The Oxidation of Carbon Monoxide by Fertilized Eggs of Urechis Caupo Shown by Use of a C Label

Black, Robert E.; Epstein, Samuel; Tyler, Albert

doi:10.2307/1539021

Cited by 7 publications

(6 citation statements)

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“…Such an agreement was found in the previously reported (Black et al, 1958) experiments on freshly fertilized eggs of Urechis. It is also found in the present data for Strongylocentrotus at all stages and the data for Urechis at the early stages.…”

Section: Data From Mass Spectrometersupporting

confidence: 92%

“…This was obtained from the Stable Isotopes Division of the Oak Ridge National Laboratories. The method employed was a modification of that described by Bernstein and Taylor (1947), as outlined in a previous paper (Black et al, 1958) . In this method the CO2 generated from the BaCO3 is passed over a zinc dust-asbestos fiber mixture heated to 520° C. in a combustion furnace.…”

Section: Eggsmentioning

confidence: 99%

“…

In a previous publication. Black, Epstein and Tyler (1958) presented evide: obtained by the use of a C'^ label, for the oxidation of CO in fertilized eggs of the gephyrean worm. Urcchis caufo.In the light the oxidation of CO is superimpcon the ordinary respiration, so that manometrically an excess gas-uptake is observed.The eggs also oxidize CO in the dark, and this masks, in manometric experimer.

…”

mentioning

confidence: 99%

“…In a previous publication. Black, Epstein and Tyler (1958) presented evide: obtained by the use of a C'^ label, for the oxidation of CO in fertilized eggs of the gephyrean worm. Urcchis caufo.…”

mentioning

confidence: 99%

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EFFECTS OF FERTILIZATION AND DEVELOPMENT ON THE OXIDATION OF CARBON MONOXIDE BY EGGS OF STRONGYLOCENTROTUS AND URECHIS AS DETERMINED BY USE OF C¹³

Black

Tyler

1959

The Biological Bulletin

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Section: Data From Mass Spectrometersupporting

confidence: 92%

Section: Eggsmentioning

confidence: 99%

“…

…”

mentioning

confidence: 99%

mentioning

confidence: 99%

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EFFECTS OF FERTILIZATION AND DEVELOPMENT ON THE OXIDATION OF CARBON MONOXIDE BY EGGS OF STRONGYLOCENTROTUS AND URECHIS AS DETERMINED BY USE OF C¹³

Black

Tyler

1959

The Biological Bulletin

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“…Usually the anaerobic glycolytic pathway typically replaces oxidative phosphorylation for ATP production and the maintenance of the intracellular ATP balance when mouse or human cells are cultured in media containing 10 μM sulfide, and the expression of nearly all of the genes in the anaerobic glycolytic pathway are up-regulated [ 39 , 40 ]. In U. unicinctus , it is confirmed that activities of key enzymes in the glycolytic pathway, phosphorylase, phosphofructokinase, and pyruvate kinase become markedly high after fertilization in U. unicinctus eggs [ 41 , 42 ], and enzymes associated with oxidative phosphorylation for ATP production have also been elucidated in echiuran respiration [ 43 – 47 ]. In this study, we identified a large number of genes related to glycolytic and oxidative phosphorylation pathways, such as pyruvate kinase, phosphorylase, phosphofructokinase, ADP-dependent glucokinase and phosphoglycerate kinase (Table 4 ).…”

Section: Discussionmentioning

confidence: 99%

Transcriptional response to sulfide in the Echiuran Worm Urechis unicinctus by digital gene expression analysis

