Genetic variability in Fomes fomentarius reconfirmed by translation elongation factor 1-α DNA sequences and 25S LSU rRNA sequences

Pristaš, Peter; Gáperová, Svetlana; Gáper, Ján; Judova, Jana

doi:10.2478/s11756-013-0228-9

Cited by 11 publications

(11 citation statements)

References 17 publications

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“…However, our and other previous phylogenetic analyses indicate that Fomes diversity is higher than currently assumed (McCormick et al 2013;Pristas et al 2013). Phylogenetic analyses indicate at least one new Fomes species from Asia, and a potential new species from North America (F. fomentarius II in McCormick et al 2013).…”

Section: Potential Diversity In the Genus Fomescontrasting

confidence: 53%

“…Grienke et al (2014) extensively reviewed the applications of F. fomentarius in traditional medicine and the current knowledge on its metabolite profile. Recent phylogenetic analyses based on multiple genetic markers indicated that F. fomentarius possibly contained cryptic species (Pristas et al 2013). Our earlier study also indicated that a European lineage could possibly represent a separate species that could be differentiated based on growth characteristics and substrate differences (Dresch et al 2015).…”

Section: Introductionmentioning

confidence: 65%

“…This has led to the description of cryptic species representing distinct phylogenetic lineages (Krüger et al 2004;Geml et al 2006;Balasundaram et al 2015;Obase et al 2016;Sanchez-Garcia et al 2016;Dowie et al 2017;Mukhin et al 2018). Meanwhile, multi-gene phylogenies have proven to be especially reliable for species definition, confirming several of these cryptic taxa, as in Amanita and Fomes (Pristas et al 2013;Balasundaram et al 2015). In this context it is especially important to screen for distinguishing characters, and to test them in a statistically significant number.…”

Section: Cryptic Species Revisitedmentioning

confidence: 99%

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How to resolve cryptic species of polypores: an example in Fomes

et al. 2019

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Species that cannot be easily distinguished based on morphology, but which form distinct phylogenetic lineages based on molecular markers, are often referred to as cryptic species. They have been proposed in a number of fungal genera, including the basidiomycete genus Fomes. The main aim of this work was to test new methods for species delimitation in cryptic lineages of polypores, and to define useful characters for species identification. A detailed examination of a number of different Fomes strains that had been collected and isolated from different habitats in Italy and Austria confirmed the presence of distinct lineages in the Fomes fomentarius clade. Our zero hypothesis was that the Mediterranean strains growing on Quercus represent a species which can be delimited based on morphological and physiological characters when they are evaluated in statistically relevant numbers. This hypothesis was tested based on phylogenetic analysis of the rDNA ITS region, morphological characters of basidiomes and pure cultures, growth rates and optimum growth temperature experiments, mycelial confrontation tests, enzyme activity tests and volatile organic compound (VOC) production. The Mediterranean lineage can unambiguously be delimited from F. fomentarius. A syntype of an obscure and previously synonymized name, Polyporus inzengae, represents the Mediterranean lineage that we recognize as Fomes inzengae, a distinct species. The rDNA ITS region is useful for delimitation of Fomes species. Moreover, also a variety of morphological characters including hymenophore pore size, basidiospore size, and diameter of skeletal hyphae are useful delimiting characters. The ecology is also very important, because the plant host appears to be a central factor driving speciation. Physiological characters turned also out to be species-specific, e.g. daily mycelial growth rates or the temperature range of pure cultures. The production of VOCs can be considered as a very promising tool for fast and reliable species delimitation in the future.

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Section: Potential Diversity In the Genus Fomescontrasting

confidence: 53%

Section: Introductionmentioning

confidence: 65%

Section: Cryptic Species Revisitedmentioning

confidence: 99%

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How to resolve cryptic species of polypores: an example in Fomes

et al. 2019

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“…Based on the ITS region, Judova et al () have suggested that populations of F. fomentarius are comprised of two sympatric cryptic species; this has been confirmed by Pristas, Gaperova, Gaper, and Judova () using the efa gene. One genotype, termed genotype A, has been suggested to be prevalent on European beech while the other, termed genotype B, is additionally found on other host species (Judova et al, ).…”

Section: Discussionmentioning

confidence: 83%

Arthropod communities in fungal fruitbodies are weakly structured by climate and biogeography across European beech forests

