The Oxidative Metabolism of Eggs of Urechis Caupo

Rothschild, Lionel Walter Rothschild; Tyler, Albert

doi:10.2307/1539100

Cited by 11 publications

(6 citation statements)

References 23 publications

(30 reference statements)

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“…As previously reported (Rothschild and Tyler, 1958) and as noted here, the development of the eggs was not significantly accelerated or retarded in the CO-O 2 mixtures in the light. It might appear, then, that the energy released by the oxidation of the CO is not put to useful developmental work in this system.…”

Section: Discussionsupporting

confidence: 91%

“…The eggs from experiment No. 3 were not available for examination because of the acidification, but separate experiments on eggs run in the dark in CO-O 2 mixtures show only a small amount of inhibition of development, as reported previously (Rothschild and Tyler, 1958).…”

Section: Effect Of Co On Gas-uptake Of Eggs Of Ureehissupporting

confidence: 59%

“…The increase in gas-uptake reported by Rothschild and Tyler (1958) showed no significant difference in rate of development between those in 80% CO/CX and those in air. The eggs from experiment No.…”

Section: Effect Of Co On Gas-uptake Of Eggs Of Ureehismentioning

confidence: 71%

“…In some previous experiments (Rothschild and Tyler, 1958) with eggs of Urecliis, it was found that the rate of respiration in the presence of carbon monoxide (95% CO: 5% O L ,) in the light was greatly increased above that of the controls (95% N 2 :5% O 2 ). The average increase amounted to 85 per cent.…”

mentioning

confidence: 89%

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The Oxidation of Carbon Monoxide by Fertilized Eggs of Urechis Caupo Shown by Use of a C Label

Black

Epstein

Tyler

1958

The Biological Bulletin

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Section: Discussionsupporting

confidence: 91%

Section: Effect Of Co On Gas-uptake Of Eggs Of Ureehissupporting

confidence: 59%

Section: Effect Of Co On Gas-uptake Of Eggs Of Ureehismentioning

confidence: 71%

mentioning

confidence: 89%

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The Oxidation of Carbon Monoxide by Fertilized Eggs of Urechis Caupo Shown by Use of a C Label

Black

Epstein

Tyler

1958

The Biological Bulletin

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“…As Warburg (1927) pointed out, one would not expect a cell to show much inhibition of respiration in the presence of CO unless it was rapidly oxidizing substrate. Thus Runnstrom (1930Runnstrom ( , 1932Runnstrom ( ) and O'rstrom (1932 found that when the respiratory rate of Paracentrotus eggs was increased by means of dimethylparaphenylene diamine it could be inhibited by CO. Other evidence for the operation of a cytochrome system in eggs of Urechis has been previously presented (Rothschild and Tyler, 1958). A brief review is given there, and a more extended one by Runnstrom ( 1956) deals with this system in sea urchins.…”

Section: Respiratory Inhibition By Comentioning

confidence: 98%

EFFECTS OF FERTILIZATION AND DEVELOPMENT ON THE OXIDATION OF CARBON MONOXIDE BY EGGS OF STRONGYLOCENTROTUS AND URECHIS AS DETERMINED BY USE OF C¹³

Black

Tyler

1959

The Biological Bulletin

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In a previous publication. Black, Epstein and Tyler (1958) presented evide: obtained by the use of a C'^ label, for the oxidation of CO in fertilized eggs of the gephyrean worm. Urcchis caufo.In the light the oxidation of CO is superimpcon the ordinary respiration, so that manometrically an excess gas-uptake is observed.The eggs also oxidize CO in the dark, and this masks, in manometric experimer. :an inhibition of respiration by this compound.

