The Platelet Releasate is Altered in Human Pregnancy

Szklanna, Paulina B.; Parsons, Martin; Wynne, Kieran; O’Connor, Heather; Egan, Karl; Allen, Seamus; Áinle, Fionnuala Ní; Maguire, Patricia B.

doi:10.1002/prca.201800162

Cited by 13 publications

(11 citation statements)

References 29 publications

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“…Increasing platelet count is one way to protect the body from the development of gestational diabetes in pregnant animals (Yang et al, 2015). It is known that physiological pregnancy is characterized by an increase in platelet activation and a decrease in the total number of circulating platelets (Szklanna et al, 2019). The value of thrombocrit in the pregnant rats increased, which is due to an increase in platelet count in the pregnant rats of group II.…”

Section: Discussionmentioning

confidence: 99%

Hematological parameters and protein metabolism in the blood of pregnant rats under the effect of vanadium citrate

Klymets

Iskra

Svarchevska

2021

Regul. Mech. Biosyst.

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Dose-dependent changes in protein metabolism in the blood and hematological parameters of pregnant rats under the effect of vanadium citrate are presented in the article. The animals were divided into five groups: group I – non-pregnant females, II – pregnant females consuming pure water without additives, III, IV, V – females which during the mating and pregnancy period received the solution of vanadium citrate at concentrations of 0.03, 0.125 and 0.50 μg V/mL water. The research findings show that in pregnant animals of group II, the level of urea and alkaline phosphatase activity increased, meanwhile aspartate aminotransferase activity decreased, as compared to the non-pregnant females of group І. The levels of total protein and albumin decreased; however, the content of β-globulins increased in the pregnant animals of group II, as compared with that in group I. Also, in the rats of group II, there was a decrease in hemolysis time, total content of erythrocytes and hemoglobin, the content of old and mature erythrocytes, while the content of young erythrocytes increased, as compared to group I. The platelet content and thrombocrit in rats of group II increased in comparison with group I. The content of leukocytes and lymphocytes in pregnant animals of group II decreased, while the content of granulocytes increased, in contrast to non-pregnant rats. Under the effect of vanadium citrate at concentrations of 0.03–0.50 μg V/mL, there was a significant increase in the maximum number of prohemolized erythrocytes, the time of maximum hemolysis was delayed by 0.4–0.6 min, as compared with the pregnant rats of group II. This did not affect the time of total hemolysis in rats of groups III and V, as compared with the pregnant animals in group II. Under the effect of vanadium citrate, an increase in the content of young erythrocytes was observed, as compared with group II. The hemoglobin content decreased at the concentration of 0.125 μg V/mL, while at the concentration of 0.50 μg V/mL it increased, as compared to the pregnant animals of group II. Also, under the effect of vanadium citrate there was a decrease in the mean hemoglobin concentration in the erythrocyte. In pregnant animals fed with vanadium citrate solutions, the platelet content and thrombocrit, the relative width of platelet distribution by volume decreased, as compared with the pregnant rats of group II. The content of leukocytes, lymphocytes and granulocytes under the effect of vanadium citrate increased, as compared with the pregnant animals in group II. Under the effect of vanadium citrate at the concentration of 0.03 μg V/mL, the level of albumin, creatinine and aspartate aminotransferase activity increased in blood plasma in comparison with group II. Meanwhile, at the concentration of 0.125 μg V/mL, the relative content of γ-globulins and aspartate aminotransferase activity increased, alkaline phosphatase activity and urea level decreased in comparison with group II. However at the concentration of 0.50 μg V/mL, the relative α- and γ-globulins content and aspartate aminotransferase activity increased, at the same time, the relative β-globulins content and urea level decreased in comparison with group II. Therefore, vanadium citrate normalizes the indicators of protein metabolism during pregnancy, thus it can be considered as a potential dietary drug for the pregnant.

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Section: Discussionmentioning

confidence: 99%

Hematological parameters and protein metabolism in the blood of pregnant rats under the effect of vanadium citrate

Klymets

Iskra

Svarchevska

2021

Regul. Mech. Biosyst.

