Peroxisomal Membrane Contact Sites in Yeasts

Joshi, Amit

doi:10.3389/fcell.2021.735031

Cited by 8 publications

(4 citation statements)

References 71 publications

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“…3, the discrete SSD5 structures associate with the ER. This agrees with the general association between peroxisomes and ER (Joshi, 2021), which we con rmed by co-expressing YFP-SSD5, mCherry-SKL and SP-CFP-HDEL (Fig. 4B and Movie S1).…”

Section: Ssd5 Is a Cytosolic Protein Attracted To A Peroxisomal Subdo...supporting

confidence: 90%

A plant peroxisome lipase, SSD5, is required for steryl ester accumulation and activation of autoimmunity

Xie

Feng

et al. 2023

Preprint

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Excess cellular sterol is harmful in mammals and plants, but the mechanisms why are awaiting clarification. Here we find a strong autoimmune response to be associated to excess endoplasmic reticulum (ER) sterols. This was obtained by studying a plant peroxisome lipase, SSD5, required for the lesion phenotype of the Arabidopsis syntaxin mutant, pen1 syp122. SSD5 is a lipase with a catalytic triad including a GxSxG motif localized to a subdomain of the peroxisome periphery. Lipidomics revealed reduced steryl ester levels in pen1 syp122 when SSD5 is mutated. This involvement in sterol homeostasis was confirmed by a requirement of SSD5 for the lesions of hise1 psat1 that suffers from excess ER sterol. These data suggest SSD5 is contributing to a peroxisome-located segment of the sterol biosynthesis pathway. SSD5’s contribution to the pen1 syp122 autoimmunity is not associated with nine highly diverse down-stream immune components, and SSD5 does not influence general plant disease levels and immunity. Therefore, our data indicated SSD5 as well as ER sterol functions up-stream of immune activation. This in turn suggests plant excess ER sterol to activate one or more immune receptors.

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Section: Ssd5 Is a Cytosolic Protein Attracted To A Peroxisomal Subdo...supporting

confidence: 90%

A plant peroxisome lipase, SSD5, is required for steryl ester accumulation and activation of autoimmunity

Xie

Feng

et al. 2023

Preprint

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show abstract

“…Furthermore, the small GTPase Arf1 localises to contacts between peroxisomes, mitochondria, and LDs in yeast, regulating the flow of fatty acids from LDs to mitochondria/peroxisomes and ultimately controlling mitochondrial function (Enkler et al 2023 ) (see “ Mysterious functions: peroxisomal metabolism updated ”). Finally, whilst peroxisome-mitochondria MCSs have been characterised in yeast (Mattiazzi Ušaj et al 2015 ; Shai et al 2018 ; Joshi 2021 ) (Fig. 4 ) their composition and regulation remains more mysterious in mammals.…”

Section: Progress Towards Understanding the Mysteries Of Peroxisome-o...mentioning

confidence: 99%

“…MCS proteins are mammalian unless otherwise stated, and those in bold are addressed further in the text. Adapted from Silva et al ( 2020) Ušaj et al 2015;Shai et al 2018;Joshi 2021) (Fig. 4) their composition and regulation remains more mysterious in mammals.…”

Section: Peroxisome-mitochondria Mcssmentioning

confidence: 99%

The peroxisome: an update on mysteries 3.0

Kumar,

Islinger,

Worthy

et al. 2024

Histochem Cell Biol

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Peroxisomes are highly dynamic, oxidative organelles with key metabolic functions in cellular lipid metabolism, such as the β-oxidation of fatty acids and the synthesis of myelin sheath lipids, as well as the regulation of cellular redox balance. Loss of peroxisomal functions causes severe metabolic disorders in humans. Furthermore, peroxisomes also fulfil protective roles in pathogen and viral defence and immunity, highlighting their wider significance in human health and disease. This has sparked increasing interest in peroxisome biology and their physiological functions. This review presents an update and a continuation of three previous review articles addressing the unsolved mysteries of this remarkable organelle. We continue to highlight recent discoveries, advancements, and trends in peroxisome research, and address novel findings on the metabolic functions of peroxisomes, their biogenesis, protein import, membrane dynamics and division, as well as on peroxisome–organelle membrane contact sites and organelle cooperation. Furthermore, recent insights into peroxisome organisation through super-resolution microscopy are discussed. Finally, we address new roles for peroxisomes in immune and defence mechanisms and in human disorders, and for peroxisomal functions in different cell/tissue types, in particular their contribution to organ-specific pathologies.

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“…This can be in part attributed to the ability of yeasts to rapidly switch their metabolism from glucose‐rich to glucose‐depleted (glucose starvation) conditions, or use FAs such as oleate for energy production via FA β‐oxidation. This fast response to metabolic rewiring made it easy to study contact site dynamics between mitochondria and the ER [73,74], vacuoles [75,76] and peroxisomes [28,77]. However, little is known about the contacts between mitochondria and LDs in yeast.…”

Section: Bridging Lds and Mitochondriamentioning

confidence: 99%

Functional interplay of lipid droplets and mitochondria

Enkler,

Spang

2024

FEBS Letters

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Our body stores energy mostly in form of fatty acids (FAs) in lipid droplets (LDs). From there the FAs can be mobilized and transferred to peroxisomes and mitochondria. This transfer is dependent on close opposition of LDs and mitochondria and peroxisomes and happens at membrane contact sites. However, the composition and the dynamics of these contact sites is not well understood, which is in part due to the dependence on the metabolic state of the cell and on the cell‐ and tissue‐type. Here, we summarize the current knowledge on the contacts between lipid droplets and mitochondria both in mammals and in the yeast Saccharomyces cerevisiae, in which various contact sites are well studied. We discuss possible functions of the contact site and their implication in disease.

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Peroxisomal Membrane Contact Sites in Yeasts

Cited by 8 publications

References 71 publications

A plant peroxisome lipase, SSD5, is required for steryl ester accumulation and activation of autoimmunity

A plant peroxisome lipase, SSD5, is required for steryl ester accumulation and activation of autoimmunity

The peroxisome: an update on mysteries 3.0

Functional interplay of lipid droplets and mitochondria

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