The occurrence and rapid discrimination of Fomes fomentarius genotypes by ITS-RFLP analysis

Judova, Jana; Dubikova, Katarina; Gáperová, Svetlana; Gáper, Ján; Pristaš, Peter

doi:10.1016/j.funbio.2011.10.010

Cited by 22 publications

(23 citation statements)

References 21 publications

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“…In our previous work (Judova et al 2012) we observed genetic variability in F. fomentarius based on comparison of ITS sequences indicating that F. fomentarius population in Central Europe is probably composed of two sympatric cryptic species differing by 3% at the ITS sequence level. However, a relatively high level of intragenomic variation of ITS sequences was reported for some species, e.g.…”

Section: Discussionmentioning

confidence: 88%

“…All DNA techniques used were in principle as already described (Judova et al 2012). Shortly, total genomic DNA of F. fomentarius strains was prepared using microwave treatment according to Goodwin & Lee (1993) with small modifications.…”

Section: Dna Isolation and Analysismentioning

confidence: 99%

“…In our previous work (Judova et al 2012) we observed the existence of two genotypes in this species based on comparison of internal transcribed spacer (ITS) sequences. Two genotypes differ from each other by 3% at the nucleotide level and the presence of 7 bp indel in the ITS2 region is the most remarkable difference.…”

Section: Introductionmentioning

confidence: 99%

“…Two genotypes differ from each other by 3% at the nucleotide level and the presence of 7 bp indel in the ITS2 region is the most remarkable difference. A rapid method was developed for discrimination of these genotypes and analyses suggest that one of genotypes is prevalent on beech (F. sylvatica), while other prefers other broadleaves trees (Judova et al 2012).…”

Section: Introductionmentioning

confidence: 99%

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Genetic variability in Fomes fomentarius reconfirmed by translation elongation factor 1-α DNA sequences and 25S LSU rRNA sequences

et al. 2013

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Abstract:The existence of two cryptic species within strains of the wood-decaying fungus Fomes fomentarius was revealed recently based on the internal transcribed spacer (ITS) sequence variability. In this study for the first time the sequences of another molecular markers, partial translation elongation factor 1-α (efa) region and partial 25S large subunit ribosomal RNA gene were obtained and used to evaluate genetic variability of F. fomentarius. Congruent phylogeny was observed for all three markers used confirming the presence of two cryptic species within F. fomentarius. Surprisingly, ITS sequence variability within F. fomentarius was significantly lower compared to the variability of efa sequences (0.023 versus 0.036 nucleotide substitutions per site) questioning the discriminatory power of ITS sequences for fungal species identification.

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Section: Discussionmentioning

confidence: 88%

Section: Dna Isolation and Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Genetic variability in Fomes fomentarius reconfirmed by translation elongation factor 1-α DNA sequences and 25S LSU rRNA sequences

et al. 2013

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“…, Judova et al. ). Fomes fomentarius was the only species detected from willow trees showing wood decay symptoms.…”

Section: Resultsmentioning

confidence: 97%

Identification and characterization of fungi associated with internal wood lesions and decline disease of willow and poplar trees in Iran

Hashemi

Mohammadi

2016

Forest Pathology

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Summary During the study of fungal trunk pathogens associated with urban trees decline in Shiraz (Iran), a serious decline of willow and poplar trees was observed. Therefore, an investigation was conducted on these trees in some areas of this city during spring and summer 2012 and 2013, to determine the main fungal trunk pathogens associated with these ornamental plants. Plant materials were collected from trees exhibiting disease symptoms such as yellowing, shoot canker, shoot dieback, defoliation and internal wood necrosis and decayed wood. Fungal isolations were made from discoloured or decayed wood tissue onto 2% malt extract agar (MEA) amended with streptomycin sulphate. Nine species, Fomes fomentarius, Diplodia seriata, Lasiodiplodia theobromae, Dothiorella sarmentorum, Neoscytalidium hyalinum, Diatrype whitmanensis, Phaeoacremonium rubrigenum, P. aleophilum and P. parasiticum, were identified based on morphology and DNA sequence comparisons. Pathogenicity tests were performed on detached shoots of willow and poplar trees under greenhouse conditions. Lasiodiplodia theobromae caused the longest lesions on willow. On poplar shoots, the longest lesions were caused by P. parasiticum. Diplodia seriata produced the smallest lesions on both woody hosts. First reports from willow wood include P. parasiticum, P. rubrigenum, D. whitmanensis, L. theobromae, D. seriata and N. hyalinum, while new reports from poplar wood include P. parasiticum and Do. sarmentorum. Based on our knowledge, this is also the first report of D. whitmanensis in Iran.

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Emergent Tools and Techniques in Diagnosis of Soil-Borne Phytopathogens

Singh¹,

Bhoi²,

Majhi³

et al. 2023

Detection, Diagnosis and Management of Soil-Borne Phytopathogens

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The occurrence and rapid discrimination of Fomes fomentarius genotypes by ITS-RFLP analysis

Cited by 22 publications

References 21 publications

Genetic variability in Fomes fomentarius reconfirmed by translation elongation factor 1-α DNA sequences and 25S LSU rRNA sequences

Genetic variability in Fomes fomentarius reconfirmed by translation elongation factor 1-α DNA sequences and 25S LSU rRNA sequences

Identification and characterization of fungi associated with internal wood lesions and decline disease of willow and poplar trees in Iran

Emergent Tools and Techniques in Diagnosis of Soil-Borne Phytopathogens

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