV) and canine coronavirus (CCoV) have Open Reading Frame 7 (ORF7) in the 3' end of the virus genome [3,8]. It has been reported that the TGEV ORF 7 encodes a 9.1-kDa hydrophobic protein, and this protein is associated with the endoplasmic reticulum and cell-surface membranes [4]. Its function, however, is not clear.…”

mentioning

confidence: 99%

Sequence Comparison of the ORF 7 Region of Transmissible Gastroenteritis Viruses Isolated in Japan

Taniguchi

Takahashi

et al. 2004

The Journal of Veterinary Medical Science

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ABSTRACT. The 3' end region nucleotide sequence, including ORF7, of nine Japanese and two U.S.A. isolates of transmissible gastroenteritis virus (TGEV) were determined and compared. Nine Japanese TGEV strains have been isolated over the past 40 years . From the comparison of determined nucleotide sequences, we could divide the TGEV Japanese isolates into two groups and d istinguish them from TGEV U.S.A. isolates. KEY WORDS: open reading frame 7, sequencing analysis, transmissible gastroenteritis virus.

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“…In this paper, we have adopted the nomenclature for coronavirus subgenomic mRNAs and viral genes suggested by the coronavirus study group (Cavanagh et al, 1990). Sequencing studies on TGEV FS772/70 showed that mRNA 2 encoded the S gene, mRNA 3 contained two potential open reading frames (ORFs) ORF-3a and ORF-3b 1989)] whose product has been identified in TGEV-and PRCV-infected cells (Garwes et aL, 1989). Sequence analysis showed that the potential product of the ORF3b gene from two virulent strains, FS772/70 and Miller (Wesley et al, 1989), had an Mr of 27600.…”

Section: Introductionmentioning

confidence: 99%

Sequence comparison of the 5' end of mRNA 3 from transmissible gastroenteritis virus and porcine respiratory coronavirus

Page¹,

Mawditt²,

Britton³

1991

Journal of General Virology

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The Polypeptide of Mr 14000 of Porcine Transmissible Gastroenteritis Virus: Gene Assignment and Intracellular Location

Cited by 26 publications

References 15 publications

Sequence determination of the nucleocapsid protein gene of the porcine epidemic diarrhoea virus confirms that this virus is a coronavirus related to human coronavirus 229E and porcine transmissible gastroenteritis virus

Sequence determination of the nucleocapsid protein gene of the porcine epidemic diarrhoea virus confirms that this virus is a coronavirus related to human coronavirus 229E and porcine transmissible gastroenteritis virus

Sequence Comparison of the ORF 7 Region of Transmissible Gastroenteritis Viruses Isolated in Japan

Sequence comparison of the 5' end of mRNA 3 from transmissible gastroenteritis virus and porcine respiratory coronavirus

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