The partial characterization of the antibacterial peptide bacteriocin G2 produced by the probiotic bacteria Lactobacillus plantarum G2

Abstract

The aim of this study was the partial characterization of the antimicrobial peptide bacteriocin G 2 produced by probiotic bacteria Lactobacillus plantarum G 2 , which was isolated from a clinical sample of a healthy person. Antimicrobial substance was secreted in the supernatant of an L. plantarum G 2 culture, and showed a diverse spectrum of antimicrobial activity of all the tested strains of the genera Lactobacillus and the pathogenic bacteria Staphylococcus aureus and Salmonella аbony. Isoelectric focusing revealed that bacteriocin G 2 is a cationic peptide (pI about 10) with a molecular mass of 2.2 kDa according to tricine-sodium dodecyl sulphate-polyacrylamide gel electrophoresis, SDS-PAGE. The antimicrobial activity of bacteriocin G 2 was diminished by the proteolytic action of trypsin and proteinase K. Bacteriocin G 2 preserved its biological activity in the temperature range 40-60 °C (15 min), which was lost at 80 °C. Bacteriocin G 2 was stable in the pH range 2-9, while treatment with 1 % Tween 80 and 1 % urea resulted in increased antimicrobial activity. The probiotic strain L. plantarum G 2 produces the antimicrobial substance proteinaceous in nature with bacteriocin characteristics. Bacteriocin production is one of the key properties of probiotic bacteria with clinical potential as antiinfective agents, which will increase the likelihood of its in vivo efficacy.

Partial Characterization of Bacteriocins from Two <i>Pediococcus acidilactici</i> Strains Isolated during Traditional Sorghum Beer Processing in Côte d’Ivoire

“…So these strains may be used as potential probiotic to produce tchapalo and lots of other fermented cereal beverages. Indeed, many bacteriocins and bacteriocin-like substances are considerably more tolerant to acid than alkaline pH values [23] [26] [29]. Similar results were reported by Mandal et al [6].…”

Cytotoxicity and antibacterial activity of gold-supported cerium oxide nanoparticles

“…[14][15][16] Of these metabolites, bacteriocins are of particular interest because there have been several reports demonstrating their antimicrobial efficiency across a broad spectrum of microbes. [17][18][19] Although many bacteria can produce bacteriocins, L. plantarum is chosen in this study due to its Generally Regarded As Safe status. Bacteriocins are proteinaceous antimicrobial compounds produced extracellularly during the stationary phase of bacterial culture.…”

“…15,17 Bacteriocins produced by Lactobacillus species are known as "plantaricins", and their stability varies depending on the specific physicochemical environment and Lactobacillus species used. 14,[19][20][21] In view of this, we intended to coculture each test bacteria with L. plantarum to evaluate the possible synergistic antibacterial effect by L. plantarum and as-synthesized CeO 2 NPs or Au/CeO 2 NPs. Presently, there are no reports available on determining the antibacterial efficacy of Au/ CeO 2 NPs in a coculture where the culture has more than one bacterial species.…”

The probiotic, Leuconostoc mesenteroides, inhibits Listeria monocytogenes biofilm formation

“…Probiotics are beneficial microorganisms that improve host immunity (Hill et al, 2014), and certain probiotics such as Lactobacillus species outcompete pathogens by secreting bacteriocins, which are proteinaceous or peptidic toxins that inhibit the growth of similar or closely related bacterial strains (Mukherjee & Ramesh, 2015;Šeatovi c et al, 2011). Lactobacillus probiotics inhibit Salmonella enterica serotype Typhimurium and L. monocytogenes biofilm formation (Woo & Ahn, 2013), and also impede the biofilms of Candida albicans by decreasing their growth, adhesion, and filamentation (Matsubara et al, 2016).…”