Isolation and Identification of Potentially Pathogenic Microorganisms Associated with Dental Caries in Human Teeth Biofilms

Chen, Xiuqin; Daliri, ‪Eric Banan-Mwine; Chelliah, Ramachandran; Oh, Deog‐Hwan

doi:10.3390/microorganisms8101596

Cited by 19 publications

(11 citation statements)

References 45 publications

(54 reference statements)

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“…In the mouth, this frequent exposure to low pH will favour not only intrinsically aciduric species, but also bacteria capable of eliciting an acid-tolerance response. Accordingly, the adapted bacteria and intrinsically acid-tolerant ones will survive in a lethal pH (pH 2.8) in vitro, while non-acid-tolerant bacteria will not [53,54]. In the present study, it was observed that isolates from the caries-active and the caries-free groups presented a variable aciduricity when subjected to extreme acidity conditions (pH 2.8) and only managed to survive in this environment for 30 min.…”

Section: Discussionmentioning

confidence: 50%

Acidogenic and aciduric properties of Streptococcus mutans serotype c according to its genomic variability

Bedoya-Correa

Rincón‐Rodríguez

Parada-Sanchez

2021

European J Oral Sciences

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Streptococcus mutans (S. mutans) has a wide genetic diversity that contributes to its phenotypic heterogeneity, and may be related to attributes associated with acidogenicity and aciduricity. The aim of this study was to evaluate the acidogenic and aciduric properties of S. mutans serotype c isolates from saliva of schoolchildren according to the genomic variability. S. mutans isolates were identified by polymerase chain reaction. Fifty S. mutans serotype c isolates were genotyped by pulsed field gel electrophoresis and tested for their ability to produce and resist acid. Three specific genotypes were identified in the caries-active group and only one in the caries-free group. Although isolates were similarly acidogenic, an exclusive cariesactive genotype had the greatest glycolytic activity. In contrast, isolates exhibited variable aciduricity, and three caries-active genotypes were the least aciduric. We concluded that there is genetic variability within serotype c. Acid production was similar regardless of the caries status but correlated with the number of genotypes. In addition, resistance to acid could be an important characteristic for the establishment and colonisation of specific genotypes in children with caries. However, it is important to evaluate children's intrinsic characteristics and other phenotypic properties to explain the physiopathological behaviour of the different genotypes.

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Section: Discussionmentioning

confidence: 50%

Acidogenic and aciduric properties of Streptococcus mutans serotype c according to its genomic variability

Bedoya-Correa

Rincón‐Rodríguez

Parada-Sanchez

2021

European J Oral Sciences

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“…For a long time, the main bacteria strain considered cariogenic due to the production of acids and demineralization of the dental structure was S. mutans [ 74 , 94 , 102 ]. Instead, in recent decades, a more complex composition of the bacterial community associated with caries in its different stages of evolution has been considered and now includes the presence of Streptococcus sobrinus , S. salivarius , S. parasanguinis , Actinomyces and Lactobacillus spp.…”

Section: Resultsmentioning

confidence: 99%

Oral Microbiota, Its Equilibrium and Implications in the Pathophysiology of Human Diseases: A Systematic Review

et al. 2022

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Imbalances of the oral microbiota and dysbiosis have traditionally been linked to the occurrence of teeth and oral diseases. However, recent findings indicate that this microbiota exerts relevant influence in systemic health. Dysbiosis of the oral microbiota is implicated in the apparition and progression of cardiovascular, neurodegenerative and other major human diseases. In fact, the oral microbiota are the second most diverse and largely populated microbiota of the human body and its relationships with systemic health, although widely explored, they still lack of proper integration. The purpose of this systematic review is thus to widely examine the implications of oral microbiota in oral, cardiovascular and neurodegenerative diseases to offer integrative and up-to-date interpretations. To achieve that aim, we identified a total of 121 studies curated in PUBMED from the time interval January 2003–April 2022, which after careful screening resulted in 79 studies included. The reviewed scientific literature provides plausible vias of implication of dysbiotic oral microbiota in systemic human diseases, and encourages further research to continue elucidating the highly relevant and still poorly understood implications of this niche microbiota in systemic health. PROSPERO Registration Number: CRD42022299692. This systematic review follows relevant PRISMA guidelines.

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“…S. mutans is the main cariogenic bacterium in the early stages of caries. It forms biofilms that adhere to the tooth surface and cause tooth demineralization in the presence of fermentable carbohydrates ( Chen et al, 2020 ). It is necessary to inhibit the growth of S. mutans while occluding the dentinal tubules.…”

Section: Discussionmentioning

confidence: 99%

Effects of immunoglobulin Y-loaded amorphous calcium phosphate on dentinal tubules occlusion and antibacterial activity

Yan

Guan

Luo

et al. 2022

Front. Bioeng. Biotechnol.

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Aim: This study aimed to evaluate the effects of immunoglobulin Y (IgY)-loaded amorphous calcium phosphate (ACP) (IgY@ACP) on dentinal tubule occlusion and antibacterial activity.Methodology: IgY@ACP was synthesized based on a biomimetic mineralization strategy. The structure was examined by transmission electron microscopy and Fourier transform infrared spectroscopy. The IgY release property was assessed in vitro. The cell biocompatibility of IgY@ACP was evaluated by CCK-8. The dentin disks were prepared using healthy human molars, and their dentinal tubules were exposed to EDTA. Subsequently, they were randomly selected and treated with or without IgY@ACP for 7 days. The tubule occlusion morphologies and newly formed layers were observed by scanning electron microscopy (SEM) and x-ray diffraction, respectively. To evaluate the acid resistance and abrasion resistance of IgY@ACP, dentin disks that were treated for 1 day were immersed in acid solution or subjected to a toothbrush. The antibacterial effects against Streptococcus mutans (S. mutans) were evaluated by colony-forming unit (CFU) counting, adhesion property assessment, and crystal violet and live/dead bacterial staining. Finally, the occlusion effect was evaluated in rat incisors in vivo. One-way analysis of variance (ANOVA) was performed for statistical analysis. The level of significance was set at 0.05.Results: IgY@ACP presented an amorphous phase with a nanosize (60–80 nm) and sustained release of protein within 48 h. The CCK-8 results showed that IgY@ACP had good biocompatibility. After treatment with IgY@ACP for 1 day, the majority of dentinal tubules were occluded by a 0.3-μm-thick mineralized layer. Seven days later, all dentinal tubules were occluded by mineralization with a thickness of 1.4 μm and a depth of 16 μm. The newly mineralized layer showed hydroxyapatite-like diffraction peaks. In addition, IgY@ACP had good acid and abrasion resistance. After treatment with IgY@ACP, the CFU counting and adhesion rate of S. mutans were significantly reduced, the crystal violet staining was lighter, and the S. mutans staining revealed more dead cells. Most importantly, IgY@ACP had a certain occluding property in rat incisors in vivo.Conclusion: IgY@ACP can effectively occlude dentinal tubules with acid-resistant stability and has prominent anti-S. mutans effects, rendering it a potentially suitable desensitization material in the clinic.

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Isolation and Identification of Potentially Pathogenic Microorganisms Associated with Dental Caries in Human Teeth Biofilms

Cited by 19 publications

References 45 publications

Acidogenic and aciduric properties of Streptococcus mutans serotype c according to its genomic variability

Acidogenic and aciduric properties of Streptococcus mutans serotype c according to its genomic variability

Oral Microbiota, Its Equilibrium and Implications in the Pathophysiology of Human Diseases: A Systematic Review

Effects of immunoglobulin Y-loaded amorphous calcium phosphate on dentinal tubules occlusion and antibacterial activity

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