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Electron transport in eggs, developing embryos and adult tissues of Spisula solidissima

Strittmatter

1961

J. Cell. Comp. Physiol.

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It has been shown, by both spectroscopic and enzymatic assays, that a number of tissues from certain marine molluscs contain cytochromes similar to the cytochrome components of the terminal electron transport pathway of mammals (Ball and Myerhof, '40; '57; Kawai, '59; Tappel, '60). Quantitative assays of the rate of oxygen consumption of whole eggs and developing embryos of the surf clam, Spisula solidissima, (Sclufer, '55) have also indicated that these marine eggs have an appreciable respiratory rate which is approximately doubled in 6-hour-old developing embryos. The purpose of the present study was to examine, in more detail, the terminal electron transport pathway in eggs, developing embryos and adult tissues of Spisula solidissima, and to determine whether or not qualitative or quantitative changes occur in this system of enzymes during embryonic differentiation. These experiments indicate that eggs, embryos and adult tissues of Spisula contain cellular particles resembling mitochondria in their terminal electron transport pathway. In addition, a second particulate fraction, which resembles liver microsomes in its oxidative enzyme pattern and absorption spectrum, has been isolated from unfertilized Spisula eggs. No qualitative or marked quantitative changes in the pattern of terminal electron transport has been observed during early embryonic differentiation, but adult heart muscle appeared to differ significantly from cggs and developing embryos in its succinic dehydrogenase activity and cytochrome complement. EXPERIMENTAL PROCEDUREThe experiments described below were carried out with Spisula solidissima collected during the summer months in the Woads Hole area. Eggs were harvested from Spisula ovaries and washed 6 or 7 times with approximately 200 ml of freshly filtered sea water. In each washing the eggs were allowed to settle through a 4 to 5 cm depth of sea water for 10 minutes and the sea water and immature eggs were then siphoned off. The washed egg volume was determined by centrifuging the eggs for 30 seconds at 1000 X g. For fertilization, the eggs were suspended in 100 volumes of sea water in wide finger bowls approximately 4 to 5 cm deep and 10 to 12 cm in diameter. From 0.4 to 0.8 ml of a 1 to 50 dilution of concentrated fresh sperm in sea water was added dropwise to each 200 ml of egg suspension with vigorous stirring to give a slight excess of active sperm over eggs. By this procedure 95 to 100% nuclear breakdown and from 75 to 95% synchronous division were usually achieved as described by Allen ('53 ).For growth of embryos, the fertilized eggs were permitted to develop at 23" in filtered sea water, with changes of sea water at 15 minutes after fertilization and, for older embryos, again at 10 hours after lpreliminary reports of this work have appeared (Strittmatter and Strittmatter, '60; Strittmatter et al., '60).

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The Oxidative Metabolism of Eggs of Urechis Caupo

Cited by 11 publications

References 23 publications

The Oxidation of Carbon Monoxide by Fertilized Eggs of Urechis Caupo Shown by Use of a C Label

The Oxidation of Carbon Monoxide by Fertilized Eggs of Urechis Caupo Shown by Use of a C Label

EFFECTS OF FERTILIZATION AND DEVELOPMENT ON THE OXIDATION OF CARBON MONOXIDE BY EGGS OF STRONGYLOCENTROTUS AND URECHIS AS DETERMINED BY USE OF C¹³

Electron transport in eggs, developing embryos and adult tissues of Spisula solidissima

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The Oxidative Metabolism of Eggs of Urechis Caupo

Cited by 11 publications

References 23 publications

The Oxidation of Carbon Monoxide by Fertilized Eggs of Urechis Caupo Shown by Use of a C Label

The Oxidation of Carbon Monoxide by Fertilized Eggs of Urechis Caupo Shown by Use of a C Label

EFFECTS OF FERTILIZATION AND DEVELOPMENT ON THE OXIDATION OF CARBON MONOXIDE BY EGGS OF STRONGYLOCENTROTUS AND URECHIS AS DETERMINED BY USE OF C13

Electron transport in eggs, developing embryos and adult tissues of Spisula solidissima

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EFFECTS OF FERTILIZATION AND DEVELOPMENT ON THE OXIDATION OF CARBON MONOXIDE BY EGGS OF STRONGYLOCENTROTUS AND URECHIS AS DETERMINED BY USE OF C¹³