The Pex3–Inp1 complex tethers yeast peroxisomes to the plasma membrane

Hulmes, Georgia E.; Hutchinson, John D; Dahan, Noa; Nuttall, James; Allwood, Ellen G.; Ayscough, Kathryn R.; Hettema, Ewald H.

doi:10.1083/jcb.201906021

Cited by 14 publications

(14 citation statements)

References 40 publications

(66 reference statements)

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“…The peroxisome-ER contact site generated by Inp1 and Pex3 is required to retain peroxisomes in the mother cell. Two recent studies contradicted the "molecular hinge" model as the new model showed that Inp1 is a component of the tether at the peroxisome-plasma membrane contact site (Hulmes et al, 2020;Krikken et al, 2020). The N-terminal 100 amino acids of ScInp1 localizes to the plasma membrane possibly by binding to PI-(4,5)-P2.…”

Section: Peroxisome-plasma Membrane Contact Sitesmentioning

confidence: 95%

“…However, the loss of ScPex30 does not have any effect on the retention of peroxisomes. If the ER plays a role along with Inp1 in peroxisome retention, there might be proteins similar to ScPex30 that are required for the correct positioning of peroxisomes (Hulmes et al, 2020) (Figure 2C). Interestingly, another study also showed that OpInp1 is required for the formation of peroxisome-plasma membrane contact and peroxisome retention.…”

Section: Peroxisome-plasma Membrane Contact Sitesmentioning

confidence: 99%

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Peroxisomal Membrane Contact Sites in Yeasts

Joshi

2021

Front. Cell Dev. Biol.

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Peroxisomes are ubiquitous, single membrane-bound organelles that play a crucial role in lipid metabolism and human health. While peroxisome number is maintained by the division of existing peroxisomes, nascent peroxisomes can be generated from the endoplasmic reticulum (ER) membrane in yeasts. During formation and proliferation, peroxisomes maintain membrane contacts with the ER. In addition to the ER, contacts between peroxisomes and other organelles such as lipid droplets, mitochondria, vacuole, and plasma membrane have been reported. These membrane contact sites (MCS) are dynamic and important for cellular function. This review focuses on the recent developments in peroxisome biogenesis and the functional importance of peroxisomal MCS in yeasts.

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Section: Peroxisome-plasma Membrane Contact Sitesmentioning

confidence: 95%

Section: Peroxisome-plasma Membrane Contact Sitesmentioning

confidence: 99%

Peroxisomal Membrane Contact Sites in Yeasts

Joshi

2021

Front. Cell Dev. Biol.

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“…For example, some proteins, such as Pex3, Pex12, Pex27, and Hem14, are not evenly distributed but rather seem to compartmentalize on specific sites of the membrane. While Pex3 was previously shown to compartmentalize on the membrane and form contact sites with the plasma membrane (Hulmes et al, 2020) and the vacuole (Wu et al, 2019), the other proteins were never suggested to have such a distribution. It will be interesting to further study whether this distribution is due to their function in membrane contact sites.…”

Section: High-resolution Imaging Reveals the Sub-organellar Distribution Of The Peroxi-omementioning

confidence: 99%

Systematic multi-level analysis of an organelle proteome reveals new peroxisomal functions

Yifrach

Holbrook-Smith

Bürgi

et al. 2021

Preprint

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Seventy years following the discovery of peroxisomes, their proteome remains undefined. Uncovering the complete peroxisomal proteome, the peroxi-ome, is crucial for understanding peroxisomal activities and cellular metabolism. We used high- content microscopy to uncover the peroxi-ome of the model eukaryote – Saccharomyces cerevisiae. This strategy enabled us to expand the known organellar proteome by ∼40% and paved the way for performing systematic, whole-organellar proteome assays. Coupled with targeted experiments this allowed us to discover new peroxisomal functions. By characterizing the sub-organellar localization and protein targeting dependencies into the organelle, we unveiled non-canonical targeting routes. Metabolomic analysis of the peroxi-ome revealed the role of several newly-identified resident enzymes. Importantly, we found a regulatory role of peroxisomes during gluconeogenesis, which is fundamental for understanding cellular metabolism. With the current recognition that peroxisomes play a crucial part in organismal physiology, our approach lays the foundation for deep characterization of peroxisome function in health and disease.

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“…The formation of protein tethers at the mother cell cortex facilitates organelle retention, whereas the association with the actin-dependent myosin V motor Myo2 promotes traffic to the daughter cell of both peroxisomes and mitochondria [ 80 ]. Peroxisomes anchor on the mother cell cortex by forming a tether between Inp1 and peroxisomal Pex3 [ 81 , 82 , 83 , 84 ]. Organelle fission facilitates traffic by the subsequent attachment to Myo2, mediated by Inp2 [ 85 ].…”

Section: Peroxisome and Mitochondrial Dynamics Are Involved In Asementioning

confidence: 99%

Dynamic Regulation of Peroxisomes and Mitochondria during Fungal Development

Navarro-Espíndola

Suaste-Olmos

Peraza-Reyes

2020

JoF

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Peroxisomes and mitochondria are organelles that perform major functions in the cell and whose activity is very closely associated. In fungi, the function of these organelles is critical for many developmental processes. Recent studies have disclosed that, additionally, fungal development comprises a dynamic regulation of the activity of these organelles, which involves a developmental regulation of organelle assembly, as well as a dynamic modulation of the abundance, distribution, and morphology of these organelles. Furthermore, for many of these processes, the dynamics of peroxisomes and mitochondria are governed by common factors. Notably, intense research has revealed that the process that drives the division of mitochondria and peroxisomes contributes to several developmental processes—including the formation of asexual spores, the differentiation of infective structures by pathogenic fungi, and sexual development—and that these processes rely on selective removal of these organelles via autophagy. Furthermore, evidence has been obtained suggesting a coordinated regulation of organelle assembly and dynamics during development and supporting the existence of regulatory systems controlling fungal development in response to mitochondrial activity. Gathered information underscores an important role for mitochondrial and peroxisome dynamics in fungal development and suggests that this process involves the concerted activity of these organelles.

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The Pex3–Inp1 complex tethers yeast peroxisomes to the plasma membrane

Cited by 14 publications

References 40 publications

Peroxisomal Membrane Contact Sites in Yeasts

Peroxisomal Membrane Contact Sites in Yeasts

Systematic multi-level analysis of an organelle proteome reveals new peroxisomal functions

Dynamic Regulation of Peroxisomes and Mitochondria during Fungal Development

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