Frieß

Müller

Aramendi³

et al. 2019

Diversity and Distributions

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Aim:The tinder fungus Fomes fomentarius is a pivotal wood decomposer in European beech Fagus sylvatica forests. The fungus, however, has regionally declined due to centuries of logging. To unravel biogeographical drivers of arthropod communities associated with this fungus, we investigated how space, climate and habitat amount structure alpha and beta diversity of arthropod communities in fruitbodies of F. fomentarius.Location: Temperate zone of Europe. Taxon: Arthropods.Methods: We reared arthropods from fruitbodies sampled from 61 sites throughout the range of European beech and identified 13 orders taxonomically or by metabarcoding. We estimated the total number of species occurring in fruitbodies of F. fomentarius in European beech forests using the Chao2 estimator and determined the relative importance of space, climate and habitat amount by hierarchical partitioning for alpha diversity and generalized dissimilarity models for beta diversity. A subset of fungi samples was sequenced for identification of the fungus' genetic structure. Results:The total number of arthropod species occurring in fruitbodies of F. fomentarius across European beech forests was estimated to be 600. Alpha diversity increased with increasing fruitbody biomass; it decreased with increasing longitude, temperature and latitude. Beta diversity was mainly composed by turnover. Patterns of beta diversity were only weakly linked to space and the overall explanatory power was low. We could distinguish two genotypes of F. fomentarius, which showed no spatial structuring. Main conclusion:Fomes fomentarius hosts a large number of arthropods in European beech forests. The low biogeographical and climatic structure of the communities suggests that fruitbodies represent a habitat that offers similar conditions across large gradients of climate and space, but are characterized by high local variability in community composition and colonized by species with high dispersal ability. For European beech forests, retention of trees with F. fomentarius and promoting its recolonization where it had declined seems a promising conservation strategy. K E Y W O R D Sdead wood,

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“…The reaction mixtures (50 µL) contained 5 µL of 10× concentrated complete Taq Reaction Buffer, 200 µM of each dNTP, 1.25 U Taq DNA polymerase (Taq Core Kit; Jena Bioscience), 1 µM of each primer, and 50 ng of DNA template. For amplification of TEF1-α sequences, the primers and conditions specified in [27] were used. The β-tubulin gene was partially amplified with primer pair T1 and T22 using cycling conditions described previously [28].…”

Section: Dna Extractionmentioning

confidence: 99%

New Endophytic Fusarium spp. from Fraxinus excelsior Leaves in Slovakia

et al. 2020

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Fraxinus excelsior is currently suffering from ash dieback disease caused by the fungus Hymenoscyphus fraxineus. Co-occurrence of large numbers of other fungi with endophytic as well as pathogenic lifestyles has been observed on F. excelsior. In this study, new endophytic Fusarium spp. isolate Fraxinus excelsior Fusarium from Slovak territory, associated with F. excelsior, was characterised. Using morphology-based approaches, the fungus was assigned to the genus Fusarium. The isolate formed thick-walled hyaline, fusiform, slightly curved macroconidia, produced from monophialides in sporodochia, pointed at the tip, mostly 3 – 5 septate, occasionally 6 septate, and 40 – 68 × 3.8 – 5.0 µm in size with basal pedicellate cells. For more accurate taxonomic affiliation, molecular sequence analyses of the internal transcribed spacer, translation elongation factor 1-alpha sequences, and partial β-tubulin gene were performed. While the internal transcribed spacer sequence of the isolate showed the highest similarity to the Fraxinus oxysporum species, translation elongation factor 1-alpha and partial β-tubulin sequences were distantly related to the Fraxinus avenaceum species. Based on these data, the Fraxinus excelsior Fusarium isolate could probably be considered a new species of the Fusarium genus.

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Genetic variability in Fomes fomentarius reconfirmed by translation elongation factor 1-α DNA sequences and 25S LSU rRNA sequences

Cited by 11 publications

References 17 publications

How to resolve cryptic species of polypores: an example in Fomes

How to resolve cryptic species of polypores: an example in Fomes

Arthropod communities in fungal fruitbodies are weakly structured by climate and biogeography across European beech forests

New Endophytic Fusarium spp. from Fraxinus excelsior Leaves in Slovakia

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