Sequence comparison of the 5' end of mRNA 3 from transmissible gastroenteritis virus and porcine respiratory coronavirus

Page, Kevin W.; Mawditt, K.; Britton, Paul

doi:10.1099/0022-1317-72-3-579

Cited by 24 publications

(30 citation statements)

References 31 publications

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“…6). Deletions within the ORF 3 and 3-1 have been found in a small plaque (SP) TGEV, TGEV vaccine strain 188-SG, and PRCV [2,28,48,49]. The TGEV 188-SG strain contained a deletion of 250 nucleotides within the ORF-3a/3b (3 and 3-1) but had no deletion in the S gene.…”

Section: Discussionmentioning

confidence: 99%

Field Isolates of Transmissible Gastroenteritis Virus Differ at the Molecular Level from the Miller and Purdue Virulent and Attenuated Strains and from Porcine Respiratory Coronaviruses.

Kwon

Saif

Jackwood

1998

The Journal of Veterinary Medical Science

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ABSTRACT. The diversity in selected regions of the transmissible gastroenteritis virus (TGEV) and porcine respiratory coronavirus (PRCV) genomes was analyzed among known TGEV and PRCV strains and field isolates. The N-terminal half of the spike (S) glycoprotein gene and open reading frames (ORF) 3, 3-1 and 4 were amplified by reverse transcriptase reaction and polymerase chain reaction (RT/PCR), and analyzed using restriction fragment length polymorphism (RFLP) patterns of the amplified DNA. Reference TGEV strains (Miller and Purdue) and a PRCV strain (ISU-1), and TGEV and PRCV field isolates were analyzed. Based on the size of the ORF 3, 3-1 and 4 RT/PCR products, TGEV and PRCV strains could be quickly and easily differentiated into three groups designated TGEV Miller, Purdue types and PRCV. By RFLP analysis of the N-terminal region of the S glycoprotein gene and ORFs 3, 3-1 and 4, TGEV and PRCV strains were differentiated into five groups using the restriction enzyme Sau3AI. Sequence analysis of a PCR product in the ORFs 3, 3-1 and 4 from virulent and attenuated Miller strains demonstrated additional differences in that region which have been correlated with a change in virulence of TGEV isolates. -KEY WORDS: coronavirus, PRCV, RT/PCR-RFLP analysis, TGEV.

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Section: Discussionmentioning

confidence: 99%

Field Isolates of Transmissible Gastroenteritis Virus Differ at the Molecular Level from the Miller and Purdue Virulent and Attenuated Strains and from Porcine Respiratory Coronaviruses.

Kwon

Saif

Jackwood

1998

The Journal of Veterinary Medical Science

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“…Like FIPV and FECV, TGEV and PRCV are very similar antigenically across the S, M, and N proteins, though mAb analysis of their S proteins appears to offer a method for differentiating these two viruses (Callebaut, et al, 1988;Callebaut, et al, 1989). Genetic comparisons of PRCV and TGEV have revealed deletions in the PRCV S gene and the first NS gene downstream of S (Page, et al, 1991;Rasschaert, et al, 1990;Wesley, et al, 1991 ). Interestingly, analysis of a small plaque variant of TGEV also revealed alterations in the NS genes downstream of S (Wesley, et al, 1990).…”

Section: Clinical Aspects Of Coronavirus Infectionsmentioning

confidence: 99%

A review of feline infectious peritonitis virus: molecular biology, immunopathogenesis, clinical aspects, and vaccination

Olsen

1993

Veterinary Microbiology

111

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Feline infectious peritonitis (FIP) has been an elusive and frustrating problem for veterinary practitioners and cat breeders for many years. Over the last several years, reports have begun to elucidate aspects of the molecular biology of the causal virus (FIPV). These papers complement a rapidly growing base of knowledge concerning the molecular organization and replication of coronaviruses in general. The fascinating immunopathogenesis of FIPV infection and the virus' interaction with macrophages has also been the subject of several recent papers. It is now clear that FIPV may be of interest to scientists other than veterinary virologists since its pathogenesis may provide a useful model system for other viruses whose infectivity is enhanced in the presence of virus-specific antibody. With these advances and the recent release of the first commercially-available FIPV vaccine, it is appropriate to review what is known about the organization and replication of coronaviruses and the pathogenesis of FIPV infection.

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“…The molecular characterisation of a British isolate of PRCV has been undertaken with the aim of identifying differences between the PRCV and TGEV genomes which may be linked to the different tropisms and pathogenicities of the two viruses. Initial work found that: (1) the putative leader RNA sequences present on the viral RNA species, postulated to be involved in transcription of the mRNA species, were identical for TGEV and PRCV : (2) two of the PRCV mRNA species, 2 and 3, were smaller than the corresponding TGEV species Page et al, 1991): (3) sequencing studies on the PRCV genome, corresponding to the 5'-end of TGEV mRNA 3, identified several small deletions (84 nucleotides) resulting in the loss of the potential TGEV ORF-3a gene (Page et al, 1991). However, the 84 nucleotides deleted did not account for the size difference, about 600 nucleotides, observed between TGEV and PRCV mRNA 2 nor did the deletions explain the differential binding of the MAbs.…”

Section: Porcine Respiratory Coronavirus; Transmissible Gastroenteritmentioning

confidence: 99%

The cloning and sequencing of the virion protein genes from a British isolate of porcine respiratory coronavirus: comparison with transmissible gastroenteritis virus genes

Britton¹,

Mawditt²,

Page³

1991

Virus Research

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Previous analysis of porcine respiratory coronavirus (PRCV) mRNA species showed that mRNAs 2 and 3 were smaller than the corresponding transmissible gastroenteritis virus (TGEV) mRNA species (Page et al. (1991) J. Gen. Virol. 72, 579-587). Sequence analysis showed that mRNA 3 was smaller due to the presence of a new putative RNA-leader binding site upstream of the PRCV ORF-3 gene. However, this observation did not explain the deletion observed in PRCV mRNA 2. Polymerase chain reaction (PCR) was used to generate cDNA from the 3' coding region of the putative polymerase gene to the poly (A) tail of PRCV for comparison to the equivalent region from TGEV. The PRCV S protein was found to consist of 1225 amino acids, which had 98% similarity to the TGEV S protein. However, the PRCV S gene contained a 672 nucleotide deletion, corresponding to 224 amino acids (residues 21 to 245 in TGEV S protein), 59 nucleotides downstream of the S gene initiation codon. The PRCV genome from the ORF-3 gene to the poly (A) tail was sequenced for comparison to TGEV in order to identify other potential differences between the two viruses. Four ORFs were identified that showed 98% similarity to the TGEV ORF-4, M, N and ORF-7 genes. No other deletions or any PRCV specific sequences were identified.

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Sequence comparison of the 5' end of mRNA 3 from transmissible gastroenteritis virus and porcine respiratory coronavirus

Cited by 24 publications

References 31 publications

Field Isolates of Transmissible Gastroenteritis Virus Differ at the Molecular Level from the Miller and Purdue Virulent and Attenuated Strains and from Porcine Respiratory Coronaviruses.

Field Isolates of Transmissible Gastroenteritis Virus Differ at the Molecular Level from the Miller and Purdue Virulent and Attenuated Strains and from Porcine Respiratory Coronaviruses.

A review of feline infectious peritonitis virus: molecular biology, immunopathogenesis, clinical aspects, and vaccination

The cloning and sequencing of the virion protein genes from a British isolate of porcine respiratory coronavirus: comparison with transmissible gastroenteritis virus genes

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