The Occurrence of Blood‐Group Specific Material in the Plasma and Serum of Stored Blood

Abstract

Summary The plasma and serum of stored group A blood were studied for the presence of blood‐group active material. It was found that, over the periods examined, there was an appreciable rise in the A inhibiting activity of both plasma and serum. It is concluded that blood‐group active material from the red cell appears in the serum or plasma with the aging of blood in vitro. Résumé Le plasma et le sérum du sang conservé ont été étudiés pour y déterminer la presence de substances de groupe actives. Il a été trouvé que, pendant la période d'examen, il y a une augmentation appréciable de l'activité inhibitrice A dans le plasma et le sérum. On peut donc conclure que les substances de groupe actives apparaissent dans le sérum ou le plasma à partir des érythrocytes à mesure que le sang vieillit in vitro Zusammenfassung Das Plasma sowie das Serum von gelagertem A‐Blut wurde auf den Gehalt an gelüster A‐Substanz untersucht. Dabei zeigte sich, daß mit zunehmender Dauer der Lagerung der Gehalt an A‐Substanz erheblich zunimmt. Offenbar geht bei Lagerung in vitro mit der Zeit ein Teil der Erythrozytenantigene in Lüsung über.

The how and why of exocytic vesicles

“…66 Weed and coworkers [67][68][69][70][71][72] proposed that fragmentation of the RBC membrane was the mechanism both in vivo and in vitro. In 1964, Michel 36 reported that blood group-specific material was present in the plasma of stored blood. In 1969, Cooper and Jandl 73 reported that RBCs lost cholesterol and phospholipids when incubated in serum without glucose, not suspecting that vesicle formation was part of the phenomenon.…”

“…The microvesicles shed during storage contain lipids, band 3 protein, glycophorins, and actin, but lack spectrin and ankyrin 33 . Microvesicles shed during storage have blood group antigens A, B, H, and P 1 34‐36 and D, c, e, and Fy a 37 . The activities of M and N are slight, which may be due to insufficient antigen sites on the microvesicle 34,37 …”

Blood Group Substances A and B

“…AUBERT et al [2], HOSTRUP [8,9] and JAKOBOWICZ and BRYCE [lo] showed by hemagglutination inhibition procedures that human serum contains blood group substances. MICHEL [12] found that filtered plasma from group A blood collected in ACD solution and stored at 4°C showed il gradual increase in blood group substance upon storage and reached a level 64 times that of the fresh plasma after 12 weeks. HOMBURGER and MELIN [7] demonstrated that human plasma and many of its fractions inhibited the activity of isoagglutinins.…”

Changes in the Agglutinability of Red Cells and the Inhibition of Specific Agglutination by Plasma from Human Blood Taken into ACD and Stored at 4 4DgC

“…for compatibility testing and antibody identification procedures. Changes in the amount of blood group A substance in plasma from stored blood has been described [7]. However, very little work on the presence of other blood group substances appears to have been carried out.…”