Liu

Zhang

et al. 2015

BMC Genomics

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BackgroundUrechis unicinctus, an echiuran worm inhabiting the U-shaped burrows in the coastal mud flats, is an important commercial and ecological invertebrate in Northeast Asian countries, which has potential applications in the study of animal evolution, coastal sediment improvement and marine drug development. Furthermore, the worm can tolerate and utilize well-known toxicant-sulfide. However, knowledge is limited on the molecular mechanism of U. unicinctus responding to sulfide due to deficiency of its genetic information.MethodsIn this study, we performed Illumina sequencing to obtain the first Urechis unicinctus transcriptome data. Sequenced reads were assembled and then annotated using blast searches against Nr, Nt, Swiss-Prot, KEGG and COG. The clean tags from four digital gene expression (DGE) libraries were mapped to the U. unicinctus transcriptome. DGE analysis and functional annotation were then performed to reveal its response to sulfide. The expressions of 12 candidate genes were validated using quantitative real-time PCR. The results of qRT-PCR were regressed against the DGE analysis, with a correlation coefficient and p-value reported for each of them.ResultsHere we first present a draft of U. unicinctus transcriptome using the Illumina HiSeqTM 2000 platform and 52,093 unique sequences were assembled with the average length of 738 bp and N50 of 1131 bp. About 51.6 % of the transcriptome were functionally annotated based on the databases of Nr, Nt, Swiss-Prot, KEGG and COG. Then based on the transcriptome, the digital gene expression analysis was conducted to examine the transcriptional response to sulfide during 6, 24 and 48 h exposure, and finally 1705, 1181 and 1494 tag-mapped genes were identified as differentially expressed genes in the 6-h, 24-h and 48-h libraries, then were further subjected to pathway analyses.ConclusionsIn the DGE database of U. unicinctus, the alterations in certain known sulfide-related pathways indicate similar changes in response to sulfide. For more than 80 % of the identified pathway members, this is the first report on their association with sulfide stress, among which glycolysis pathway and PIDD involving pathways were unique and discussed in details, and were thought to play important roles in the sulfide tolerance of U. unicinctus. All the results are helpful to explain the mechanism of sulfide tolerance and detoxification.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-015-2094-z) contains supplementary material, which is available to authorized users.

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Co-insensitive respiration in mitochondria isolated from the eggs of the echiuroid, Urechis unicinctus

Tazawa¹

1986

Comparative Biochemistry and Physiology Part B: Comparative Bio

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The Oxidation of Carbon Monoxide by Fertilized Eggs of Urechis Caupo Shown by Use of a C Label

Cited by 7 publications

References 16 publications

EFFECTS OF FERTILIZATION AND DEVELOPMENT ON THE OXIDATION OF CARBON MONOXIDE BY EGGS OF STRONGYLOCENTROTUS AND URECHIS AS DETERMINED BY USE OF C¹³

EFFECTS OF FERTILIZATION AND DEVELOPMENT ON THE OXIDATION OF CARBON MONOXIDE BY EGGS OF STRONGYLOCENTROTUS AND URECHIS AS DETERMINED BY USE OF C¹³

Transcriptional response to sulfide in the Echiuran Worm Urechis unicinctus by digital gene expression analysis

Co-insensitive respiration in mitochondria isolated from the eggs of the echiuroid, Urechis unicinctus

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The Oxidation of Carbon Monoxide by Fertilized Eggs of Urechis Caupo Shown by Use of a C Label

Cited by 7 publications

References 16 publications

EFFECTS OF FERTILIZATION AND DEVELOPMENT ON THE OXIDATION OF CARBON MONOXIDE BY EGGS OF STRONGYLOCENTROTUS AND URECHIS AS DETERMINED BY USE OF C13

EFFECTS OF FERTILIZATION AND DEVELOPMENT ON THE OXIDATION OF CARBON MONOXIDE BY EGGS OF STRONGYLOCENTROTUS AND URECHIS AS DETERMINED BY USE OF C13

Transcriptional response to sulfide in the Echiuran Worm Urechis unicinctus by digital gene expression analysis

Co-insensitive respiration in mitochondria isolated from the eggs of the echiuroid, Urechis unicinctus

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EFFECTS OF FERTILIZATION AND DEVELOPMENT ON THE OXIDATION OF CARBON MONOXIDE BY EGGS OF STRONGYLOCENTROTUS AND URECHIS AS DETERMINED BY USE OF C¹³

EFFECTS OF FERTILIZATION AND DEVELOPMENT ON THE OXIDATION OF CARBON MONOXIDE BY EGGS OF STRONGYLOCENTROTUS AND URECHIS AS DETERMINED BY USE OF C¹³