The odontogenic differentiation of human dental pulp stem cells on hydroxyapatite-coated biodegradable nanofibrous scaffolds

Asghari, Fatemeh; Salehi, Roya; Agazadeh, Marziyeh; Adibkia, Khosro; Samiei, Mohammad; Akbarzadeh, Abolfazl; Aval, Negar Abbasi; Davaran, Soodabeh

doi:10.1080/00914037.2016.1163564

Cited by 44 publications

(41 citation statements)

References 32 publications

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“…The attachment and proliferation of hDPSCs on PCL/PLLA and PCL/PLLA/HA (HA; 0.1 and 0.5 wt %), scaffolds were fully investigated in previous work conducted by our team. 14 The hDPSCs are uniformly and abundantly distributed in the whole area of PCL/PLLA/SIM and PCL/PLLA/SIM/HA (HA; 0.1 and 0.5 wt %) scaffolds surface and exhibited normal cell adhesion (Figures 4a, 5a, 6e). Most of the cells appeared fibroblast-like morphology with multiple filopodia.…”

Section: Semmentioning

confidence: 99%

“…The effect of HA on PCL/PLLA nanofibrous scaffold was investigated in our team previous work. 14 The expression of BMP2 gene in the PLLA-PCL scaffold, after incorporation of simvastatin, was significantly higher than that in the control group and PLLA-PCL scaffold without simvastatin (P<0.01). In addition, the expression of BMP2 in the PLLA-PCL-HA (0.1)-SIM group was significantly higher than that in the control group and PLLA-PCL scaffold without simvastatin (P<0.001).…”

Section: Semmentioning

confidence: 99%

“…Alizarin red staining protocol and calcium mineral content quantization was described in our previous work 14 according to method was done previously by Stanford et al 17 In summary, the supernatant on the cells was removed and the scaffolds were rinsed twice with calcium and phosphate-free saline solution, and fixed with ice-cold 70% ethanol for 1 h. After a brief wash with water, the cells were tainted with 40 mM Alizarin red solution (pH 4.2) at ambient temperature for 15 min. The cells were washed with water for six times followed washing for 20 min with PBS (with rotation) to remove excess Alizarin red stain.…”

Section: Evaluation Of the Alizarin Red Activity After 14 Daysmentioning

confidence: 99%

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Osteogenic/Odontogenic Bioengineering with co-Administration of Simvastatin and Hydroxyapatite on Poly Caprolactone Based Nanofibrous Scaffold

Samiei¹,

Aghazadeh²,

Aminabadi³

et al. 2016

Adv Pharm Bull

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Section: Semmentioning

confidence: 99%

Section: Semmentioning

confidence: 99%

Section: Evaluation Of the Alizarin Red Activity After 14 Daysmentioning

confidence: 99%

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Osteogenic/Odontogenic Bioengineering with co-Administration of Simvastatin and Hydroxyapatite on Poly Caprolactone Based Nanofibrous Scaffold

Samiei¹,

Aghazadeh²,

Aminabadi³

et al. 2016

Adv Pharm Bull

Self Cite

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“…These biodegradable scaffolds provide a suitable three-dimensional structure required for cell adhesion and cell delivery to specific sites [15]. Meanwhile, multipotent stem cells could differentiate into specific cell lineages [16]. Human dental pulp stem cells with a potential of odontogenic and osteogenic differentiation are one of the sufficient and available sources for tissue engineering [17,18].…”

Section: Introductionmentioning

confidence: 99%

Towards osteogenic differentiation of human dental pulp stem cells on PCL-PEG-PCL/zeolite nanofibrous scaffolds

Alipour

Aghazadeh

Akbarzadeh

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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Presently, tissue engineering has been developed as an effective option in the restoration and repair of tissue defects. One of the tissue engineering strategies is to use both biodegradable scaffolds and stimulating factors for enhancing cell responses. In this study, the effect of zeolite was assessed on cell viability, proliferation, osteo/odontogenic differentiation, and mineralization of human dental pulp stem cells (hDPSCs) cultured on poly (e-coprolactone)poly (ethylene glycol)-poly (e-caprolactone) (PCL-PEG-PCL) nanofibers. For this purpose, PCL-PEG-PCL nanofibrous scaffolds incorporated with zeolite were prepared via electrospinning. Both PCL-PEG-PCL and PCL-PEG-PCL/Zeolite nanofibrous scaffolds revealed bead-less constructions with average diameters of 430 nm and 437 nm, respectively. HDPSCs were transferred to PCL-PEG-PCL nanofibrous scaffolds containing zeolite nanoparticles. Cell adhesion and proliferation of hDPSCs and their osteo/odontogenic differentiation on these scaffolds were evaluated using MTT assay, Alizarin red S staining, and qRT-PCR assay. The results revealed that PCL-PEG-PCL/Zeolite nanofibrous scaffolds could support better cell adhesion, proliferation and osteogenic differentiation of hDPSCs and as such is expected to be a promising scaffold for bone tissue engineering applications.

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“…Tissue regeneration needs an appropriate microenvironment for the adhesion, proliferation differentiation and recruitment of cells, and this can be facilitated by the use of biomimetic materials [15]. Tissue engineering for cartilage needs a simulated ECM that stimulates the cells to proliferate and differentiate towards new tissue generation [2,16].…”

Section: Introductionmentioning

confidence: 99%

Nanocomposite hydrogels for cartilage tissue engineering: a review

Asadi

Alizadeh

Salehi

et al. 2017

Artificial Cells, Nanomedicine, and Biotechnology

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100

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The loss of cartilaginous tissues is an important challenge to orthopaedic surgeons. Injury to cartilage tissue due to its properties is along with movement difficulties. Tissue engineering is a developing field that can be used for regeneration or replacement of damaged tissues. In this field, an appropriate scaffold that support the recruitment, adhesion, proliferation and differentiation of cells is necessary. Hydrogels recently considered as materials that resemble the extracellular matrix (ECM) and efficiently replace defective tissues, but they have limited mechanical strength. So nanomaterials are embedded in the hydrogel's matrix to improve their properties. Nanoparticles, such as organic/polymeric and inorganic (hydroxyapatite, clay, graphene and metallic nanoparticles), can be used as fillers to reinforce the hydrogel matrix. Utilizing those nanocomposites could help in better performance of hydrogels applicable in cartilage regeneration practices. This review presents some of nanocomposite hydrogel (NCH) systems that used in cartilage tissue engineering.

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The odontogenic differentiation of human dental pulp stem cells on hydroxyapatite-coated biodegradable nanofibrous scaffolds

Cited by 44 publications

References 32 publications

Osteogenic/Odontogenic Bioengineering with co-Administration of Simvastatin and Hydroxyapatite on Poly Caprolactone Based Nanofibrous Scaffold

Osteogenic/Odontogenic Bioengineering with co-Administration of Simvastatin and Hydroxyapatite on Poly Caprolactone Based Nanofibrous Scaffold

Towards osteogenic differentiation of human dental pulp stem cells on PCL-PEG-PCL/zeolite nanofibrous scaffolds

Nanocomposite hydrogels for cartilage tissue engineering: a review

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