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“…Intact platelets and the platelet clot were carefully removed by centrifuging sequentially x3 at 1000 × g for 10 min at 4°C (in the presence of 2% protease and 2% phosphatase inhibitors; Roche) and harvesting the supernatant each time. The final spin yielded the activated PR as before ( 7 , 16 , 21 , 22 ), which was stored at −80 °C until further use.…”

Section: Methodsmentioning

confidence: 99%

Comparative Platelet Releasate Proteomic Profiling of Acute Coronary Syndrome versus Stable Coronary Artery Disease

Maguire

Parsons

Szklanna

et al. 2020

Front. Cardiovasc. Med.

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Upon activation, platelets release a host of soluble and vesicular signals, collectively termed the “platelet releasate” (PR). The contents of this PR play a significant role in haemostasis, inflammation, and pathologic sequelae . Despite this, proteomic studies investigating the PR in coronary artery disease have not been performed. Here, we undertook a comparative label-free quantitative (LFQ) proteomic profiling of the 1 U/ml thrombin-induced PR from 13 acute coronary syndrome vs. 14 stable angina pectoris patients using a tandem mass spectrometry approach. Data are available via ProteomeXchange with identifier PXD009356. 318 PR proteins were identified across both cohorts with 9 proteins found to be differentially released, including tetranectin (CLEC3B), protein disulfide-isomerase-A3 (PDIA3), coagulation factor V (F5), and fibronectin (FN1). Strikingly, these 9 differential proteins were all associated with the gene ontology cellular component term “extracellular vesicle” and reduced levels of EVs were detected in the corresponding plasma of ST-segment elevation myocardial infarction (STEMI) patients. Network analysis revealed 3 proteins either reduced (F5; FN1) or absent (CLEC3B) in the PR of STEMI patients that are strongly connected to both the clotting cascade and major druggable targets on platelets. This moderated proteomic signature may prove useful for non-invasive risk assessment of the progression of coronary artery disease. These data further contribute to the growing evidence-base of using the platelet releasate as a predictor of pathological state and disease severity.

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“…84,85 Indeed, recent data have demonstrated that specific proteomic and genomic EV profiles discriminate between various physiological and pathological states (e.g., pregnant vs. non-pregnant state) and as such may represent an avenue for novel diagnostics/predictive models in different diseases. [86][87][88][89] The contents of distinct EV populations not only have the potential to act as a 'barcode' for disease but also mediate important signalling activities and have been shown to regulate numerous physiological and pathological processes. 85,[90][91][92] Specific EV profiles have been described in preeclampsia and appear to play an important role in the progression of this disorder.…”

Section: Derangements Of Blood Coagulation and Fibrinolysis In Preeclmentioning

confidence: 99%

Pathophysiology of the Venous Thromboembolism Risk in Preeclampsia

et al. 2020

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SummaryHaemostatic derangements are a hallmark of preeclampsia and appear to favour an increased risk of venous thromboembolism. However, haemorrhagic complications have also been reported. The mechanisms underlying these competing risks remain to be fully elucidated although recent work has highlighted the role of placental factors, such as extra-cellular vesicles and inflammatory mediators, in modulating these haemostatic derangements as well as driving progression of preeclampsia. Identifying affected women at risk of thrombosis and managing the competing thrombotic and haemorrhagic risks continue to be a significant clinical challenge. Derangements in blood coagulation are also implicated in the pathogenesis of preeclampsia; however, the role of antiplatelet or anticoagulant drugs in the management of this disorder remains a source of debate. Further characterisation of the underlying molecular mechanisms would likely be of major translational relevance and could provide insights into the pathogenesis of this disease as well as the associated haemostatic dysfunction.

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The Platelet Releasate is Altered in Human Pregnancy

Cited by 13 publications

References 29 publications

Hematological parameters and protein metabolism in the blood of pregnant rats under the effect of vanadium citrate

Hematological parameters and protein metabolism in the blood of pregnant rats under the effect of vanadium citrate

Comparative Platelet Releasate Proteomic Profiling of Acute Coronary Syndrome versus Stable Coronary Artery Disease

Pathophysiology of the Venous Thromboembolism Risk in Preeclampsia

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