The probiotic, <scp><i>Leuconostoc mesenteroides</i></scp>, inhibits <scp><i>Listeria monocytogenes</i></scp> biofilm formation

Shao, Xiaofeng; Fang, Kuili; Medina, Daniel A.; Wan, Jason; Lee, Jung‐Lim; Hong, Seok Hoon

doi:10.1111/jfs.12750

Cited by 27 publications

(7 citation statements)

References 43 publications

(73 reference statements)

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“…It is reported that L. sakei from Kimchi are promising anti-noroviral candidates [37]. Studies have shown that L. sakei CRL1862 and L. mesenteroides Com75 could be environmentally friendly agents against foodborne pathogens [38,39]. No study or evidence has however demonstrated that the intake of probiotics may have potential cariogenic or cariostatic effects.…”

Section: Isolation and Identification Of Potentially Cariogenic Microorganismsmentioning

confidence: 99%

Isolation and Identification of Potentially Pathogenic Microorganisms Associated with Dental Caries in Human Teeth Biofilms

et al. 2020

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Dental caries is attributed to the predominance of cariogenic microorganisms. Cariogenic microorganisms are pathological factors leading to acidification of the oral microenvironment, which is related to the initiation and progression of caries. The accepted cariogenic microorganism is Streptococcus mutans (S. mutans). However, studies have found that caries could occur in the absence of S. mutans. This study aimed to assess the presence of potentially cariogenic microorganisms in human teeth biofilm. The microorganisms were isolated from human mouth and freshly extracted human maxillary incisors extracted for reasons of caries. The isolates were sorted based on their acidogenic and aciduric properties, and the S. mutans was used as the reference strain. Four potentially cariogenic strains were selected. The selected strains were identified as Streptococcus salivarius (S. salivarius), Streptococcus anginosus (S. anginosus), Leuconostoc mesenteroides (L. mesenteroides), and Lactobacillus sakei (L. sakei) through morphological analysis followed by 16S rRNA gene sequence analysis. The cariogenicity of isolates was analyzed. We show, for the first time, an association between L. sakei (present in fermented food) and dental caries. The data provide useful information on the role of lactic acid bacteria from fermented foods and oral commensal streptococci in dental caries.

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Section: Isolation and Identification Of Potentially Cariogenic Microorganismsmentioning

confidence: 99%

Isolation and Identification of Potentially Pathogenic Microorganisms Associated with Dental Caries in Human Teeth Biofilms

et al. 2020

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“…Interestingly, previous work has found the supernatant from an isolate of L. lactis to have antimicrobial effects on several pathogenic species including Staphylococcus aureus, Escherichia coli O157, and Proteusbacillus vulgaris (Zhang et al, 2013). Similarly, other members of the genera Leuconostoc and Weissella have been shown to have antimicrobial properties, most notably by reducing the formation of biofilms (Quattrini et al, 2020;Shao et al, 2020;Jang et al, 2021). It is therefore plausible that the high copper treatment exhibited lower virulence because it caused species sorting towards non-pathogenic species, and that this affect was possibly compounded by the resulting species potentially having antimicrobial traits.…”

Section: Discussionmentioning

confidence: 99%

Copper reduces the virulence of bacterial communities at environmentally relevant concentrations

Lear

Padfield

Hesse

et al. 2023

Preprint

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Increasing environmental concentrations of metals as a result of anthropogenic pollution are significantly changing many microbial communities. While there is evidence metal pollution can result in increased antibiotic resistance, the effects of metal pollution on virulence remains largely undetermined. Here, we experimentally test whether metal stress alters the virulence of bacterial communities. We do this by incubating three wastewater influent communities under different environmentally relevant copper concentrations for three days. We then quantify the virulence of the community using the Galleria mellonella infection model, and test if differences are due to changes in the rate of biomass accumulation (productivity), copper resistance, or community composition (quantified using 16S amplicon sequencing). The virulence of the communities was found to be reduced by the highest copper concentration, but not to be affected by the lower concentration. As well as reduced virulence, communities exposed to the highest copper concentration were less diverse and had lower productivity. This work highlights that metal pollution may decrease virulence in bacterial communities, but at a cost to diversity and productivity.

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“…Several Ln. mesenteroides strains are reported to have anti-listerial, antiviral, or immunomodulatory activities (Seo et al, 2012;Shao et al, 2020). Since the production of exopolysaccharides and bacteriocins are important properties of Ln.…”

Section: Discussionmentioning

confidence: 99%

Isolation of a Leuconostoc mesenteroides Strain With Anti-Porcine Epidemic Diarrhea Virus Activities From Kefir Grains

et al. 2020

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Swine grown under commercial conditions are vulnerable to environmental exposure to several viruses, which may cause infectious diseases and spread easily and rapidly, resulting in significant economic losses in animal husbandry. Previous studies have suggested that probiotics seem to be a new and promising alternative to vaccinations to protect animals against potential viral infections. In this study, we used the Vero cell culture model of infection to study porcine epidemic diarrhea virus (PEDV). We screened lactic acid bacteria (LAB) with anti-PEDV potential from kefir grains, which are starter cultures used to ferment milk into kefir. Twenty-nine LAB strains were isolated and identified as Enterococcus durans, Lactobacillus kefiri, Lactococcus lactis, and Leuconostoc mesenteroides, according to 16S ribosomal RNA (rRNA) and rpoA gene sequence analyses. The anti-PEDV activities of the LAB intracellular extracts were compared, and the intracellular extracts of Ln. mesenteroides showed higher anti-PEDV activities than that of the other species. Among the Ln. mesenteroides strains, a strain designated YPK30 showed a higher growth rate than that of the other strains and was further evaluated for its anti-PEDV activity. The results showed that the intracellular extracts of Ln. mesenteroides YPK30 possessed in vitro prophylactic, therapeutic, and direct-inhibitory effects against PEDV in the Vero cell model. The expression levels of Type 1 interferon (IFN)-dependent genes, including Myxovirus resistance 1 (MX1) and interferon-stimulated gene 15 (ISG15), were significantly increased after treatment with intracellular extracts of Ln. mesenteroides YPK30 for 24 h. Such expression suggests that the anti-PEDV activity of Ln. mesenteroides YPK30 could be attributed to its up-regulatory effect on the expression of MX1 and ISG15 genes. These results suggested that Ln. mesenteroides YPK30 has the potential to provide some levels of host protection against PEDV infections.

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The probiotic, Leuconostoc mesenteroides, inhibits Listeria monocytogenes biofilm formation

Cited by 27 publications

References 43 publications

Isolation and Identification of Potentially Pathogenic Microorganisms Associated with Dental Caries in Human Teeth Biofilms

Isolation and Identification of Potentially Pathogenic Microorganisms Associated with Dental Caries in Human Teeth Biofilms

Copper reduces the virulence of bacterial communities at environmentally relevant concentrations

Isolation of a Leuconostoc mesenteroides Strain With Anti-Porcine Epidemic Diarrhea Virus Activities From Kefir Grains

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