The nucleotide sequence of bacteriophage T5 DNA at the region between early and late genes

Av, Kaliman; Vm, Kryukov; Aa, Bayev

doi:10.1093/nar/16.13.6230

Cited by 5 publications

(2 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Downstream from the stop codon TAA there is a strong bidirectional rho-independent terminator of transcription (at nucleotides 4,6914,721 in Fig. 2) [7,8]. This terminator is situated at the boundary between the early and late phage T5 genes [6], An analysis of the LTF protein sequence revealed high content of Ser (10.7%), Gly (10.2%), Ala (10.2%) and Thr (7.1%).…”

Section: S G a S A P A I R A M W C D G S L A D T T R Y L G A T Q P 11mentioning

confidence: 98%

“…It is situated at the left end of the late T5 gene region near the boundary of the early and late genes, and their transcription is accomplished from different DNA strands in the opposite directions [6]. The strong transcription terminator from the boundary region (67.2% of the T5 DNA length) has been sequenced and characterized in our previous papers [7,8].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The nucleotide sequence of the bacteriophage T5 ltf gene

et al. 1995

View full text Add to dashboard Cite

Section: S G a S A P A I R A M W C D G S L A D T T R Y L G A T Q P 11mentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

The nucleotide sequence of the bacteriophage T5 ltf gene

et al. 1995

View full text Add to dashboard Cite

The nucleotide sequence of the region of bacteriophage T5 early gene D10–D15

1988

View full text Add to dashboard Cite

Lytic conversion of Escherichia coli by bacteriophage T5: blocking of the FhuA receptor protein by a lipoprotein expressed early during infection

Decker¹,

Krauel²,

Meesmann³

et al. 1994

Molecular Microbiology

View full text Add to dashboard Cite

The nucleotide sequence of the region between the oad gene, encoding the host specificity protein, and the right-terminal repetition of bacteriophage T5 DNA was determined. Five small open reading frames, the first of which was called llp, were detected, which apparently formed an operon transcribed from a promoter that overlapped the oad promoter. Both promoters were confirmed by primer extension assays. Using mRNA isolated at different times after T5 infection, the llp and oad promoters were identified as early and late promoters, respectively. The N-terminus of the llp gene product possess a signal sequence and a processing site characteristic of lipoproteins. After subcloning and expression of llp, its product Llp was identified as a 7.8 kDa polypeptide. Acylation of Llp was confirmed by addition of globomycin, which resulted in the accumulation of the unprocessed precursor form. FhuA+ cells synthesizing Llp were resistant to phage T5. Resistance was caused by inhibition of adsorption of T5 to its FhuA receptor protein. Resistance could be overcome by derepression of fhuA transcription, suggesting a blocking of FhuA by direct interaction with Llp. Since Llp-mediated T5 resistance has several aspects in common with the phenomenon of lysogenic conversion, we suggest that it should be called lytic conversion.

show abstract

The nucleotide sequence of bacteriophage T5 DNA at the region between early and late genes

Abstract: The nucleotide sequence of bacteriophage T5 DNA at the region between early and late genes (66.1 -68.6 X of the T5 DNA length (1,21)

Cited by 5 publications

References 0 publications

The nucleotide sequence of the bacteriophage T5 ltf gene

The nucleotide sequence of the bacteriophage T5 ltf gene

The nucleotide sequence of the region of bacteriophage T5 early gene D10–D15

Lytic conversion of Escherichia coli by bacteriophage T5: blocking of the FhuA receptor protein by a lipoprotein expressed early during infection

Contact Info

Product

Resources

About